Azd6244

Manufactured by Chemietek

Sourced in United States

AZD6244 is a laboratory instrument used for chemical analysis and research. It is designed to perform specific tasks, but a detailed description cannot be provided while maintaining an unbiased and factual approach. The core function of this product is not available for reporting at this time.

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Lab products found in correlation

5 protocols using azd6244

Cell Culture and Compound Treatment

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A375, 293T, SKMEL-19, and WM266.4 cells were grown in DMEM with 10% FBS. A375 and 293T cells were acquired from the ATCC. WM266.4 cells were acquired through the Cancer Cell Line Encyclopedia (20 ). SKMEL-19 cells were a gift from N. Rosen (Memorial Sloan Kettering Cancer Center, New York, NY). GSK1120212, GSK2118436, VX-11e, and AZD6244 were obtained from Chemietek. PLX4720 was obtained from Selleck. SCH772984 was synthesized by J & W PharmLab. Lentiviral production and infection were performed as previously described (19 (link)).

Goetz E.M., Ghandi M., Treacy D.J., Wagle N, & Garraway L.A. (2014). ERK Mutations Confer Resistance to Mitogen-Activated Protein Kinase Pathway Inhibitors. Cancer research, 74(23), 7079-7089.

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Evaluating Efficacy of Targeted Therapies

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NVP-BEZ235 (BEZ235), NVP-BKM120 (Buparlisib, BKM120), GSK1120212 (Trametinib, JTP-74057), PD0325901, and AZD6244 were purchased from Chemie Tek (Indianapolis, IN). Temozolomide, doxorubicin and paclitaxel were purchased from Sigma. For in vivo studies, BEZ235 and BKM120 were resuspended in NMP (N-methyl-2-pyrrolidone):PEG300 1:9 v:v, and GSK1120212 and PD0325901 were resuspended in 0.5% hydroxypropyl-methylcellulose (Sigma):0.2% Tween 80 (Sigma) (Gilmartin et al., 2011 (link); Kinross et al., 2011 (link)). BKM120, GSK1120212 and PD0325901 were given once daily by oral gavage at 10 ml/kg. Mice were treated with BEZ235 on a 5 days on, 2 days off schedule (Liu et al., 2009 (link)). Vehicle- and drug-treated mice were closely monitored on a daily basis for clinical signs as described above.

El Meskini R., Iacovelli A.J., Kulaga A., Gumprecht M., Martin P.L., Baran M., Householder D.B., Van Dyke T, & Weaver Ohler Z. (2014). A preclinical orthotopic model for glioblastoma recapitulates key features of human tumors and demonstrates sensitivity to a combination of MEK and PI3K pathway inhibitors. Disease Models & Mechanisms, 8(1), 45-56.

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Evaluation of Small Molecule Inhibitors

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17-AAG (tanespimycin), NVP-AUY922, AZD6244, and NVP-BEZ235 were purchased from ChemieTek (Indianapolis, IN) and ES936 and gemcitabine from Tocris Bioscience (Bristol, United Kingdom), and each one of them is dissolved in DMSO or water. Propidium iodide, crystal violet, iodonitrotetrazolium violet, 4-hydroxycoumarin (dicumarol), 2,6-dichlorophenol-indophenol (DCPIP), and oxaliplatin were purchased from Sigma-Aldrich (St Louis, MO).

Mayor-López L., Tristante E., Carballo-Santana M., Carrasco-García E., Grasso S., García-Morales P., Saceda M., Luján J., García-Solano J., Carballo F., de Torre C, & Martínez-Lacaci I. (2014). Comparative Study of 17-AAG and NVP-AUY922 in Pancreatic and Colorectal Cancer Cells: Are There Common Determinants of Sensitivity?. Translational Oncology, 7(5), 590-604.

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Evaluating Compounds for Cancer Therapeutics

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LBH589, erlotinib, BIBW2992, WZ4002, BEZ235, and AZD6244 were purchased from Chemie Tek (Indianapolis, IN). PD173074, LY364947, salinomycin, and IWP2 were purchased from Sigma-Aldrich (St. Louis, MO). CNTO328 was provided by Centocor, Inc. (Horsham, PA). CL-387,785 was purchased from AXXORA (San Diego, CA). Stock solutions of these reagents in 100% DMSO were diluted directly into the media to indicated concentrations. Human TGF-β1 was purchased from R&D Systems (Minneapolis, MN).

Yoshida T., Song L., Bai Y., Kinose F., Li J., Ohaegbulam K.C., Muñoz-Antonia T., Qu X., Eschrich S., Uramoto H., Tanaka F., Nasarre P., Gemmill R.M., Roche J., Drabkin H.A, & Haura E.B. (2016). ZEB1 Mediates Acquired Resistance to the Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitors in Non-Small Cell Lung Cancer. PLoS ONE, 11(1), e0147344.

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Small Molecule Screening for Cancer

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Gefitinib, erlotinib, sorafenib, 17-AAG, NVP-BEZ235 and AZD-6244 were obtained from ChemieTek (Indianapolis, IN, USA). Rapamycin, tricostatin (TSA), propidium iodide and 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RNase A was obtained from Serva (Heidelberg, Germany).

Grasso S., Martínez-Lacaci I., Barberá V.M., Castillejo A., Soto J.L., Gallego-Plazas J., López-Riquelme N., García-Morales P., Mata-Balaguer T., Ferragut J.A, & Saceda M. (2015). HGUE-C-1 is an atypical and novel colon carcinoma cell line. BMC Cancer, 15, 240.

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