The crystallinity of the samples was determined by X-ray diffraction (XRD) using a diffractometer with Cu Kα radiation (Shimadzu LabX XRD-6000). Grazing-incidence XRD (GIXRD) measurements were performed with D8 Discover with GADDS (Bruker AXS Gmbh, Karisruhe, Germany). Cross-sectional transmission electron microscopy (TEM) images were taken with a FEI Tecnai Osiris system and top-view TEM images were taken with a FEI Tecnai F20 system. The morphologies of the prepared samples were obtained using a field emission scanning electron microscopy (SEM) (Hitachi, S-8010). X-ray photoemission spectroscopy (XPS) was performed by a Ulvac-PHI 1600 spectrometer with monochromatic Al Kα X-ray radiation (1486.6 eV). Raman and photoluminescence (PL) spectroscopy were conducted with HORIBA LabRAM HR800 except for the Raman mapping. We used 632 nm excitation wavelength for the measurements. The Si peak at 520 cm−1 was used as a reference for wavenumber calibration. The Raman mapping was performed on Nanofinder 30 (Tokyo Instruments, Inc.).
Tecnai osiris system
Manufactured by Thermo Fisher Scientific
The Tecnai Osiris system is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of materials at the nanoscale. It provides advanced capabilities for structural and chemical characterization of a wide range of samples.
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Lab products found in correlation
3 protocols using tecnai osiris system
1
Comprehensive Characterization of Nanomaterials
2
Synthesis and Characterization of MK2i and p-HSP20 Nanoparticles
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PPAA was dissolved in 1 M NaOH and diluted into a phosphate buffer (pH 8) to obtain a stock solution. Purified MK2i peptide or p-HSP20 peptide was dissolved in phosphate buffer (pH 8). The MK2i peptide or p-HSP20 peptide was then mixed with the PPAA polymer at a range of CRs from [NH3+]/[COO–] = 10:1 to 1:10 to form MK2i-NPs or p-HSP20-NPs, respectively. The resulting nano-polyplexes were syringe-filtered through a 0.45 μm PTFE (polytetrafluorethylene) filter, and the hydrodynamic diameter and ζ-potential were characterized on a Malvern Zetasizer Nano-ZS with a reusable dip cell kit (Malvern Instruments Ltd., Worcestershire, U.K.).
A CR of 1:3 was chosen as the lead MK2i-NP formulation, whereas a charge ratio of 3:1 was chosen as the lead p-HSP20-NP formulation; these formulations were used in subsequent in vitro and ex vivo experiments. In order to verify the sizes indicated by DLS analysis, optimized MK2i-NP and HSP20-NP formulations were visualized through transmission electron microscopy imaging. TEM samples were prepared by inverting carbon-film-backed copper grids (Ted Pella) onto a 20 μL droplet of aqueous polyplex suspensions (1 mg/mL) and blotted dry. All samples were then inverted onto a 20 μL droplet of 3% uranyl acetate and stained for 30 s. Samples were then desiccated in vacuo for 2 h prior to imaging on a FEI Tecnai Osiris system.
A CR of 1:3 was chosen as the lead MK2i-NP formulation, whereas a charge ratio of 3:1 was chosen as the lead p-HSP20-NP formulation; these formulations were used in subsequent in vitro and ex vivo experiments. In order to verify the sizes indicated by DLS analysis, optimized MK2i-NP and HSP20-NP formulations were visualized through transmission electron microscopy imaging. TEM samples were prepared by inverting carbon-film-backed copper grids (Ted Pella) onto a 20 μL droplet of aqueous polyplex suspensions (1 mg/mL) and blotted dry. All samples were then inverted onto a 20 μL droplet of 3% uranyl acetate and stained for 30 s. Samples were then desiccated in vacuo for 2 h prior to imaging on a FEI Tecnai Osiris system.
3
TEM-EDS Chemical Mapping Protocol
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As prepared TEM sections are analyzed using an FEI Tecnai Osiris™ system at 200 kV accelerating voltage. Four windowless silicon drift detectors placed at 90°with respect to each other are used to obtain the chemical maps. A 1 nA beam is used in a ~512 × 512 array with a 25 µs dwell time for ~10 min. The EDS data is then quantified and deconvoluted using the Cliff-Lorimer method.
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