Sigmastat

All statistical analyses were performed using SigmaStat (SPSS Inc., Chicago, IL) with an error rate set at α = 0.05. The significance difference between treatments was tested using Tukey's Honestly Significant Difference Test.

Data were analyzed offline using software from Spike 2 (CED, Cambridge, UK). LTP was expressed as the PSP slope and normalized to the average PSP slope over the last 10 min before LTP induction.
All statistical tests were performed using SigmaStat software (SPSS Inc., California, USA). Data sets were tested for normality using the Shapiro–Wilk test, but no deviation from a normal distribution was detected and results are expressed as mean ± standard error of mean. Comparison of HFS‐induced plasticity was performed between groups using two‐way repeated‐measures ANOVA. This analysis was followed by a post hoc t‐test performed between groups. Differences between groups and changes within groups were considered statistically significant if P < 0.05.

A power analysis powered for a two-tailed t-test was carried out prior to subject enrollment to determine the minimum required sample sizes needed to detect a 1.5-fold (assumed) statistically significant difference in growth factor between cases (PCOS) and controls. The required level of significance was at least 95% (p = 0.05), and the required power of the test was at least 90% (p = 0.10). Based on this calculation the required sample sizes were n = 14 for each group. Data were analyzed by Student’s t-test or the Mann–Whitney test, as appropriate. Results are expressed as mean +/- standard error of the mean (SEM). Correlations between PlGF or sFlt-1 and various parameters were performed using Pearson correlation tests. SigmaStat (SPSS Science, Chicago, IL) was used for statistical analysis. All significance tests were two-tailed and P-value <0.05 was considered to be statistically significant.

All statistical analysis was performed using SigmaStat (version 13, SPSS, Chicago, IL). Data are presented as means ± SEM for both absolute values and percent change relative to individual baseline. Any data points that were more than 2 standard deviations from the mean values were designated as outliers and removed from analysis. For multiple group comparison, we used repeat measures 2-way ANOVA with the Holm-Sidak method post hoc analysis where appropriate. Values of P ≤ 0.05 were considered statistically significant. All plots were created using Prism (GraphPad, San Diego, CA).

All data were analyzed, using commercial software (SigmaStat, SPSS, Chicago, IL). All outcomes were analyzed using a two‐way ANOVA (genotype x diet), except for PPARγ western blot analysis, which was analyzed by a Two‐Tailed Student's t‐Test. Student–Newman–Keuls test was used for all post hoc analyses. Any data that were not normally distributed or did not display equal variance were logarithmically transformed so that these criterion were met. Statistical significance was set with an alpha value of P < 0.05. Data are presented as mean (±SEM).