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Apotarget kit

Manufactured by Thermo Fisher Scientific
Sourced in United States

The APOtarget kit is a laboratory equipment product offered by Thermo Fisher Scientific. It is designed for a specific core function, but without further details or interpretation about its intended use.

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5 protocols using apotarget kit

1

Annexin-V Binding Assay for PS Exposure

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Phosphatidylserine (PS) exposure was determined by Annexin-V binding using the APOtarget kit (Invitrogen), according to manufacturer's directions, followed by Flow cytometry [6 (link)].
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2

Phosphatidylserine Exposure Measurement

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Phosphatidylserine (PS) exposure was determined by Annexin-V binding with the APOtarget kit (Invitrogen, Carlsbad, CA, USA), according to manufacturer's directions and analyzed by Flow cytometry or fluorescence microscopy [14 (link), 16 (link)].
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3

Annexin-V Binding Assay for Phosphatidylserine

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Phosphatidylserine exposure was determined by annexin-V binding with the APOtarget kit (Invitrogen) according to manufacturer's directions and analyzed by flow cytometry or fluorescence microscopy.
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4

Apoptosis Assessment by TUNEL and Annexin-V

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DNA fragmentation was determined by the Dead End TM Fluorometric TUNEL kit (Promega), according to manufacturer’s directions. At least ten fields per sample were analyzed under an Olympus BX-53 fluorescence microscope. Phosphatidylserine exposure was determined by Annexin-V binding with the APOtarget kit (Thermo Fisher Scientific), according to manufacturer’s directions, co-stained with PI and analyzed by Flow cytometry on a BD Biosciences (San Jose, CA, USA) FACS Canto Flow Cytometer.
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5

Caspase Activity in Malaria Infection

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Proteins extracted from the lungs of non-infected or P. berghei ANKA-infected DBA/2 mice, and also from cultures of PMLECs from DBA/2 mice (PLMEC) [stimulated or non-stimulated with iRBC or red blood cells (RBC)] were used. The ApoTarget kit (Thermo Scientific) was employed to quantify the aminoacid sequences VDVAD (caspase 2), DEVD (caspase 3), VEID (caspase 6), IETD (caspase 8), and LEHD (caspase 9).
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