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4 protocols using dimethyl adipate

1

Polymer Synthesis and Characterization

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Adipic acid (>99%), succinic acid (>99%), dodecanedioic acid (>99%), dimethyl adipate (>99%), titanium butoxide (>99%), ethylene glycol (>99.8), PCL homopolymers with molar masses of 14,000 and 80,000 g/mol were purchased from Sigma Aldrich (Steinheim, Germany). All the other products and reagents were used without further purification.
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2

Synthesis of Polyester Precursors using Polypropylene Beads

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Polypropylene beads (Accurell MP1000 surface area of 55,985 m2 g−1, particle density of 1.993 cm−3, and particle diameter of <1500 mm) were purchased from 3M Deutschland GmbH (Wuppertal, Germany). Dimethyl adipate (DMA, >99%), dimethyl sebacate (DMSe, >99%), 1,4-butanediol (BDO, >99%), 1,8-octanediol (ODO, >98%), butyric acid (>99%), 1-butanol (>99%), 1-octanol (>99%), 1-dodecanol (>99%), lauric acid (>99%), 2-methyl-2-butanol (>99%), 4-nitrophenol (>99%), para-nitrophenyl butyrate (P-NPB), Na2HPO4 (>98%), NaH2PO4 (>98%), and toluene (>99.8%) were purchased from Sigma-Aldrich. Dimethyl itaconate (DMI, 98%) was purchased from TCI chemicals (Vienna, Austria). Octanoic acid (>98%) and 2-methyltetrahydrofuran (MeTHF, >99%) were purchased from Alfa Aesar (Kandel, Germany). Chloroform (for HPLC, >99.8%) was purchased from VWR Chemicals (Wien, Austria).
All chemicals and solvents were used as received if not otherwise specified.
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3

Enzymatic Synthesis of Dicarboxylic Esters

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Glycerol (99.5 %) provided by Carlo Erba e da A.C.E.F.. Diethyl malonate (99 %), dimethyl adipate (98 %), dimethyl pimelate (99 %), dimethyl suberate (99 %), diethyl azelate (90 %), diethyl sebacate (98 %) were purchased from Sigma‐Aldrich. CaLB was covalently immobilized on epoxy‐methacrylic resins Relizyme EP 113, kindly donated by Resindion (Mitsubishi Chemical Corporation), Binasco, Italy. The specific hydrolytic activity determined based on previously reported protocol was 350 U/g. Azelaic acid was kindly provided by Novamont S.p.A, Novara, Italy. All reagents were used without further purification
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4

Solvent Extraction Optimized Protocol

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All analytical standards, with certified purity > 99%, of Di-methyladipate (DMA), di- n-hexane (99%), Na 2 SO 4 (anhydrous, ACS grade), and the pre-packaged 15 ml centrifuge tube with 900 mg MgSO 4 and 150 mg PSA (an ethylenediamine-N-propyl phas) were bought from Sigma-Aldrich (Steinheim, Germany).
During samples preparation and analysis, laboratory glassware heated at 400 °C for at least 4 h were used (Fankhauser and Grob, 2007) always, while laboratory gloves were never used.
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