F 4500 fluorescence spectrometer system

We used a XYZ3000 distribution platform and CM2000 guillotine cutter to prepare test strips (BioDot, Irvine, CA, USA). A Hitachi F−4500 fluorescence spectrometer system (Hitachi, Tokyo, Japan) was used to record the fluorescence spectra. An FIC−S2011−B14 Dry Fluorescence Immunoassay Analyzer was used to read the test strips (Suzhou Hellman Precision Instruments, Suzhou, China). The immunochromatographic strip results were compared with the data obtained using an Agilent 1100 high-performance liquid chromatography system (Agilent Tech, Santa Clara, CA, USA). Hair was crushed with a ball mill (Beijing Wanfu Intelligent Technology Co., Ltd., Beijing, China).

The size and surface morphology of UCNPs were characterized by transmission electron microscope (TEM, HITACHI, Japan). The F-4500 fluorescence spectrometer system (HITACHI, Japan) adapted with a 980 nm laser device (Changchun Laser Optoelectronics Technology, China) was used to determine the fluorescent spectrum. Immunoreagents were dispensed on nitrocellulose membrane by R5DDA dispense platform (Han'gan, China). Strips were prepared by a cutter (Han'gan, China). The UCNP-based LFIC (UCNP-LFIC) strips were scanned by a strip reader (Suzhou Helmen Precise Instruments, China) with 980 nm near-infrared laser excitation. An ML-902 magnetic stirring apparatus (Pujiang, Shanghai), Allegra 64R super centrifuge (Beckman, America), electric jacket and ultrasonic cleaner were also used in this study.

The role of the Hitachi F-4500 fluorescence spectrometer system (Hitachi, Tokyo, Japan) was used to read and record the fluorescence pattern. An XYZ3000 dispensing platform and a CM2000 guillotine cutter (BioDot, Irvine, CA, United States) was used to prepare and cut test strips. The purpose of the FIC-S2011-B14 fluorescent strip reader (Suzhou, Jiangsu, China) was used to read and record the results of the fluorometric test strip assay. Agilent 1,100 high performance liquid chromatography system (Agilent Tech, Santa Clara, CA, United States) was used to monitor the results of validated fluorescent test strips.