Phalloidin conjugate to texas red

RPMI, DMEM, fetal bovine serum (FBS), Penicillin/Streptomycin (P/S), and trypsin–EDTA were purchased from Thermofisher (Grand Island, NY). Phalloidin-conjugate to Texas red and anti-fade with DAPI were obtained from Thermo Fisher (Eugene, OR). Purified mouse IgG_2B and IgG₁ myeloma protein were purchased from Cappel Pharmaceuticals, Inc. (Aurora, OH). DAPI, anti-rabbit, and anti-mouse conjugated to Alexa were from Thermo-Fisher (Eugene, OR). The in-situ cell death detection kit (TUNEL) was from Roche (Mannheim, Germany). siRNA was designed and synthesized by Origene. All protocols were evaluated and approved by UTMB.

All reagents were purchased from Sigma (St. Louis, MO), except in the places that are indicated otherwise. HIV_ADA was from the NIH AIDS Research and Reference Reagent Program (Germantown, MD). RPMI, fetal bovine serum (FBS), penicillin/streptomycin (P/S) and trypsin-EDTA were from Thermofisher (Grand Island, NY). Phalloidin-conjugate to Texas red and anti-fade with DAPI were obtained from Thermo Fisher (Eugene, OR). The HIV-p24 antibody was from Genetex (Irvine, CA). Purified mouse IgG_2B and IgG₁ myeloma protein were from Cappel Pharmaceuticals, Inc. (Aurora, OH). All protocols were evaluated and approved by Rutgers University. Human tissues were part of an ongoing research protocol approved by Rutgers University (IRB protocols Pro2012001303 and Pro20140000794).