Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples using a Ficoll gradient (Axis Shield, Norway). Unstimulated PBMCs were stained with APC anti-human CD19 antibody (BioLegend cat #302212), APC/Cyanine7 anti-human CD138 antibody (BioLegend cat #356528), PerCP/Cyanine5.5 anti-human CD1d antibody (BioLegend cat #350312), APC anti-human CD25 antibody (BioLegend cat #302610), and PE anti-human CD223 (LAG-3) antibody (BioLegend cat #369306). After surface staining, cells were stained with PE anti-human EBi3 antibody (BioLegend cat #360904) and PE/Cy7 anti-human IL-10 antibody (BioLegend cat #501420), FITC anti-human FOXP3 antibody (BioLegend cat #320106). Corresponding isotype controls were used for each antibody. All cells were analyzed with BD LSR II flow cytometer using DIVA software version 8.0.
Apc cyanine7 anti human cd138 antibody
Manufactured by BioLegend
The APC/Cyanine7 anti-human CD138 antibody is a fluorochrome-conjugated monoclonal antibody that binds to the CD138 antigen expressed on the surface of cells. CD138, also known as Syndecan-1, is a transmembrane heparan sulfate proteoglycan that plays a role in cell-cell and cell-matrix interactions. The APC/Cyanine7 fluorescent label allows for the detection and analysis of CD138-positive cells using flow cytometry and other fluorescence-based techniques.
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Lab products found in correlation
Apc anti human cd19 antibody, by BioLegend (2 mentions)
Fitc anti human foxp3 antibody, by BioLegend (2 mentions)
Ficoll gradient, by Axis-Shield (2 mentions)
Percp cyanine5.5 anti human cd1d antibody, by BioLegend (2 mentions)
Apc anti human cd25 antibody, by BioLegend (2 mentions)
Pe anti human cd223 lag 3 antibody, by BioLegend (2 mentions)
Pe anti human ebi3 antibody, by BioLegend (2 mentions)
Pe cy7 anti human il 10 antibody, by BioLegend (2 mentions)
Lsr 2 flow cytometer, by BD (2 mentions)
2 protocols using apc cyanine7 anti human cd138 antibody
1
Characterization of Immune Cell Subsets in PBMCs
2
Phenotypic Characterization of PBMCs
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Isolation of peripheral blood mononuclear cells (PBMCs) was performed using a Ficoll gradient (Axis-Shield, Norway). The unstimulated PBMCs were stained with APC anti-human CD19 antibody (BioLegend cat #302212), APC/Cyanine7 anti-human CD138 antibody (BioLegend cat #356528), PerCP/Cyanine5.5 anti-human CD1d antibody (BioLegend cat #350312), APC anti-human CD25 antibody (BioLegend cat #302610), and PE anti-human CD223 (LAG-3) antibody (BioLegend cat #369306). Following surface staining, cells were stained with PE anti-human EBi3 antibody (BioLegend cat #360904), PE/Cy7 anti-human IL-10 antibody (BioLegend cat #501420), and FITC anti-human FOXP3 antibody (BioLegend cat #320106). For each antibody, the corresponding isotype controls were used. All cells were analyzed with BD LSR II flow cytometer using DIVA software version 8.0.
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