Dried sample extracts were used for GC-MS analysis. Untargeted metabolite profiling was performed by GC-MS using an Agilent 7890A-5975C GC-MSD after derivatization of metabolites with methoxyamine hydrochloride (20 mg/ml in pyridine, both Sigma) and N,O-bis-(trimethylsilyl)trifluoroacetamide (containing 1% trimethylchlorosilane)57 (link). GC separation was achieved using an Agilent DB-5 MS column (30 m × 0.25 mm × 0.5 μm). The GC oven temperature program was: 70 °C, 2 min hold; ramp 12.5 °C/min to 295 °C, 0 min hold; ramp 25 °C/min to 320 °C, 3 min hold. Other GC parameters were: injection volume 1 μl; inlet temperature 270 °C; Helium was used as a carrier gas at a flow rate of 0.9 ml/min; transfer line temperature 280 °C. Electron impact ionization was used for mass spectrometry detection with scan range m/z 50–565.
7890a gc 5975c msd
Manufactured by Agilent Technologies
Sourced in United States
The 7890A GC-5975C MSD is a gas chromatograph-mass spectrometer (GC-MS) system designed for analytical applications. It is capable of separating and identifying chemical compounds in complex samples. The 7890A GC provides high-performance gas chromatography, while the 5975C MSD offers sensitive mass spectrometric detection. This system is suitable for a wide range of analytical tasks, including environmental, food, and pharmaceutical analyses.
Automatically generated - may contain errors
9 protocols using 7890a gc 5975c msd
1
GC-MS Metabolite Profiling of Botanical Extracts
2
Standardized E. coli and Streptomyces Protocols
3
Glucose and Lactate Metabolism Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glucose and lactate were analyzed in whole blood by use of an automated biochemical analyzer (YSI 2300 Stat Plus; YSI, Yellow Springs, OH). Plasma insulin concentrations were determined with a commercially available enzyme-linked immunosorbent assay (ELISA) (ALPCO Diagnostics, Salem, NH) and expressed as area under the curve (AUC) during the cycling challenge. Plasma I-FABP concentrations were assessed with an ELISA according to the manufacturer’s instructions (Hycult Biotechnology, Uden, The Netherlands) and was expressed as fold change from baseline. Plasma [U-13C6]glucose enrichments were determined by gas chromatography-mass spectrometry (GC-MS) analysis (7890A GC/5975C MSD, Agilent). Briefly, plasma samples were deproteinized and converted into their tert-butyldimethylsilyl derivatives, and enrichments were determined using electron ionization by ion monitoring at m/z of 319 (m+0), 321 (m+2), and 323 (m+4). Plasma glucose enrichments for each labeled ion were expressed relative to 319 (m+0, tracee), and enrichment was expressed as tracer-to-tracee-ratio (TTR). All blood metabolites were analyzed blindly.
4
Comprehensive 2D-LC/2D-GC-MS System
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the instrumentation for the 2D-LC/2D-GC system was described in the previous study [25 (link)]. The 2D-LC system was composed of three LC columns: C18, 250 mm × 4.6 mm, 5 μm (Phenomenex, Torrance, CA, USA); Cosmosil 5-pentabromobenzyloxypropyl, 150 mm × 4.6 mm, 5 μm (Nacalai Tesque, Kyoto, Japan); and Hypercarb porous graphitic carbon (PGC), 10 mm × 4.6 mm, 3 μm (Thermo Fisher Scientific, Waltham, MA, USA). The 2D-GC system consisted of a 50% phenyl methylpolysiloxane column [low thermal mass (LTM) column module DB-17 ms], 30 m × 0.25 mm, 0.25-μm phase (Agilent Technologies, Folsom, CA, USA), and an LC50 column, 5 m × 0.25 mm, 0.10-μm phase (J&K Scientific, Milton, Canada). In the present study, the first dimension of the 2D-GC system was replaced with a shorter and thinner column (15 m × 0.25 mm, 0.15 μm; LTM DB-17 ms, Agilent Technologies, Folsom, CA, USA). Also, the detection method was modified by our changing the detectors from FIDs to MSDs. This was done by attachment of an additional MSD, a Finnigan TSQ 7000 triple-quadrupole mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), onto the 7890A GC/5975C MSD (Agilent Technologies, Palo Alto, CA, USA) as shown in Fig. S1 .
5
Quantifying PAHs Compounds by GC-MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In order to avoid potential contamination during the experiment, all containers were rinsed with ultrapure water. The Agilent 7890A-GC/5975C-MSD was used for GC–MS analysis. The chromatographic column was HP-5 fused silica capillary column (30 m × 0.25 mm × 0.25 µm), the temperature of the sample inlet is 300 °C, the initial temperature of the column is 50 °C, hold for 5 min, raise the temperature to 220 °C at the rate of 4 °C/min, and raise the temperature to 320 °C at the rate of 2 °C/min, hold for 25 min; The carrier gas is helium, the flow rate is 1 cm3/min, and the scanning mode is full scanning and selective ion scanning. PAHs compounds were identified according to retention time of chromatogram and GC–MS mass spectrometry database; Calculate the PAHs content according to (d10-Phenanthrene) standard sample.
6
Volatile Compound Identification in Mixed Dough
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Agilent 7890A GC‐5975C MSD was used to identify the volatile compounds of the mixed dough. The oven temperature program was set at 35○C for 3 min and then to 280○C at 5○C/min, with a cycle time of 52 min. The temperatures of the injector and ion source were 250○C and 230○C, respectively. Electron ionization mass spectra were recorded at 70 eV. The mass spectrometer was operated in full scan mode, with an m/z range from 40 to 400 amu and a scan time of 0.25 s. Helium was used as a carrier gas at a flow rate of 1 ml/min. The HP‐5MS column had the following dimensions: length: 30 m; internal diameter: 0.25 mm; and film thickness: 0.25 μm. Retention indices (RI) were calculated for each compound using homologous series of C4–C20 n‐alkanes (Dong et al., 2015 ).
7
GC-MS Analysis of J. rubens Extracts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GC-MS (Gas Chromatography-Mass Spectrometry) analysis of J. rubens extracts were performed using a 7890A GC-5975C MSD (Agilent) instrument and HP-5MS column (30 m × 250 µm × 0.25 µm). In the analysis, helium with a flow rate of 1 mL/min was used as the carrier gas, and the furnace temperature was started at 50 °C, kept at this temperature for 3 min, and increased to 300 °C with 10 °C/min increments per minute and kept at this temperature for 6 min. The injection volume for each sample is 1 μL, and the ionization voltage is 70 eV. Separated components were evaluated by comparing them with NIST 2008 (National Institute of Standards and Technology) and National Standards Institute data [41] (link).
8
Serum Fatty Acid Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At 8:00 a.m., 5 mL of fasting venous blood samples were collected with a vacuum serum tube without anticoagulant at the baseline and after 4-week intervention. After standing at room temperature for 1–2 h, blood samples were centrifuged at 3000 rpm for 15 min at 4 °C. The aliquots of the serum were stored at −80 °C for future analysis.
Approximately 200 μL of serum sample were mixed with 400 μL of methanol and 1 mL of n-hexane. The mixture was vortexed thoroughly for 1 min and sonicated for 5 min at 4 °C and then centrifuged at 8000 rpm for 5 min. Then, 750 μL of supernatant were harvested for drying under nitrogen flow. After adding 2 mL of concentrated sulfuric acid/methanol solution with a volume ratio of 5% and 25 μL of butylated hydroxytoluene (BHT)/methanol solution with a mass ratio of 0.2%, the mixture was vortexed for 1 min and heated at 90 °C water bath for 60 min. After cooling to room temperature, the mixture with adding 1 mL of n-hexane and 2 mL of saturated sodium chloride was vortexed thoroughly for 1 min and centrifuged at 3500 rpm for 5 min at 4 °C. Then, 500 μL of supernatant were dried under nitrogen stream after transferred into another tube, and dissolved in 100 μL of n-hexane. Finally, 70 μL of supernatant were used for the analysis of FA profile by gas chromatography coupled with mass selective detector (GC-MSD) (7890A GC-5975C MSD, Agilent, USA).
Approximately 200 μL of serum sample were mixed with 400 μL of methanol and 1 mL of n-hexane. The mixture was vortexed thoroughly for 1 min and sonicated for 5 min at 4 °C and then centrifuged at 8000 rpm for 5 min. Then, 750 μL of supernatant were harvested for drying under nitrogen flow. After adding 2 mL of concentrated sulfuric acid/methanol solution with a volume ratio of 5% and 25 μL of butylated hydroxytoluene (BHT)/methanol solution with a mass ratio of 0.2%, the mixture was vortexed for 1 min and heated at 90 °C water bath for 60 min. After cooling to room temperature, the mixture with adding 1 mL of n-hexane and 2 mL of saturated sodium chloride was vortexed thoroughly for 1 min and centrifuged at 3500 rpm for 5 min at 4 °C. Then, 500 μL of supernatant were dried under nitrogen stream after transferred into another tube, and dissolved in 100 μL of n-hexane. Finally, 70 μL of supernatant were used for the analysis of FA profile by gas chromatography coupled with mass selective detector (GC-MSD) (7890A GC-5975C MSD, Agilent, USA).
9
Monitoring Oil Biodegradation via GC-MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oil biodegradation was monitored through the analysis of residual petroleum hydrocarbons (alkanes and aromatics) in sacrificed microcosms. The analysis was performed by GC-MS (Agilent Technologies 7890A GC-5975C MSD) using an internal standard method described elsewhere (Campo et al., 2013) . Alkanes included normal and branched aliphatics ranging in carbon number from 10 to 35, plus pristane, phytane, and hopane. Aromatics included 2-, 3-, and 4-ring PAH compounds and their alkylated homologs (i.e. C0-4-naphthalenes, C0-3fluorenes, C0-3-dibenzothiophenes, C0-4-phenanthrenes, anthracene, fluoranthene, C0-3-
naphthobenzothiophenes, C0-2-pyrenes, C0-3-chrysenes). Analyte concentrations were normalized to that of hopane, which is assumed to be non-biodegradable throughout the 42-day experiments, to eliminate initial differences in the oil concentration which might have occured through oil losses during its loading in the microcosms (analysis at time t=0) and later during the microcosms sampling and extraction (Campo et al., 2013; Venosa et al., 1996) .
naphthobenzothiophenes, C0-2-pyrenes, C0-3-chrysenes). Analyte concentrations were normalized to that of hopane, which is assumed to be non-biodegradable throughout the 42-day experiments, to eliminate initial differences in the oil concentration which might have occured through oil losses during its loading in the microcosms (analysis at time t=0) and later during the microcosms sampling and extraction (Campo et al., 2013; Venosa et al., 1996) .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!