R 210 rotavapor system

Manufactured by Büchi

The R-210 Rotavapor System is a rotary evaporator designed for the efficient and controlled removal of solvents from samples. It features a heated water bath, a vapor duct, and a condenser to facilitate the evaporation process. The system is suitable for a wide range of applications that require the concentration or isolation of substances from liquid solutions.

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Lab products found in correlation

Whatman filter paper, by Cytiva (1 mentions) No 1 lter paper, by Cytiva (1 mentions)

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Rotavapor (188 citations) R-210 (151 citations) Rotavapor R-210 (143 citations) Rotavapor system (2 citations)

4 protocols using r 210 rotavapor system

Sterile Peppermint Extract Preparation

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10 g of powdered peppermint were macerated in 100 mL of a hydroethanolic solvent (80/20, solvent/water, v/v) for 6 h. Then, the macerate was filtered via Wattman N°1 paper and evaporated using rotavapor (Buchi R‐210 Rotavapor System) at 45°C (Sultana et al., 2009 (link)).
The extract obtained was placed in sterilized, sealed glass vials, sterilized by autoclaving at 120°C for 15 min, and then cooled (Ammendola et al., 2020 (link)).
The sterility of the extract was confirmed by adding 2 mL of extract to 10 mL of Mueller Hinton. The absence of microbial growth in the broth after incubation at 37°C for 24 h indicates that the extract is sterile.

Guemidi C., Ait Saada D., Ait Chabane O., Elmastas M., Erenler R., Yilmaz M.A., Tarhan A., Akkal S, & Khelifi H. (2024). Enhancement of yogurt functionality by adding Mentha piprita phenolic extract and evaluation of its quality during cold storage. Food Science & Nutrition, 12(4), 3007-3020.

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Microwave-Assisted Extraction of Boesenbergia rotunda

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Six grams of powdered B. rotunda (Materia Medika, Batu, East Java, Indonesia) and distilled water or 96% ethanol in a ratio of 1 : 10 were put in a vessel of MAE (microwave-assisted extraction) (Anton-Paar). MAE was operated according to the specified protocol (holding temperature, 50°C; 5 min warming up, 50°C; time holding, 10 min; 5 min cooling down; power, 1500 W). The extract was filtered using the Whatman filter paper and then evaporated using a Buchi R-210 Rotavapor System (50 rpm, 37°C). The obtained extract was stored at 4°C.

Widyananda M.H., Wicaksono S.T., Rahmawati K., Puspitarini S., Ulfa S.M., Jatmiko Y.D., Masruri M, & Widodo N. (2022). A Potential Anticancer Mechanism of Finger Root (Boesenbergia rotunda) Extracts against a Breast Cancer Cell Line. Scientifica, 2022, 9130252.

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Tomato Extract Preparation: Solvent Extraction

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Ripe red tomatoes (approximately 850 g) were cut into small pieces and then homogenized in a mixer grinder for 30 s to obtain a homogenous pulp. Solvent extraction (Liquid-liquid extraction) was carried out to prepare the tomato extract. About 1200 mL of the solvents, such as hexane, ethanol, and acetone in the ratio of 2:1:1 were added to the 2000 mL flask containing 900 mL of homogenized tomato pulp. The flask was stoppered, and agitation was provided for 10 min. Then 300 mL of double distilled water was added to the mixture and again agitation was provided for 5 min. The mixture was left undisturbed at room temperature for 15 min to allow phase separation. The mixture separated into two layers: distinct polar layer (900 mL) and nonpolar layer (600 mL). The upper nonpolar layer was separated using separatory funnel. Then it was concentrated using Buchi R-210 rotavapor system and dried under vacuum. Finally, 124 mg of TLE was obtained.

Thulasidas J.S., Varadarajan G.S., Camarillo I., Mittal L, & Sundararajan R. (2023). Efficacy of electrical pulse mediated tomato lipophilic extract on human breast cancer cell. Journal of cancer research and therapeutics, 19(Supplement).

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Phytochemical Extraction of S. kunthianum

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The stem bark of S. kunthianum plant was collected from around Bonaya kebele, Wayu Tuka district, East Wollega Zone, Ethiopia and air dried. The herbarium specimen was identi ed by Dr.Tena Regasa at Biology Department, Wollega University, Ethiopia; where a voucher specimen was deposited for reference. Three solvents were used for polarity gradient extraction of stem bark of the S.kunthainum using conventional extraction technique, i.e. maceration, starting from petroleum ether, ethyl acetate, and methanol. About 350 grams of ne powder of stem bark of S.kunthainum was placed in 2000 mL conical ask and extracted with 1500 ml of Petroleum ether for 48 hours while shaking. Following this solvent extraction supernatant was ltered with lter paper (Whatman no 1 lter paper,) into ltrate and marc. The ltrate was kept for concentration but the Marc was allowed to dry at room temperature and the process of extraction was repeated with Ethyl acetate and methanol in the same manner successively.
Solvents were removed from the ltrate by rotary evaporator (rotovap) (Buchi R-210 Rotavapor System) under reduced pressure and crude extracts were collected separately and made to dry at room temperature and the dried masses were weighed and the percentage yield was calculated and stored in refrigerator below 4°C [10] , until used for preliminary qualitative phytochemical analysis and microbial assay.

Ameja L.B., Ayana M.T., Derese Z.B., Tariku Y., Yihun F.A., Kassa M.T., & Bahiru L.A. (2022). Qualitative phytochemical exploration guided in vitro antibacterial activity studies on crude stem bark extract of stereospermum kunthainum (Bignoniaceae).

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