K2edta

Manufactured by BD

Sourced in United States, United Kingdom, Germany

K2EDTA is a laboratory reagent used as an anticoagulant in blood collection tubes. It functions by binding to calcium ions, preventing the blood from clotting.

Automatically generated - may contain errors

Lab products found in correlation

Vacutainer tube, by BD (10 mentions) Microtainer tube, by BD (7 mentions) Vacutainer, by BD (5 mentions) Cell free dna bct, by Streck (5 mentions) Advia 2120, by Siemens (4 mentions) Rnalater, by Thermo Fisher Scientific (4 mentions) Sepmate tube, by STEMCELL (3 mentions) Microtainer blood collection tube, by BD (3 mentions) Countess 2, by Thermo Fisher Scientific (3 mentions) Hemavet hv950, by Drew Scientific (3 mentions) Ficoll paque plus, by GE Healthcare (2 mentions) Qiaamp circulating nucleic acid kit, by Qiagen (2 mentions) Nanodrop spectrophotometer, by Thermo Fisher Scientific (2 mentions) Mouse igg2a fitc, by BioLegend (2 mentions) Mouse igg1k pe, by BioLegend (2 mentions) Cd14 selection beads, by Miltenyi Biotec (2 mentions) Ultra sensitive mouse insulin elisa kit, by Crystal Chem (2 mentions) Vacutainer plus blood collection tubes, by BD (2 mentions) Au480 analyzer, by Beckman Coulter (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

K2EDTA MAP tubes K2EDTA coated microtainer tubes K2EDTA vacutainer blood collection tubes K2EDTA microtainers K2EDTA-coated vacutainers K2EDTA vacutainers BD Vacutainer® Plus Plastic K2EDTA Tubes K2EDTA-treated tubes BD Vacutainer® Tubes with K2EDTA K2EDTA plasma tubes

197 protocols using k2edta

Blood Sampling and Analysis in Dairy Cows

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples were collected via the jugular catheter from all cows at 0600 h on d 3 and 4 of P1, and on d 1, 3, 5, and 7 of P2 for plasma analysis. Blood samples were collected in tubes containing K 2 EDTA (Becton, Dickinson and Co.) and were centrifuged at 1,500 × g for 15 min at 4°C before being aliquoted to micro-centrifuge tubes and frozen at -20°C until analysis. Additionally, a 3-mL blood sample (K 2 EDTA; Becton, Dickinson and Co.) was obtained at 0600 h from d 2 to 4 of P1 and daily during P2 for complete blood count (CBC) analysis. Samples were obtained via the jugular catheter and stored at 4°C for ~3 h before submitting to the Iowa State University's Department of Veterinary Pathology for analysis.
Plasma insulin, nonesterified fatty acids (NEFA), BHB, BUN, glucose, LPS-binding protein (LBP), and serum amyloid A (SAA) concentrations were determined using commercially available kits according to manufacturers' instructions (insulin, Mercodia AB; NEFA, Wako Chemicals USA; BHB, Pointe Scientific Inc.; BUN, Teco Diagnostics; glucose, Wako Chemicals USA; LBP, Hycult Biotech; SAA, Tridelta Development Ltd.). The intraassay coefficients of variation for insulin, NEFA, BHB, BUN, glucose, LBP, and SAA were 6. 6, 4.8, 4.4, 5.9, 4.5, 6.3, and 4 .6%, respectively. The interassay coefficients for NEFA, BHB, BUN, and glucose were 10.1, 7.6, 16.1, and 5.3%, respectively.

Abeyta M.A., Horst E.A., Goetz B.M., Rodriguez-Jimenez S., Mayorga E.J., Al-Qaisi M, & Baumgard L.H. (2023). Effects of hindgut acidosis on inflammation, metabolism, and productivity in lactating dairy cows fed a high-fiber diet. Journal of dairy science, 106(4).

+ Open protocol

+ Expand

Sickle Cell Disease RBC Poloxamer 188

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole blood was collected from SCD patients with the HbSS genotype (n = 7 female, n = 6 male; 1–20 years of age; HbS = 21.6–85.9%; HbF = 0.7–32.9%, HbA = 0–66.3%) during their regular clinic visits to the Texas Children’s Hospital (Houston, TX) into 3 mL vacutainer tubes (K₂EDTA, BD), under a Baylor College of Medicine Institutional Review Board approved protocol. The %HbS, %HbF, %HbA, genotype, treatment type, gender, and age were obtained by review of patient’s medical records (see Supplementary Information for detailed patient information). Whole blood from normal individuals was collected from consenting volunteers into 4 mL vacutainer tubes (K₂EDTA, BD), under a University of Houston Institutional Review Board approved protocol. Blood samples from both healthy volunteers and SCD patients were leukocyte- and platelet-reduced by removing the buffy coat and platelet-rich plasma after gravity sedimentation at RT, and the RBCs were then re-suspended in normal saline at either 25% or 40% hematocrit (HCT). For Poloxamer 188 (P188) experiments, RBCs were incubated with 5 or 10 mg/mL P188 (Kolliphor P188; CAS: 9003-11-6; Sigma-Aldrich, St. Louis, MO) at 25% HCT for 30 min at 37°C, following a previously published protocol (Sandor et al., 2016 (link)).

Lu M., Kanne C.K., Reddington R.C., Lezzar D.L., Sheehan V.A, & Shevkoplyas S.S. (2021). Concurrent Assessment of Deformability and Adhesiveness of Sickle Red Blood Cells by Measuring Perfusion of an Adhesive Artificial Microvascular Network. Frontiers in Physiology, 12, 633080.

+ Open protocol

+ Expand

PRRSV Viremia and Antibody Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

For evaluation of viremia and PRRSV specific neutralizing antibody response, 5–7 mL of blood was collected at 0, 14, 26, 42 dpv and 3, 7, 10, 14 dpc. Plasma was separated using K2 EDTA plus blood collection tubes (BD vacutainer, NJ, USA) and preserved at −80 °C until use in the assays. At 26 dpv and 14 dpc, peripheral blood mononuclear cells (PBMC) were isolated from blood collected in EDTA by gradient centrifugation using lymphoprep solution in sepmate tubes (Stem Cell Technologies, Canada) [32 (link)]. PBMC were resuspended in enriched-RPMI (E-RPMI, RPMI containing 10 % FBS, 200 μm HEPES, 1 mM sodium pyruvate, 25 μm 2-ME, 1× Non-Essential Amino Acid, and 1× antibiotic and antifungal). Bronchoalveolar lavage (BAL) fluid was collected during necropsy as described previously [33 ] and aliquots were stored at −80 °C until use in the assays.

Ouyang K., Hiremath J., Binjawadagi B., Shyu D.L., Dhakal S., Arcos J., Schleappi R., Holman L., Roof M., Torrelles J.B, & Renukaradhya G.J. (2016). Comparative analysis of routes of immunization of a live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in a heterologous virus challenge study. Veterinary Research, 47, 45.

+ Open protocol

+ Expand

Blood Collection Protocol for Baseline and Donation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Prior to each blood collection, subjects were seated with their feet on the floor for a minimum of ten minutes per World Anti-Doping Agency blood collection guidelines (https://www.wada-ama.org/en/resources/world-anti-doping-program/guidelines-blood-sample-collection). After the ten-minute equilibration period, blood was collected via venipuncture of an antecubital vein into one 6 mL serum-separator tube and one 6 mL K₂EDTA (BD Vacutainer) tube. After collection, whole blood samples were immediately refrigerated until analysis. Additional aliquots were stored at −80C for HbA1c measurement. Following three baseline collections over the course of 2-4 weeks, each subject in the study donated one unit of blood (~475 mL) according to Associated Regional and University Pathologists (ARUP) standard operating procedures.

Chaudhury A., Miller G.D., Eichner D, & Higgins J.M. (2019). Single-cell modeling of routine clinical blood tests reveals transient dynamics of human response to blood loss. eLife, 8, e48590.

+ Open protocol

+ Expand

Analytical Sensitivity of CTC Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

To further verify the analytical sensitivity of the assay, we collected peripheral blood samples from two healthy donors in 2 different types of tubes: (a) K2EDTA (BD Vacutainer) and (b) CellSave (Menarini Silicon Biosystems). In both cases, the first 5 mL of blood was discarded to avoid skin epithelial cell contamination. In each tube, MCF‐7 cells (100 cells enumerated in a Malassez Hemocytometer) were spiked into peripheral blood (10 mL in EDTA tube and 7.5 mL in CellSave tube), and mixed immediately after spiking. In the EDTA tube, CTC isolation was performed by adding red cell lysis buffer and capture beads, coated with the monoclonal antibody BerEP4 against the human epithelial antigen EpCAM. In the CellSave tube, CellSearch^® analysis was performed according to the manufacturer's instructions. Following CTC analysis, CellSearch^® cartridges were used for downstream analysis of EpCAM‐positive CTCs. In both cases, DNA was isolated using QIAamp DNA Micro Kit and DNA concentration was measured in a NanoDrop 1000 spectrophotometer that was calibrated with the recommended CF‐1 standard solution.

Tzanikou E., Markou A., Politaki E., Koutsopoulos A., Psyrri A., Mavroudis D., Georgoulias V, & Lianidou E. (2019). PIK3CA hotspot mutations in circulating tumor cells and paired circulating tumor DNA in breast cancer: a direct comparison study. Molecular Oncology, 13(12), 2515-2530.

+ Open protocol

+ Expand

Blood Sampling and Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The patients were instructed not to eat for 12 hours before sample collection. Blood samples (20 mL) were obtained in the morning (not later than 8 AM) by venous puncture. Four milliliters were transferred to glass tubes containing 7.2-mg K2-ethylenediaminetetraacetic acid (K2-EDTA; BD Vacutainer, Franklin Lakes, NJ, USA) for glycated hemoglobin and blood count. Another 4 mL were transferred to glass tubes with 6 mg of NaF and 12 mg Na₂EDTA (BD Vacutainer) for glucose analysis. Eight milliliters were transferred to clot activator glass tubes (BD Vacutainer) for lipid profile, high-sensitivity C-reactive protein, insulin and ω-3 and ω-6 LC-PUFA (EPA, DHA, DPA, and AA). All of the samples were immediately centrifuged for 5 minutes, except the K2-EDTA, and used for immediate analysis or stored at -70℃. Blood collection was carried out at T0, T1, and T2.

Martinez G.L., Koury J.C., Martins M.A., Nogueira F., Fischer R.G., Gustafsson A, & Figueredo C.M. (2014). Serum level changes of long chain-polyunsaturated fatty acids in patients undergoing periodontal therapy combined with one year of omega-3 supplementation: a pilot randomized clinical trial. Journal of Periodontal & Implant Science, 44(4), 169-177.

+ Open protocol

+ Expand

Plasma Biochemical and Hormonal Profiles in Laying Hens

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ten laying hens from each group were randomly chosen at the end of the experiment, and blood samples were collected from the wing vein in K₂-EDTA heparinized tubes (K₂-EDTA, BD Vacutainer, Plymouth, Devon, UK). The blood samples were centrifuged at 3,000 rpm for 15 min at 4°C to separate the plasma into Eppendorf tubes and kept at −20°C until further analysis. The concentrations of ALB, HDL, Ca, P, total protein (TP), glucose (GLU), triglycerides (TG), total cholesterol (TC), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were determined by an automatic blood biochemistry analyzer (Konelab 20 analyzer, Thermo Fisher Scientific, Vantaa, Finland), using commercial diagnostic kits and following the manufacturer's guidelines. Besides, plasma FSH, LH, and E2β were determined using a homologous radioimmunoassay (Krishnan et al., 1993 ).

Eltahan H.M., Cho S., Rana M.M., Saleh A.A., Elkomy A.E., Wadaan M.A., Alagawany M., Kim I.H, & Eltahan H.M. (2023). Dietary exogenous phytase improve egg quality, reproductive hormones, and prolongs the lifetime of the aging Hy-Line brown laying hens fed nonphytate phosphorus. Poultry Science, 102(9), 102895.

+ Open protocol

+ Expand

CTC Isolation from Whole Blood

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

PB was collected before the treatment began (baseline) and IMV was collected during surgery in order to conduct CTC evaluation. Samples were kept in plastic blood collection tubes with K2EDTA (BD Vacutainer^®, Plymouth, UK) at room temperature and were processed within 24 h of collection. The isolation procedure began with the Leucosep TM tube (bio-check LABORATORIES LTD, New Taipei City, Taiwan) to remove red blood cells from whole blood. In this step, red blood cells were removed through Ficoll–Paque PLUS (GE Healthcare Life Sciences, Taipei, Taiwan) by concentration gradient. The mononucleated cells containing CTC (peripheral blood mononuclear cells (PBMCs)) after centrifugation were collected, and the CTC cell retention rate was about 75–85% [30 (link)].

Chu H.Y., Lu L.S., Cho W., Wu S.Y., Chang Y.C., Lin C.P., Yang C.Y., Lin C.H., Jiang J.K, & Tseng F.G. (2019). Enumerating Circulating Tumor Cells with a Self-Assembled Cell Array (SACA) Chip: A Feasibility Study in Patients with Colorectal Cancer. Cancers, 11(1), 56.

+ Open protocol

+ Expand

Plasma ctDNA Extraction from Whole Blood

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole blood samples were collected into venous blood collection tubes using K₂EDTA (BD Vacutainer, Plymouth) as anticoagulant. Samples were mixed thoroughly, and plasma was isolated within 2 h from sample collection by centrifugation at 530 g for 10 min at room temperature. Once isolated, plasma samples were further centrifuged twice at 2000 g for 10 min, before transferring into clean 2‐mL tubes and freezing at −70 °C until further processing. The QIAamp Circulating Nucleic Acid Kit (Qiagen, Hilden, Germany) was used to isolate ctDNA from 2.00 mL of plasma according to manufacturer's instructions.

+ Open protocol

+ Expand

Isolation and Quantification of PBMC-Derived DNA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples (~0.7 mL) were obtained by intracardiac puncture and were mixed with 3 mL of PBS (pH 7.4) in a blood collection tube spray-coated with K₂EDTA (BD Vacutainer). This mixture was then carefully layered over 4 mL of Histopaque 1083 (Sigma-Aldrich) solution in 15-mL conical tubes. The samples were then centrifuged at 18°C–20°C for 30 minutes (400 g; deceleration without brake), and the layer containing PBMCs was carefully pipetted out into 1-mL Eppendorf tubes. After subsequent washes with PBS (2 × 10 minutes), centrifugation (2 × 10 minutes at 400 g), and removal of supernatant solution, the PBMC pellet was processed further for DNA isolation using the DNeasy Blood and Tissue kit (Qiagen) per the manufacturer’s instructions. The quantity and quality of DNA were quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific).

Muralidharan A., Sotocinal S.G., Yousefpour N., Akkurt N., Lima L.V., Tansley S., Parisien M., Wang C., Austin J.S., Ham B., Dutra G.M., Rousseau P., Maldonado-Bouchard S., Clark T., Rosen S.F., Majeed M.R., Silva O., Nejade R., Li X., Donayre Pimentel S., Nielsen C.S., Neely G.G., Autexier C., Diatchenko L., Ribeiro-da-Silva A, & Mogil J.S. (2022). Long-term male-specific chronic pain via telomere- and p53‑mediated spinal cord cellular senescence. The Journal of Clinical Investigation, 132(8), e151817.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!