Anti myosin light chain kinase

Manufactured by Abcam

Anti-myosin light chain kinase is a protein that is involved in the regulation of muscle contraction. It phosphorylates the regulatory light chains of myosin, which is a key step in the activation of muscle contraction.

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Lab products found in correlation

2 protocols using anti myosin light chain kinase

Immunostaining and Imaging of Tumor-Infiltrating T Cells

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Tumor cryo-sections (5 μm) and hydrogels containing T cells were fixed using 4% PFA prior to permeabilization, blocking, and staining with Phalloidin-488 (Life Tech) and anti-myosin light chain kinase (Abcam) for the hydrogels or collagen I (Novus Biologicals) CD8 (Novus Biologicals), and CD3 (Proteintech) for tumors, and DAPI nuclear stain. Secondary anti-Rabbit Alexafluor488 and Alexafluor594 (Thermo Fisher Scientific) as well as ant-rat NL637 (R&D) were used for staining. For immunohistochemical analysis, tissues were dehydrated through graded ethanols followed by antigen retrieval and detection using the ImmPRESS HRP anti-rabbit IgG polymer detection kit (Vector Laboratories). Images were captured using the Zeiss 780 confocal microscope. 3D circularity was calculated using surfaces in Imaris 8.2.1. Second harmonic generation was performed on a Zeiss AxioExaminer microscope equipped with a Coherent Chameleon Visionll multi-photon laser. Number and size of projections were counted in cell tracking videos using FIJI.

Pruitt H.C., Lewis D., Ciccaglione M., Connor S., Smith Q., Hickey J.W., Schneck J.P, & Gerecht S. (2019). Collagen fiber structure guides 3D motility of cytotoxic T lymphocytes. Matrix biology : journal of the International Society for Matrix Biology.

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Synthetic Elastin Peptides for Cell Research

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Synthetic elastin peptides (VGVAPG, AGVPGLGVG, GRKRK and TAMRA-AGVPGLGVG) were purchased from Proteogenix. Mouse anti-MMP-2 and anti-uPA antibodies were from Calbiochem. Y27632, blebbistatin, U0126, PD150606, lactose, chondroitin sulphate, nifedipine and EDTA were from Sigma-Aldrich. EGCG was purchased from Enzo Life Sciences. Rabbit anti-Hsp90, anti-cleaved caspase-3, anti-integrin αV, anti-p-ERM, mouse anti-αvβ3 and anti-αvβ5 integrin antibodies were from Ozyme. Rabbit anti-RPSA, anti-MMP-14, anti-calpain1, anti-ROCK2, anti-myosin light chain kinase, mouse anti-RPSA, anti-RhoA, anti-ROCK1 and mouse IgM isotype control antibodies were purchased from Abcam. Rabbit anti-p-LIMK-and goat anti-cofilin and anti-actin were from Santa Cruz. Anti-integrin αvβ3 antibody was purchased from Millipore. Annexin-5 alexa fluor^® 568, CellTrace Calcein Red-Orange, AM and DiOC18³ were from Invitrogen.

Brassart B., Da Silva J., Donet M., Seurat E., Hague F., Terryn C., Velard F., Michel J., Ouadid-Ahidouch H., Monboisse J.C., Hinek A., Maquart F.X., Ramont L, & Brassart-Pasco S. (2019). Tumour cell blebbing and extracellular vesicle shedding: key role of matrikines and ribosomal protein SA. British Journal of Cancer, 120(4), 453-465.

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