Protran premium membrane

Manufactured by GE Healthcare

Protran Premium membrane is a product offered by GE Healthcare. It is a laboratory equipment designed for scientific applications. The core function of the Protran Premium membrane is to facilitate sample separation and analysis processes in research and diagnostic settings.

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Lab products found in correlation

Odyssey imaging system, by LI COR (2 mentions) Irdye 800cw conjugated antibody, by LI COR (2 mentions) Nupage novex bis tris gel 1.0 mm, by Thermo Fisher Scientific (2 mentions) Odyssey application software, by LI COR (1 mentions)

2 protocols using protran premium membrane

Western Blot Analysis of Serum Proteins

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Protein samples were separated by SDS-PAGE electrophoresis (4–12% gradient, NuPAGE Novex Bis-Tris Gel 1.0 mm, Life Technologies) and transferred onto Protran Premium membrane (nitrocellulose, GE Healthcare). Fifty micrograms (1 µl of serum) of human, mouse or dog serum protein were loaded per lane. Antibodies against MYOM3 (1:1000, Proteintech: 17692-1-AP) and the CK-M (1:500, Santa Cruz: sc-15161) were used as primary antibodies followed by incubation with the corresponding IRDye-800CW-conjugated antibodies (1:10 000, LI-COR Biosciences) according to the manufacturer's instructions. Infrared fluorescence of the secondary antibodies was read on an Odyssey Imaging System (LI-COR Biosciences). Band intensities were measured by the Odyssey application software (LI-COR Biosciences, Image Studio Lite 4.0 Version). The following antibodies were not efficient in Western blot analysis of human serum: anti-Myomesin 3 sc-165061; anti-Myomesin 2: sc-30384; sc-30385; sc-50435; anti-Myosin: ABIN502241; ABIN616957; sc-53089; sc-20641; sc-32732; sc-53096.

Rouillon J., Poupiot J., Zocevic A., Amor F., Léger T., Garcia C., Camadro J.M., Wong B., Pinilla R., Cosette J., Coenen-Stass A.M., Mcclorey G., Roberts T.C., Wood M.J., Servais L., Udd B., Voit T., Richard I, & Svinartchouk F. (2015). Serum proteomic profiling reveals fragments of MYOM3 as potential biomarkers for monitoring the outcome of therapeutic interventions in muscular dystrophies. Human Molecular Genetics, 24(17), 4916-4932.

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Ferritin Protein Detection by Western Blot

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Protein samples were separated by SDS-PAGE electrophoresis (4%-12% gradient, NuPAGE Novex Bis-Tris Gel 1.0 mm, Life Technologies) and transferred onto Protran Premium membrane (nitrocellulose, GE Healthcare). Antibodies against Ferritin (1:1000, Genetex: GTX-62019) were used as primary antibodies followed by incubation with the corresponding IRDye-800CW-conjugated antibodies (1:10 000, LI-COR Biosciences) according to the manufacturer's instructions. Infrared fluorescence of the secondary antibodies was read on an Odyssey Imaging System (LI-COR Biosciences). Band intensities were measured by the Odyssey application software (LI-COR Biosciences, Image Studio Lite 4.0 Version).

Rouillon J., Lefebvre T., Denard J., Puy V., Daher R., Ausseil J., Zocevic A., Fogel P., Peoc'h K., Wong B., Servais L., Voit T., Puy H., Karim Z, & Svinartchouk F. (2018). High urinary ferritin reflects myoglobin iron evacuation in DMD patients. Neuromuscular disorders : NMD, 28(7).

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