Rat specific elisa

To identify the changes in islets, one pancreas biopsy was performed on the day of diabetes manifestation, immediately before the start of therapy, and another at the end of therapy as described previously [35 (link)]. The remaining pancreas comprising head and tail was collected for analyses 60 days and after anti-TCR combination with anti-TNF-α also 360 days (group 6) after the end of therapy including serum samples. Blood glucose concentrations were determined daily (Glucometer Elite®, Bayer, Leverkusen, Germany). Serum C-peptides were analysed with a rat-specific ELISA (Mercodia, Uppsala, Sweden) and serum cytokine protein concentrations with a multiplex immunoassay kit (Bio-Rad, Munich, Germany) [15 (link), 16 (link)].

Tissue specimens were fixed for microscopic analyses [29 (link)]. Blood glucose concentrations were determined daily (Glucometer Elite®, Bayer, Leverkusen, Germany). Serum C-peptide was analysed with a rat-specific ELISA (Mercodia, Uppsala, Sweden), and serum cytokine protein concentrations with a multiplex immunoassay kit (Bio-Rad) [25 (link), 29 (link)].