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The B2615 is a laboratory centrifuge designed for general-purpose applications. It features a compact and durable construction, with a maximum speed of 6,000 rpm and a maximum capacity of 6 x 50 mL. The centrifuge is suitable for separating and concentrating a variety of samples, including biological samples, cell cultures, and microbiological specimens.

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Lab products found in correlation

F1804, by Merck Group (2 mentions) A0173, by ABclonal (2 mentions) Ac002, by ABclonal (2 mentions) Ecl chemiluminescent substrate, by GE Healthcare (1 mentions) Pvdf membrane, by GE Healthcare (1 mentions) Gtx125926, by GeneTex (1 mentions) Gtx125989, by GeneTex (1 mentions) Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (1 mentions) Goat anti mouse igg hrp, by Thermo Fisher Scientific (1 mentions) Gtx125928, by GeneTex (1 mentions) Gtx125932, by GeneTex (1 mentions) Gtx125923, by GeneTex (1 mentions) Polyvinylidene fluoride membrane, by GE Healthcare (1 mentions) A12737, by ABclonal (1 mentions) Ae003, by ABclonal (1 mentions)

2 protocols using b2615

1

Western Blot Analysis of Influenza Proteins

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Cells were lysed using 4 ​× ​SDS loading buffer and denatured at 95 ​°C for 10 ​min. Protein samples were resolved by SDS-PAGE, transferred to PVDF membranes (GE Healthcare), and processed for western blotting. Western blot detection of MYH9 was performed using a rabbit anti-MYH9 antibody (1:1,000, A0173, Abclonal), with a goat anti-rabbit IgG-HRP antibody (1:3,000, B2615, Santa Cruz Biotechnology) as the secondary antibody. Other antibodies used in the study included: mouse anti-Flag M2 (1:2,000, F1804, Sigma), rabbit anti-Lamin B1 (D4Q4Z) mAb (1:3,000, 12,586, Cell Signaling Technology), mouse anti-GAPDH (1:3,000, AC002, Abclonal), rabbit anti-Influenza A virus NP antibody (1:2,000, GTX125989, GeneTex), rabbit anti-Influenza A virus M1 antibody (1:2,000, GTX125928, GeneTex), rabbit anti-Influenza A virus PA antibody (1:2,000, GTX125932, GeneTex), rabbit anti-Influenza A virus PB1 antibody (1:2,000, GTX125923, GeneTex), rabbit anti-Influenza A virus PB2 antibody (1:2,000, GTX125926, GeneTex), goat anti-rabbit IgG-HRP (1:5,000, B2615, Santa Cruz Biotechnology), and goat anti-mouse IgG-HRP (1:5,000, 31,430, Invitrogen) antibodies. GAPDH was used as a loading control. Protein bands were visualized by either fluorescence or using a chemiluminescent ECL substrate (GE Healthcare).
Chen J., Liu J., Chen Z., Feng D., Zhu C., Fan J., Zhang S., Zhang X, & Xu J. (2022). Nonmuscle myosin IIA promotes the internalization of influenza A virus and regulates viral polymerase activity through interacting with nucleoprotein in human pulmonary cells. Virologica Sinica, 38(1), 128-141.
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2

Western Blot Analysis of MYH9 and ACE2

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Cells were lysed using 4×SDS loading buffer and denatured at 95 °C for 10 min. Protein samples were resolved by SDS-PAGE, transferred to polyvinylidene fluoride membranes (GE Healthcare), and processed for Western blotting. Western blot detection of MYH9 and ACE2 was performed using a rabbit anti-MYH9 antibody (1:1,000, A0173, Abclonal) and an anti-ACE2 antibody (1:1,000 dilution, A12737, Abclonal), with a goat anti-rabbit IgG-HRP antibody (1:3,000, B2615, Santa Cruz Biotechnology) as the secondary antibody. GAPDH was used as a loading control.
Other antibodies used in the study included: mouse anti-Flag M2 (1:2,000, F1804, Sigma-Aldrich), mouse anti His-tagged mAb (1:1,000, AE003, Abclonal), anti-GAPDH (1:3,000, AC002, Abclonal), goat anti-rabbit IgG-HRP (1:5,000, B2615, Santa Cruz Biotechnology), and goat anti-rabbit IgG-HRP (1:5,000, 31430, Invitrogen) antibodies.
Chen J., Fan J., Chen Z., Zhang M., Peng H., Liu J., Ding L., Liu M., Zhao C., Zhao P., Zhang S., Zhang X, & Xu J. (2021). Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells. Proceedings of the National Academy of Sciences of the United States of America, 118(50), e2111011118.
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