C18 1

Manufactured by Merck Group

Sourced in Germany, United States

C18:1 is a type of laboratory equipment used for various analytical and preparative applications. It is a long-chain fatty acid with an 18-carbon backbone and a single double bond. The core function of C18:1 is to serve as a stationary phase in chromatographic techniques, such as high-performance liquid chromatography (HPLC) and gas chromatography (GC), for the separation and purification of a wide range of organic compounds.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using c18 1

Optimized PHA Production Using Carboxylic Acids

Check if the same lab product or an alternative is used in the 5 most similar protocols

The minimal medium for PHA production contained, except otherwise stated (g L⁻¹): 3.5 NaNH₄HPO₄.4H₂O (Sigma Aldrich, Germany); 5.7 K₂HPO₄, 3.7 KH₂PO₄, and 10 mM carboxylic acid puriss i.e. C_8:0, C_12:0, C_16:0 and C_18:1 (Sigma Aldrich, Germany) at pH 7.0 (± 0.1). The pure carboxylic acid was used a sole carbon and energy source during the fermentation. Sterile 1.0% (v/v) Mg₂SO₄.7H₂O solution and 0.1% (v/v) trace elements (MT) solution containing (g L⁻¹): CaCl₂.2H₂O 1.47, CoCl₂.6H₂O 2.38, CuCl₂.2H₂O 0.17, FeSO₄.7H₂O 2.78, MnCl₂.4H₂O 1.98 and ZnSO₄.7H₂O 0.29 dissolved in 1 M HCl were aseptically and separately added prior to inoculum seeding. 3% (v/v) of harvested biomass suspended in phosphate buffer was seeded into this medium aseptically and then incubated at 30 °C, 250 rpm for a total period of 48 h except otherwise stated.

Gumel A.M., Annuar M.S, & Heidelberg T. (2014). Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet001. Brazilian Journal of Microbiology, 45(2), 427-438.

+ Open protocol

+ Expand

Fatty Acid Determination Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chloroform, ethyl alcohol (96% w/w), methanol, n-hexane, formic acid (99% w/w) hydrochloric acid (37% w/w) and anhydrous sodium sulphate were of analytical grade and were purchased from Carlo Erba (Milan, Italy). Boron trifluoride (approximately 10% w/w in methanol for gas chromatography (GC) derivatization) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Fatty acid standards (C16:0, C16:1, C17:0, C18:0, C18:1, C18:2, C18:3) were also purchased from Sigma-Aldrich.

Narducci V., Finotti E., Galli V, & Carcea M. (2019). Lipids and Fatty Acids in Italian Durum Wheat (Triticum durum Desf.) Cultivars. Foods, 8(6), 223.

+ Open protocol

+ Expand

Lipid Derivatization and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

An AccQ•Tag Ultra derivatization kit (borate buffer and reagent) and AccQ•Tag Ultra eluents A and B were obtained from Waters (Milford, MA, USA). Ammonium formate, formic acid, and potassium hydroxide were acquired from Sigma-Aldrich (St. Louis, MO, USA). Hydrochloric acid solution and sodium hydroxide were purchased from Daejung (Seoul, Korea). HPLC-grade methanol, ethyl acetate, isopropyl alcohol, and chloroform were purchased from Burdick & Jackson (Muskegon, MI, USA). CHOL and the FFAs (C12, C14, C16, C16:1, C18, C18:1, C18:2, C20, C22, C22:1, C24, C24:1, C26, and C28) were obtained from Sigma-Aldrich. Deuterated FFAs C16-D₃, C20-D₃, and C18:1-D₂ were purchased from Cambridge Isotope Laboratories (Andover, MA, USA). Synthetic CER-NP, CER-NDS, and CER-NS were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Synthetic CER-AP was kindly provided by Evonik (Essen, Germany). 2,2′-dithiodipyridin, 2-picolylamine, and triphenylphosphine were purchased from Sigma-Aldrich. All other reagents used were of the highest grade available. The water used was ultra-pure deionized water (18.2 MΩ•cm) produced using a Milli-Q Gradient system (Millipore, Bedford, MA, USA).

Kim M., Jeong H., Lee B., Cho Y., Yoon W.K., Cho A., Kwon G., Nam K.T., Ha H, & Lim K.M. (2019). Enrichment of Short-Chain Ceramides and Free Fatty Acids in the Skin Epidermis, Liver, and Kidneys of db/db Mice, a Type 2 Diabetes Mellitus Model. Biomolecules & Therapeutics, 27(5), 457-465.

+ Open protocol

+ Expand

Analytical Reagent Standards Procurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemical reagents were of recognized analytical quality. Water was either distilled or of equivalent purity. The standards of C17:0 ME and C18:1, phenolphthalein, potassium hydroxide, silica gel, 60-200 μm mesh, and Sudan I (1-phenylazo-2-naphthol) were purchased from Sigma (St. Louis, MO, USA).
EtOH, ethyl ether, n-hexane, n-heptane, MeOH and PrOH were from Romil Ltd.
(Waterbeach, Cambridge, GB).

Gómez-Coca R.B., Fernandes G.D., Pérez-Camino M.d.C, & Moreda W. (2016). Fatty acid ethyl esters (FAEE) in extra virgin olive oil: A case study of a quality parameter. Lebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire, 66.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!