It has previously been shown that overexpression of sFlt-1 and sEndoglin into normal pregnant rats creates a biochemical profile (hemolysis-increased lactate dehydrogenase; elevated liver enzymes-increased alanine aminotransferase and low platelet count) of HELLP in pregnant rats (11 (link)). In the current study, we infused sFlt-1 and sEndoglin into normal pregnant rats (HELLP rats, n = 16) beginning on gestational day (GD) 12. sFlt-1 and sEndoglin (R&D Systems, Minneapolis, MN) were infused at a rate of 4.7 and 7 μg/kg/day, respectively, for 8 days (days 12–19 of gestation) via mini-osmotic pumps (model 2002; Alzet Scientific, Cupertino, CA) which were surgically inserted into the intraperitoneal cavity of normal pregnant rats. Mini-osmotic pumps containing sFlt-1 (4.7 μg/kg/day) +sEndoglin (7 μg/kg/day) were surgically inserted into the intraperitoneal cavity of virgin rats (n = 4) for a period of 8 days to determine if a similar biochemical profile is seen in non-pregnant rats; these results were compared to virgin control rats (n = 4).
Sendoglin
Manufactured by R&D Systems
Sourced in United States
SEndoglin is a recombinant human Endoglin protein. Endoglin is a transmembrane glycoprotein that is part of the TGF-beta receptor complex. It functions as a co-receptor for TGF-beta superfamily ligands, including TGF-beta1, TGF-beta3, and BMP-2.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using sendoglin
1
Infusion of sFlt-1 and sEndoglin in Pregnant Rats
2
ELISA Validation for Biomarker Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Enzyme-linked immunosorbent assays (ELISAs) were performed using commercial kits. Human EGFL7 kits were purchased from Cusabio Biotech (College Park, Maryland, USA). Human sFLT-1, free PlGF, and sENDOGLIN kits were purchased from R&D Systems (Minneapolis, Minnesota, USA). Assays were performed according to the manufacturer’s instructions in the Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy. Data on spike/recovery, inter- and intra- assay precision were provided by the manufacturer. In details, intra-assay precision was indicated as coefficient of variability (CV) % and calculated as < 8% by testing three samples of known concentration twenty times on one plate. Inter-assay precision was reported as CV% < 10% and it was obtained by testing three samples of known concentration in twenty assays. To confirm these data, we tested five different plasma samples in at least three different assays and obtained comparable results. In each ELISA, all samples and standards have been tested in duplicate; duplicates always gave similar values of optical density (OD).
3
Quantification of Angiogenic Markers
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