Diamat ion exchange hplc

Manufactured by Bio-Rad

Sourced in Germany, United Kingdom

The Diamat ion exchange HPLC is a high-performance liquid chromatography (HPLC) system designed for the separation and purification of ionic compounds. It utilizes ion exchange principles to facilitate the separation process. The system is capable of performing analytical and preparative ion exchange chromatography.

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Lab products found in correlation

Ra 1000, by Bayer (2 mentions) Ra1000 autoanalyzer, by Bayer (1 mentions)

Close spelling variants of this product

Diamat

3 protocols using diamat ion exchange hplc

Comprehensive Metabolic Health Assessment

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We examined changes in BMI, waist circumference, the ratio of total to HDL cholesterol, glycated hemoglobin (HbA1c), and blood pressure (systolic and diastolic) as primary outcomes. Changes in weight, waist-to-hip ratio, total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, and a metabolic-risk z score were examined as secondary outcomes. Weight, height, waist circumference, and hip circumference were measured with a standardized protocol. BMI was defined as weight divided by height squared. Blood pressure was measured twice (using the average) with an Accutorr sphygmomanometer (Datascope). Total cholesterol, HDL cholesterol, triglycerides (RA 1000; Bayer Diagnostics) and HbA1c (Diamat ion exchange HPLC; Bio-Rad Laboratories) were measured in nonfasting blood. LDL cholesterol was calculated using the Friedewald formula (20 (link)). The metabolic risk z score was calculated as previously described (21 (link)) by averaging z scores of blood pressure (systolic and diastolic), HbA1c, waist circumference, HDL cholesterol multiplied by −1, and log-transformed triglycerides.

Trichia E., Luben R., Khaw K.T., Wareham N.J., Imamura F, & Forouhi N.G. (2020). The associations of longitudinal changes in consumption of total and types of dairy products and markers of metabolic risk and adiposity: findings from the European Investigation into Cancer and Nutrition (EPIC)–Norfolk study, United Kingdom. The American Journal of Clinical Nutrition, 111(5), 1018-1026.

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Blood Lipids and Lipoprotein Analysis Protocol

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Nonfasting blood samples were taken by venipuncture in EDTA-containing vacutainers at the baseline clinic visit and processed at the Department of Clinical Biochemistry, University of Cambridge (UK), where all lipids assays were performed.^{41 (link)} Plasma was isolated by centrifugation for 15 minutes, 3000 rpm, 4 °C and stored at -80 °C for future analysis. Circulating levels of total cholesterol, HDL-C and TG were measured on fresh samples with the RA 1000 (Bayer Diagnostics, Basingstoke, United Kingdom) and LDL-C levels were calculated with the Friedewald formula.^{42 (link)} Non-HDL-cholesterol was calculated by subtracting HDL-C from total cholesterol. Apo B was measured by using rate immunonephelometry (Behring Nephelometer BNII, Marburg, Germany).^{43 (link)} HbA1c was measured using Diamat ion exchange HPLC (Bio-Rad Laboratories, Hemel Hempstead, United Kingdom).^{39 (link)}In the case-control subset additional parameters were tested. Lipoprotein particle size and particle number was analyzed with an automated nuclear magnetic resonance (NMR) spectroscopy by Liposcience Co as described.^{44 (link)} Plasma LPL mass and Apo A-V were measured as described.^{16 (link)} Samples were analyzed in random order to avoid systemic bias. Researchers and laboratory personnel had no access to identifiable information and could identify samples by number only.

Verbeek R., Oldoni F., Surendran R.P., Zwinderman A.H., Khaw K.T., Stroes E.S., Wareham N.J., Boekholdt S.M, & Dallinga-Thie G.M. (2019). A 3-SNP Gene Risk Score and a Metabolic Risk Score both Predict Hypertriglyceridemia and Cardiovascular Disease Risk. Journal of clinical lipidology, 13(3), 492-501.

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Cardiometabolic Risk Score Measurement

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Trained research staff measured height, weight and waist circumference according to standardised procedures (Hayat et al., 2014 (link)). Systolic and diastolic blood pressure were measured in duplicate using an Accutorr sphygmomanometer (Datascore, UK) after three minutes of sitting, with the average taken. The non-fasting venous blood sample was examined for serum triglycerides (TG), total cholesterol (TC), and high-density lipoprotein cholesterol (HDL-C) using an RA1000 autoanalyzer (Bayer Diagnostics, UK). Glycated haemoglobin (HbA1c) was measured using Diamat ion exchange HPLC (Bio-Rad Laboratories, UK). A continuously distributed CMR score was computed using continuous indicators of waist circumference; blood pressure (average of systolic and diastolic); TG; TC:HDL-C; and, HbA1c. After log-normalisation of TG, TC:HDL-C and HbA1c, all five cardiometabolic variables were standardised [z = (value-mean)/SD)] in sex-specific strata. The CMR score was then calculated by summing all sex-standardised scores and dividing this sum by five. A higher CMR score indicates higher cardiometabolic disease risk. We confirmed face validity of this CMR score in the present population by examining its prospective association with incident cardiovascular disease, the risk of which was estimated to be 47% higher per 1 unit in the CMR score in a linear manner (see Supplemental Analysis S1).

Dempsey P.C., Aadland E., Strain T., Kvalheim O.M., Westgate K., Lindsay T., Khaw K.T., Wareham N.J., Brage S, & Wijndaele K. (2022). Physical activity intensity profiles associated with cardiometabolic risk in middle-aged to older men and women. Preventive Medicine, 156, 106977.

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