Hybe solution

Manufactured by Merck Group

Hybe solution is a specialized laboratory reagent used in molecular biology and genetics research. It is designed to facilitate the hybridization process, which is a critical step in various DNA and RNA-based analysis techniques. The core function of Hybe solution is to provide optimal conditions for the formation of stable double-stranded nucleic acid complexes during hybridization experiments.

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Lab products found in correlation

Ap conjugated anti dig antibody, by Roche (3 mentions) Aqua poly mount, by Polysciences (3 mentions) Nbt bcip, by Roche (2 mentions) Eclipse e200, by Nikon (1 mentions)

3 protocols using hybe solution

In Situ Hybridization for C1qa Expression

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For in situ hybridization, an RNA probe for C1qa (GE Dharmacon Clone ID 3592169) was synthesized, labeled with digoxigenin (Dig) and hydrolyzed by standard procedures. Frozen sections were postfixed (4%PFA for 5 min), rinsed twice with 1× PBS and acetylated with 0.25% acetic anhydride for 10 min in 0.1 M triethanolamine (TEA). Sections were then washed in PBS and incubated overnight at 65°C in hybridization solution [50% formamide, 1× Hybe solution (Sigma-Aldrich), 1 mg/ml yeast RNA] containing 1 g/ml Dig-labeled riboprobe. After hybridization, sections were washed by immersion in 0.2× saline-sodium citrate buffer at 72°C for 1 h. Dig-labeled probes were detected with an AP-conjugated anti-Dig antibody (Roche) followed by nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) reaction (Roche). After in situ hybridization, sections were incubated with DAPI for nuclei staining and mounted in Aqua PolyMount (Polysciences) as described previously (Howell et al., 2011 (link)).

Graham L.C., Kocalis H.E., Soto I, & Howell G.R. (2020). Deficiency of Complement Component C1Q Prevents Cerebrovascular Damage and White Matter Loss in a Mouse Model of Chronic Obesity. eNeuro, 7(3), ENEURO.0057-20.2020.

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In Situ Hybridization for Mouse Plp Gene

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For in situ hybridization, a RNA probe for mouse Plp (GE Dharmacon Clone ID: 5364736) was synthesized, labeled with digoxigenin (Dig) and hydrolized. Frozen sections were post fixed (4%PFA for 5min), rinsed twice with 1X PBS and acetylated with 0.25% acetic anhydride for 10min in 0.1M triethanolamine (TEA). Sections were then washed in PBS and incubated overnight at 65°C in hybridization solution [50% formamide, 1X Hybe solution (Sigma-Aldrich), 1mg/ml yeast RNA] containing 1g/ml Dig-labeled riboprobe. After hybridization, sections were washed by immersion in 0.2X saline-Sodium citrate buffer at 72 °C for 1hr. Dig-labeled probes were detected with an AP-conjugated anti-Dig antibody (Roche) followed by NBT/BCIP (nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate) reaction (Roche). After in situ hybridization, sections were incubated with DAPI for nuclei staining and mounted in Aqua-Poly/Mount (Polysciences) as described previously (Howell et al., 2011 (link)). Images taken of Plp in situ hybridization were obtained using a Nikon Eclipse E200 microscope using SPOT Basic 5.2 imaging software.

Graham L., Grabowska W., Chun Y., Risacher S., Philip V., Saykin A., Sukoff Rizzo S, & Howell G. (2019). Exercise prevents obesity-induced cognitive decline and white matter damage in mice. Neurobiology of aging, 80, 154-172.

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In Situ Hybridization for C1qa mRNA Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

For in situ hybridization, an RNA probe for C1qa (GE Dharmacon Clone ID 3592169) was synthesized, labeled with digoxigenin (Dig) and hydrolyzed by standard procedures. Frozen sections were post fixed (4%PFA for 5min), rinsed twice with 1X PBS and acetylated with 0.25% acetic anhydride for 10min in 0.1M triethanolamine (TEA). Sections were then washed in PBS and incubated overnight at 65ºC in hybridization solution [50% formamide, 1X Hybe solution (Sigma-Aldrich), 1mg/ml yeast RNA] containing 1g/ml Dig-labeled riboprobe. After hybridization, sections were washed by immersion in 0.2X saline-Sodium citrate buffer at 72 ºC for 1hr. Dig-labeled probes were detected with an AP-conjugated anti-Dig antibody (Roche) followed by NBT/BCIP (nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate) reaction (Roche). After in situ hybridization, sections were incubated with DAPI for nuclei staining and mounted in Aqua PolyMount (Polysciences) as described previously (Howell et al., 2011) .

Graham L., Kocalis H., Soto I., & Howell G.R. (2020). Deficiency of complement component C1Q prevents cerebrovascular damage and white matter loss in a mouse model of chronic obesity.

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