Dnasel

Example 4

The mice were randomly divided into groups comprising the same number of animals. The mice were anesthetized with 5% isoflurane (Baxter, Unterschleißheim, Germany) and maintained with 2.5% isoflurane that was delivered through a facemask. Preoperative antisepsis was performed with betaisadona (Nundipharma GmbH, Limburg, Germany). All mice received 0.02 mg/kg buprenorphine (Reckitt Benckiser, Mannheim, Germany) for analgesia subcutaneously.

After median laparotomy the thermic lesions were induced by heat exposure after the viscera (especially the intestine) using a red lamp with a distance of 1 meter for 10 minutes. No further manipulation was performed.

The animals were treated as follows:

• • In the controls, only adhesion induction, but no other intervention was performed.
  • In the DNasel multi group, the abdomen of the mice was washed before closure with 1 ml NaCl 0.9% comprising 10 mg/kg bodyweight DNasel (Pulmozyme, Roche, Grenzach, Germany). Additionally, 100 μl NaCl 0.9% was injected i.p. after 24 and 48 hours.

The abdomen war closed using a 6x0 Ethilon (Ethicon Norderstedt, Germany) running suture.

Example 2

The mice were randomly divided into groups comprising the same number of animals. The mice were anesthetized with 5% isoflurane (Baxter, Unterschleißheim, Germany) and maintained with 2.5% isoflurane that was delivered through a facemask. Preoperative antisepsis was performed with betaisadona (Mundipharma GmbH, Limburg, Germany). All mice received 0.02 mg/kg buprenorphine (Reckitt Benckiser, Mannheim, Germany) for analgesia subcutaneously. Deserositation was induced by rubbing a mini-prep on the small intestinal wall (distal ileum). No further manipulation was performed.

The animals were treated as follows:

• • In the controls, only adhesion induction, but no other intervention was performed.
  • In the DNasel multi group, the abdomen of the mice was washed before closure with 1 ml NaCl 0.9% comprising 10 mg/kg bodyweight DNasel (Pulmozyme, Roche, Grenzach, Germany). Additionally, 100 μl NaCl 0.9% was injected i.p. after 24 and 48 hours.

The abdomen war closed using a 6x0 Ethilon (Ethicon Norderstedt, Germany) running suture.

Example 3

The mice were randomly divided into groups comprising the same number of animals. The mice were anesthetized with 5% isoflurane (Baxter, Unterschleißheim, Germany) and maintained with 2.5% isoflurane that was delivered through a facemask. Preoperative antisepsis was performed with betaisadona (Mundipharma GmbH, Limburg, Germany). All mice received 0.02 mg/kg buprenorphine (Reckitt Benckiser, Mannheim, Germany) for analgesia subcutaneously. Intestinal anastomosis was performed with 8x0 Vicryl (Ethicon Norderstedt, Germany) continuous suture after dissection of the small intestine (distal ileum).

The animals were treated as follows:

• • In the controls, only adhesion induction, but no other intervention was performed.
  • In the DNasel multi group, the abdomen of the mice was washed before closure with 1 ml NaCl 0.9% comprising 10 mg/kg bodyweight DNasel (Pulmozyme, Roche, Grenzach, Germany). Additionally, 100 μl NaCl 0.9% was injected i.p. after 24 and 48 hours.

The abdomen war closed using a 6x0 Ethilon (Ethicon Norderstedt, Germany) running suture.