Texture of the recovered samples was observed using a Zeiss LEO 1530 Gemini field-emission-type scanning electron microscope with an Oxford X-MaxN energy-dispersive X-ray spectrometer. The phases present in the recovered samples were confirmed using an in-house micro-focused X-ray diffractometer (Brucker AXS Discover 8).
Leo 1530 gemini
Manufactured by Zeiss
Sourced in Germany
The LEO 1530 Gemini is a high-performance scanning electron microscope (SEM) designed for advanced materials research and analysis. It features a Gemini electron column, which delivers high-resolution imaging and exceptional analytical capabilities. The LEO 1530 Gemini is capable of producing detailed images and performing in-depth elemental analysis of a wide range of samples.
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Lab products found in correlation
Glutaraldehyde, by Merck Group (2 mentions)
Dxr raman microscope, by Thermo Fisher Scientific (2 mentions)
Cpd300, by Leica camera (2 mentions)
Noran x ray detector, by Thermo Fisher Scientific (2 mentions)
Noran system six, by Thermo Fisher Scientific (2 mentions)
Atlas scanning generator, by Zeiss (2 mentions)
Axioscope a1, by Canon (1 mentions)
Nanoscope dimension 5, by Veeco (1 mentions)
Otespa r3 cantilevers, by Bruker (1 mentions)
Mastersizer 3000, by Malvern Panalytical (1 mentions)
Tecnai g2, by Thermo Fisher Scientific (1 mentions)
T64000, by Horiba (1 mentions)
D max 2550 pc, by Rigaku (1 mentions)
Schottky field emitter, by Zeiss (1 mentions)
Multimode 8 spm, by Bruker (1 mentions)
Inlens se detector, by Zeiss (1 mentions)
Smart sem v05, by Zeiss (1 mentions)
Dmc 4500, by Leica camera (1 mentions)
Hkl channel 5, by Oxford Instruments (1 mentions)
D8 advance x ray, by Bruker (1 mentions)
90 protocols using leo 1530 gemini
1
Characterization of Recovered Samples
2
Scallop Valve Surface Characterization
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The exterior surfaces of Antarctic scallop valves were analyzed using scanning electron microscopy (SEM; Zeiss, LEO 1530 Gemini). Atomic force microscopy (AFM) was used to provide nanometer-resolution mapping (300 kHz, tapping mode) of surface geometries and surface roughness (4-µm2 scan area) on the Antarctic scallop. The surface elemental composition and its variation across the exterior valve surface were determined using energy-dispersive X-ray spectroscopy (EDS) for Ca, C, O, F, Na, Mg, Al, Si, and Ca. This was performed on the growth rings, micro-/nano-ridges, and micro-valleys.
3
Probing Ferroelastic Domains in BFO
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The distribution of ferroelastic domains was verified by electron backscatter diffraction (EBSD) measurements that were performed on a scanning electron microscope (Zeiss LEO 1530 GEMINI) operating at an acceleration voltage of 20 kV. The Kikuchi patterns were recorded with a Nordlys II detector (Oxford Instruments) at the step size of 0.2 µm, and subsequently indexed by the HKL Channel 5 software. For indexing, the crystal structure suggested by Moreau et al.6 (link) was used. The EBSD measurements confirmed the almost single-crystalline nature of the BFO crystals and their orientation (001)pc parallel with the sample surface. Because of the pseudo-cubic symmetry of BFO and the centrosymmetry of the EBSD patterns, EBSD cannot distinguish easily between the orientation variants, which are mutually rotated about approximately 90°, 180° or 270° around the axis perpendicular to the sample surface32 (link). Still in this particular case, the EBSD measurement was able to trace the local orientation of the polarisation axis through the elastic distortion of the crystal structure that is caused by the polarisation/ferroelastic effect. Thus, the smallest mean angular deviation (MAD) between the measured and simulated Kikuchi patterns of BFO was utilized as a decision criterion for the respective orientation variant and for the corresponding orientation of the polarisation axis.
4
Microscopic Analysis of Cone Fragment Remains
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Samples of the dark remains of the cone fragment were removed using a sharpened needle and prepared for both scanning electron and light microscopy. For scanning electron microscopy (SEM), small pieces of the tissue were transferred to a carbon-covered SEM mount using a wet hair from a superfine brush. The stubs were sputtered with platinum–palladium (2 × 120 s at 20 mA, 10 nm coat thickness) using an automatic sputter coater (Canemco Inc.) and examined under a field emission scanning electron microscope (Carl Zeiss LEO 1530 Gemini).
For light microscopy (LM), the samples picked with needles were treated with Schultze solution (concentrated nitric acid saturated with a few potassium chlorate crystals added) and washed in distilled water, then treated with 4% ammonia and washed until neutral. The extracted material was then mounted on microscopy slides in glycerine jelly, and covered with a coverslip. These pollen preparations were observed with a transmitted light microscope (Carl Zeiss Axioscope A1) equipped with a Canon 450D digital camera, and are deposited with the source specimen (MB.Pb.1997/1246).
For light microscopy (LM), the samples picked with needles were treated with Schultze solution (concentrated nitric acid saturated with a few potassium chlorate crystals added) and washed in distilled water, then treated with 4% ammonia and washed until neutral. The extracted material was then mounted on microscopy slides in glycerine jelly, and covered with a coverslip. These pollen preparations were observed with a transmitted light microscope (Carl Zeiss Axioscope A1) equipped with a Canon 450D digital camera, and are deposited with the source specimen (MB.Pb.1997/1246).
5
Electrospun Scaffold Fiber Characterization
6
Scanning Electron Microscopy of Olfactory Structures in C. marinus
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For investigation of peripheral olfactory structures we used female, male and larval C. marinus preserved in 70% ethanol. Tissue was dehydrated in an ascending ethanol series (80%, 90% 96%, 3 × 100% ethanol, 10 min each), critical point dried (BAL-TEC CPD 030, Bal-Tec Union Ltd., Liechtenstein), mounted on aluminium stubs with adhesive tape and sputter coated with gold on a BAL-TEC SCD005 (Bal-Tec, Liechtenstein). Specimens were examined in a LEO 1530 Gemini scanning electron microscope (Zeiss, Germany) set at 10 kV and 11 to 16 mm working distance.
7
SEM analysis of metal oxide nanoparticles and Campylobacter
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Scanning electron microscopy (SEM) was applied to investigate the interaction between metal oxide nanoparticles and C. jejuni cells. C. jejuni strain F38011 (OD540 ∼0.3) was treated with 1 mM ajoene, 16 mM Al2O3 nanoparticles, 16 mM TiO2 nanoparticles, a combination of 0.06 mM ajoene and 4 mM Al2O3 nanoparticles, and a combination of 0.06 mM ajoene and 4 mM TiO2 nanoparticles, respectively, for 1 h at 37°C in microaerobic conditions. The untreated and treated bacterial samples were washed three times with sterilized water and collected by centrifugation at 8,000 × g for 10 min at 4°C. Bacteria pellet was individually fixed with 2.5% (w/v) glutaraldehyde at 4°C for overnight. The samples were then rinsed twice with 0.1 M phosphate buffer, dehydrated in a series of concentrations of ethanol (25, 50, 70, 80, 90, 10 min for each concentration) and post-fixed using 1% (w/v) osmium tetroxide for 1 h. The reaction mixture was freeze-dried in a lyophilizer (Christ, Osterode, Germany). The freeze-dried samples were coated with a layer of gold and then examined by a scanning electron microscope with an accelerating voltage of 5 kV (Leo 1530 Gemini, Zeiss, Jena, Germany).
8
Multiscale Characterization of Metallic Nanostructures
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Scanning electron microscopy (SEM) images are obtained with an LEO 1530 Gemini field
emission scanning electron microscope (Carl Zeiss AG, Oberkochen, Germany). The In Lense
detector is used for imaging all samples, and the gun’s acceleration voltage is set
between 2 and 3 kV.
Atomic force microscopy (AFM) images are recorded using a Nanoscope Dimension V (Veeco,
USA) instrument with a Dimension 3100 controller. The instrument is operated in the
tapping mode with OTESPA-R3 cantilevers (Bruker). The resulting images are evaluated with
NanoScope Analysis software. Moreover, a slope analysis was performed on each sample to
verify the sharpness of the fabricated Au discs. Details of the slope analysis are
presented in theSupporting Information .
Additionally, 3D reconstructed optical images taken with a laser scanning microscope
(Olympus, LEXT) are used to show the uniformity of the samples regarding the periodicity
and geometry of the discs. This laser scanning microscope allows a fast qualitative
evaluation of the fabricated samples. A 100× objective was used with N.A. 0.95 and a
z-scanning pitch of 120 nm. Additionally, a 1.3× digital zoom was used to obtain a
better view of the Au discs imaged.
emission scanning electron microscope (Carl Zeiss AG, Oberkochen, Germany). The In Lense
detector is used for imaging all samples, and the gun’s acceleration voltage is set
between 2 and 3 kV.
Atomic force microscopy (AFM) images are recorded using a Nanoscope Dimension V (Veeco,
USA) instrument with a Dimension 3100 controller. The instrument is operated in the
tapping mode with OTESPA-R3 cantilevers (Bruker). The resulting images are evaluated with
NanoScope Analysis software. Moreover, a slope analysis was performed on each sample to
verify the sharpness of the fabricated Au discs. Details of the slope analysis are
presented in the
Additionally, 3D reconstructed optical images taken with a laser scanning microscope
(Olympus, LEXT) are used to show the uniformity of the samples regarding the periodicity
and geometry of the discs. This laser scanning microscope allows a fast qualitative
evaluation of the fabricated samples. A 100× objective was used with N.A. 0.95 and a
z-scanning pitch of 120 nm. Additionally, a 1.3× digital zoom was used to obtain a
better view of the Au discs imaged.
9
Scanning Electron Microscopy of PUF and CNF-PUF
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A scanning electron microscope (Zeiss LEO 1530 Gemini, Oberkochen, Germany) with an acceleration voltage of 5 kV was used to evaluate the morphology of PUF and CNF-PUF samples. Thin slices of 10 mm × 10 mm × 3 mm were cut from the foams using a stainless steel blade and then mounted onto carbon tapes on the aluminum stubs. Samples were then sputter coated with gold (Denton High Vacuum Coating System, Moorestown, NJ, USA) for 1 min under vacuum. The working distance was set at 5 mm.
10
Particle Characterization of Powder Samples
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Particle size, morphology and chemical composition of the powdered samples were investigated using a LEO 1530 Gemini (Carl Zeiss AG) in combination with EDX at an acceleration voltage of 10 kV. Before measurement, the particulate samples were suspended in H2O, applied onto a silicon specimen holder, dried and sputtered with platinum to increase conductivity.
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