All solvents were of high-performance LC grade. Acetonitrile, formic acid and dopamine were obtained from Sigma Aldrich (St. Louis, MO, USA). Working solutions were prepared in ultrapure water provided by a Milli-Q system (Millipore, Bedford, MA, USA). Each analytical stock solution (1 mg/ mL) was prepared in 0.111 M ascorbic acid in a 1:1 mixture solution to prevent oxidation and stored at 80 °C. A working internal standard solution (buffer A), 20 pg/μL of isoproterenol (Nacalai, Japan) was prepared in buffer B (50% Acetonitrile,0.1% formic acid, and 0.111 M ascorbic acid) immediately before analysis. Stock of 10 mg/mL dopamine standard were diluted in buffer A, followed by calibration curve in which dopamine stock was diluted in buffer A according to corresponding concentration (0.025, 0.25, 1.25, 1.5, 2.0, 2.5, 4.0 pg/μL).
Isoproterenol
Manufactured by Nacalai Tesque
Sourced in Japan
Isoproterenol is a synthetic catecholamine that acts as a non-selective beta-adrenergic agonist. It is commonly used in laboratory research as a reference standard and pharmacological tool to study the effects of stimulating beta-adrenergic receptors.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Merck Group (3 mentions)
Acetonitrile, by Merck Group (3 mentions)
Dopamine, by Merck Group (1 mentions)
Acetonitrile, by Nacalai Tesque (1 mentions)
Formic acid, by Nacalai Tesque (1 mentions)
Milli q system, by Merck Group (1 mentions)
Cyanidin chloride, by Fujifilm (1 mentions)
Forskolin, by Nacalai Tesque (1 mentions)
Pdgf bb, by Fujifilm (1 mentions)
Isoproterenol, by Merck Group (1 mentions)
Cosmospin filter g, by Nacalai Tesque (1 mentions)
Sb c18 column, by Agilent Technologies (1 mentions)
5 hiaa, by Merck Group (1 mentions)
Agilent masshunter quantitative analysis software, by Agilent Technologies (1 mentions)
Serotonin, by Merck Group (1 mentions)
Carbenoxolone, by Merck Group (1 mentions)
Diltiazem, by Fujifilm (1 mentions)
Lidocaine, by Merck Group (1 mentions)
5 protocols using isoproterenol
1
Dopamine Quantification in Ascorbic Acid
2
Compound Sourcing for Cellular Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Test compounds used in this study were
purchased from the following manufacturers: cyanidin 3-glucoside (Fuji
Film Wako Pure Chemical Corporation, Japan, #633-42451NS380101), cyanidin
chloride (Fuji Film Wako Pure Chemical Corporation, Japan, #030-21961),
platelet-derived growth factor-BB (PDGF-BB) (Fuji Film Wako Pure Chemical
Corporation, Japan, #166-19743), forskolin (Nacalai Tesque, Inc.,
Japan, #16384-84), isoproterenol (Nacalai Tesque, Inc., Japan, #19703-04),
and H89 (Cayman Chemical, United States, #10010556).
purchased from the following manufacturers: cyanidin 3-glucoside (Fuji
Film Wako Pure Chemical Corporation, Japan, #633-42451NS380101), cyanidin
chloride (Fuji Film Wako Pure Chemical Corporation, Japan, #030-21961),
platelet-derived growth factor-BB (PDGF-BB) (Fuji Film Wako Pure Chemical
Corporation, Japan, #166-19743), forskolin (Nacalai Tesque, Inc.,
Japan, #16384-84), isoproterenol (Nacalai Tesque, Inc., Japan, #19703-04),
and H89 (Cayman Chemical, United States, #10010556).
3
Quantifying 5-HT and 5-HIAA in Tilapia Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
5-HT and 5-HIAA measurement in male tilapia brain area 2 and 3 (control = 8, defeat = 8) was carried out using liquid chromatography-mass spectrometry (the Agilent Technologies 6410 Triple Quad Liquid chromatography-mass spectrometry equipped with a ZORBAX SB- C18 column). The procedures were as described previously (4 (link)). Briefly, the brain tissues were homogenized with 350 μL of 20 ng/mL isoproterenol (internal standard, Nacalai Tesque) in 50% acetonitrile (Fisher Scientific) with 0.1% formic acid (Sigma). The supernatant was filtered by Cosmospin filter G (Nacalai Tesque). A standard solution of 5-HT, 5-HIAA (Sigma), 5-HIAA, and 20 ng/mL isoproterenol was prepared in 50% acetonitrile with 0.1% formic acid in the range of 1.25 to 160 ng/mL. The production of 5-HT, 5-HIAA, and isoproterenol was observed for further analysis (m/z 177.1–160 for 5-HT, m/z 192.07–146 for 5-HIAA, and m/z 212.1–194 for isoproterenol). Data acquisition software Agilent Masshunter Quantitative Analysis software (RRID: SCR_015040, Agilent Technologies) was used for data analysis.
4
Serotonin Quantification in Analytical Solution
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All solvents were of high-performance LC grade. Acetonitrile, formic acid and serotonin were obtained from Sigma Aldrich (St. Louis, MO, USA). Reagents for the analytical solution were prepared using 50% Acetonitrile with 0.1% formic acid, which was supplemented with 0.111 M ascorbic acid to prevent oxidation of analytes. The 5-HT standard solutions (1.25, 2.5, 5, 10, 20, 40, 80, 160 ng/ml) were prepared in the analytical solution containing 20 pg/µl of isoproterenol (Nacalai Tesque, Japan) as an internal standard.
5
Compound Screening in hiPS-CMs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lidocaine and carbenoxolone were purchased from Sigma-Aldrich (St. Louis, MO, USA). Diltiazem was purchased from Wako Pure Chemical Industries (Osaka, Japan). Isoproterenol was purchased from Nacalai Tesque Inc. (Kyoto, Japan) or Sigma-Aldrich (St. Louis, MO, USA). The test articles were dissolved in the DMSO to prepare stock solutions that were 1000-fold the final concentrations to be applied. As for the hiPS-CMs experiments, the stock solutions at a volume of 2 μL were applied to each well containing the medium at 2 mL. In the guinea-pig Langendorff experiments, the stock solutions were diluted 1000-fold with the KH solutions immediately before use.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!