CiSCs, BM-MSCs, MCF7 cells, and Hela cells were plated in a 12-well plate at a density of 4 × 105 cells per well. Cells were then treated with cisplatin (at concentrations of 5, 10, 15, 20, and 25 μM) or doxorubicin (2, 6, and 10 nM). After incubation for 24 hours, the viability and apoptosis induced by anticancer regimens was analyzed by flow cytometry using an Annexin-V-FITC and propidium iodide (PI) apoptosis detection kit (Miltenyi Biotec Inc., USA) according to the manufacturer’s protocol. Experiments were performed three times in triplicate each.
Annexin 5 fitc and propidium iodide apoptosis detection kit
Manufactured by Miltenyi Biotec
Sourced in United States
The Annexin-V-FITC and propidium iodide (PI) apoptosis detection kit is a laboratory tool used to identify and quantify apoptotic cells. It combines the application of fluorescently-labeled Annexin V, which binds to phosphatidylserine, and the DNA-binding dye propidium iodide to distinguish between early apoptotic, late apoptotic, and necrotic cells.
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Lab products found in correlation
2 protocols using annexin 5 fitc and propidium iodide apoptosis detection kit
1
Evaluating Anticancer Regimen Cytotoxicity
2
Evaluating Anti-Cancer Drug Cytotoxicity
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Cells were plated in a 12-well plate at a density of 4×105 cells/well. Cells were then treated with cisplatin at concentrations of (5, 10, 15, 20 and 25 µM) or Doxorubicin (2, 6 and 10 nM). After incubation for 24 h, the viability and apoptosis induced by anticancer regimens were analyzed by flow cytometry using an Annexin-V-FITC and propidium iodide (PI) apoptosis detection kit (Miltenyi Biotec Inc., Auburn, CA, USA) as per the manufacturer's protocol. Experiments were each performed three times in triplicate.
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