Specific kits against IL-10 (Boster Bio, EK0417) and TGF-β (Boster Bio, EK0515) were used to detect cytokine levels before and after HSK recurrence in serum and spleen cells of mice. The procedure was performed according to the manufacturer’s instructions.
Ek0417
Manufactured by Boster Bio
Sourced in United States, China
The EK0417 is a laboratory equipment product. It is designed to perform a core function within the laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using ek0417
1
Cytokine Measurement in HSK Recurrence
2
Serum Cytokine and Growth Factor Quantification
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The levels of IL-4 (EK0405, Boster, Pleasanton, CA, USA), IL-9 (ABIN625153, RayBiotech, Norcross, GA, USA), IL-10 (EK0417, Boster), IL-25 (ABIN425028, Cloud-Clone, Houston, TX, USA), VEGF (ABIN2116393, antibodies-online, Germany), and TGF-β (ABIN772539, Blue Gene, San Francisco, CA, USA) in serum were determined by ELISA kits according to the manufacturer’s instructions.
3
Quantifying IL-10 in Colon Tissue
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For extracting total protein, the liver was homogenized in a mixture of RIPA buffer (P0013C, Beyotime, Shanghai, China), 2% phosphatase inhibitor (P1081, Beyotime, Shanghai, China) and 1% phenylmethanesulfonyl fluoride (PMSF, ST506, Beyotime, Shanghai, China). Following centrifugation at 14,000 r/min for 15 minutes at 4°C, supernatants were collected. The level of il-10 (EK0417, Boster, Wuhan, China) in colon was quantified by using commercial ELISA kit according to the manufacturer’s instructions. The detection limit of the kit is 15.6-1000 pg/ml for il-10.
4
Hippocampal Cytokine Profiling
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The levels of tumor necrosis factor-alpha (TNF-α) (Boster Biological Technology, Wuhan, China, EK0527), interleukin-1 beta (IL-1β) (Boster, EK0394) and IL-10 (Boster, EK0417) in the hippocampus were detected by an ELISA kit in accordance with the manufacturer’s instructions and represented as pg/mL.
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