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2 protocols using anti bap31

1

Protein Expression Analysis by Immunoblotting

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Protein analysis was performed using standard immune blotting. The following antibodies were used at the indicated dilution: anti-Noxa (SC-2697) 1:1000; anti-cytochrome (#4212), 1:1000; anti-caspase 3 (#7190), 1:1000; anti-caspase 9 (#9501), 1:1000; anti-PARP (#9542), 1:500 (each Cell Signaling Technology Inc., Danvers, MA, USA); anti-IDO antibody 1:500 (BioGenes, Berlin, Germany); anti-ASK1 (Sc-7931), 1:500; anti-p-ASK1 (Sc-109911), 1:1000; anti-JNK (Sc-474), 1:1000; anti-p-JNK (SC-6254), 1:1000; anti-p38 (Sc-535), 1:1000; anti-p-p38 (Sc-7973), 1:1000; anti-Actin (Sc-1615), 1:5.000; anti-Tom20 (Sc-11415), 1:100; anti-Bap31 (Sc-18579), 1:1000; anti-HO-1 (sc-10789), 1:1000; anti-p-JAK1 (sc-16773), 1:1000, anti-IRE1α (Sc-20790), 1:500; anti-PERK (SC-9477), 1:1000; (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA); anti-IκBα (Sc-7182), 1:1000; anti-p-IκBα (AF4809, R&D system), 1:1000; anti-JAK1 antibody (ab47435), 1:1000; anti-IRF1 antibody (ab55330), 1:1000 (each ABCAM).
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2

Antibody List for Protein Analysis

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The following antibodies were purchased from Cell Signaling Technology: anti-IRS-1 (2382), anti-pAKT S473 (9271), anti-p85 (4292), anti-IGF-1Rβ (9750), anti-PDK1 (13037). Other antibodies were from the following commercial sources: anti-VAPB (Sigma-Aldrich, HPA013144), anti-NOGOA (Bio-Rad, AHP1799), anti-BAP31 (Santa Cruz Biotechnology, sc-48766), anti-PERK (abcam, ab229912), anti-pPERK (Affinity Biosciences, DF7576), anti-AKT (HUABIO, ET1609-47), anti-Actin (HUABIO, M1210-2), anti-Tubulin (HUABIO, M1305-2), anti-GFP (HUABIO, ET1607-31), anti-FLAG (YEASEN, 30503ES60), anti-HA (Invitrogen, PA1-985) and anti-mCherry (ABclonal, AE002).
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