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Nlrc4 antibody

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The NLRC4 antibody is a laboratory reagent used in research applications. It is a specific antibody that binds to the NLRC4 protein, which is a member of the NOD-like receptor (NLR) family and plays a role in the innate immune response. The NLRC4 antibody can be used to detect and study the NLRC4 protein in various experimental systems, but its intended use is not provided.

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Lab products found in correlation

Pro caspase1 antibody, by Cell Signaling Technology (2 mentions) Cleaved caspase 1 antibody, by Cell Signaling Technology (2 mentions) Hspa8 antibody, by Cell Signaling Technology (2 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Cell Signaling Technology (2 mentions)

2 protocols using nlrc4 antibody

1

NLRC4 and Caspase-1 Activation in THP1 Cells

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5×105 transduced THP1 cells were subjected to SDS-PAGE, transferred to nitrocellulose membranes and probed with appropriate antibodies. Antibodies used for protein blots were NLRC4 antibody (1:500 dilution; Millipore, 06-1125), pro-caspase1 antibody (1:1000 dilution; Cell Signaling Technology, 2225), cleaved caspase-1 antibody (1:500 dilution; Cell Signaling Technologies, 4199), HSPA8 antibody (1:2000 dilution; Cell Signaling Technologies, 8444). Horseradish peroxidase (HRP)-conjugated secondary antibodies (Cell Signaling Technologies, 7074) and SuperSignal West Pico chemiluminescent substrate (Pierce) were used to detect primary antibodies. Equal protein loading was assessed by immunoblotting for HSPA8.
Canna S.W., de Jesus A.A., Gouni S., Brooks S.R., Marrero B., Liu Y., DiMattia M.A., Zaal K.J., Montealegre Sanchez G.A., Kim H., Chapelle D., Plass N., Huang Y., Villarino A.V., Biancotto A., Fleisher T.A., Duncan J.A., O’Shea J.J., Benseler S., Grom A., Deng Z., Laxer R.M, & Goldbach-Mansky R. (2014). An activating NLRC4 inflammasome mutation causes autoinflammation with recurrent macrophage activation syndrome. Nature genetics, 46(10), 1140-1146.
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2

NLRC4 and Caspase-1 Activation in THP1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
5×105 transduced THP1 cells were subjected to SDS-PAGE, transferred to nitrocellulose membranes and probed with appropriate antibodies. Antibodies used for protein blots were NLRC4 antibody (1:500 dilution; Millipore, 06-1125), pro-caspase1 antibody (1:1000 dilution; Cell Signaling Technology, 2225), cleaved caspase-1 antibody (1:500 dilution; Cell Signaling Technologies, 4199), HSPA8 antibody (1:2000 dilution; Cell Signaling Technologies, 8444). Horseradish peroxidase (HRP)-conjugated secondary antibodies (Cell Signaling Technologies, 7074) and SuperSignal West Pico chemiluminescent substrate (Pierce) were used to detect primary antibodies. Equal protein loading was assessed by immunoblotting for HSPA8.
Canna S.W., de Jesus A.A., Gouni S., Brooks S.R., Marrero B., Liu Y., DiMattia M.A., Zaal K.J., Montealegre Sanchez G.A., Kim H., Chapelle D., Plass N., Huang Y., Villarino A.V., Biancotto A., Fleisher T.A., Duncan J.A., O’Shea J.J., Benseler S., Grom A., Deng Z., Laxer R.M, & Goldbach-Mansky R. (2014). An activating NLRC4 inflammasome mutation causes autoinflammation with recurrent macrophage activation syndrome. Nature genetics, 46(10), 1140-1146.
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