Apotarget kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The APOtarget kit is a laboratory equipment product offered by Thermo Fisher Scientific. It is designed for a specific core function, but without further details or interpretation about its intended use.

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Lab products found in correlation

Bx53 fluorescence microscope, by Olympus (1 mentions) Deadend fluorometric tunel kit, by Promega (1 mentions) Facscanto flow cytometer, by BD (1 mentions)

Spelling variants of this product

ApoTarget™Annexin-V FITC Apoptosis kit (8 citations) ApoTarget caspase colorimetric protease assay sampler kit (6 citations) ApoTarget (3 citations) ApoTarget Caspase-3 Protease Activity Kit (2 citations) ApoTarget™ Caspase-3 Colorimetric Protease Assay kit (2 citations) ApoTarget caspase colorimetric protease assay sample kit (2 citations) ApoTarget™ Caspase Colorimetric Protease Assay Kit (2 citations)

5 protocols using apotarget kit

Annexin-V Binding Assay for PS Exposure

Cited 1 time

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Phosphatidylserine (PS) exposure was determined by Annexin-V binding using the APOtarget kit (Invitrogen), according to manufacturer's directions, followed by Flow cytometry [6 (link)].

Lobos-González L., Silva V., Araya M., Restovic F., Echenique J., Oliveira-Cruz L., Fitzpatrick C., Briones M., Villegas J., Villota C., Vidaurre S., Borgna V., Socias M., Valenzuela S., Lopez C., Socias T., Varas M., Díaz J., Burzio L.O, & Burzio V.A. (2016). Targeting antisense mitochondrial ncRNAs inhibits murine melanoma tumor growth and metastasis through reduction in survival and invasion factors. Oncotarget, 7(36), 58331-58350.

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Phosphatidylserine Exposure Measurement

Cited 2 times

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Phosphatidylserine (PS) exposure was determined by Annexin-V binding with the APOtarget kit (Invitrogen, Carlsbad, CA, USA), according to manufacturer's directions and analyzed by Flow cytometry or fluorescence microscopy [14 (link), 16 (link)].

Borgna V., Villegas J., Burzio V.A., Belmar S., Araya M., Jeldes E., Lobos-González L., Silva V., Villota C., Oliveira-Cruz L., Lopez C., Socias T., Castillo O, & Burzio L.O. (2017). Mitochondrial ASncmtRNA-1 and ASncmtRNA-2 as potent targets to inhibit tumor growth and metastasis in the RenCa murine renal adenocarcinoma model. Oncotarget, 8(27), 43692-43708.

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Annexin-V Binding Assay for Phosphatidylserine

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Phosphatidylserine exposure was determined by annexin-V binding with the APOtarget kit (Invitrogen) according to manufacturer's directions and analyzed by flow cytometry or fluorescence microscopy.

Vidaurre S., Fitzpatrick C., Burzio V.A., Briones M., Villota C., Villegas J., Echenique J., Oliveira-Cruz L., Araya M., Borgna V., Socías T., Lopez C., Avila R, & Burzio L.O. (2014). Down-regulation of the Antisense Mitochondrial Non-coding RNAs (ncRNAs) Is a Unique Vulnerability of Cancer Cells and a Potential Target for Cancer Therapy. The Journal of Biological Chemistry, 289(39), 27182-27198.

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Apoptosis Assessment by TUNEL and Annexin-V

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DNA fragmentation was determined by the Dead End TM Fluorometric TUNEL kit (Promega), according to manufacturer’s directions. At least ten fields per sample were analyzed under an Olympus BX-53 fluorescence microscope. Phosphatidylserine exposure was determined by Annexin-V binding with the APOtarget kit (Thermo Fisher Scientific), according to manufacturer’s directions, co-stained with PI and analyzed by Flow cytometry on a BD Biosciences (San Jose, CA, USA) FACS Canto Flow Cytometer.

Fitzpatrick C., Bendek M.F., Briones M., Farfán N., Silva V.A., Nardocci G., Montecino M., Boland A., Deleuze J.F., Villegas J., Villota C., Silva V., Lobos-Gonzalez L., Borgna V., Barrey E., Burzio L.O, & Burzio V.A. (2019). Mitochondrial ncRNA targeting induces cell cycle arrest and tumor growth inhibition of MDA-MB-231 breast cancer cells through reduction of key cell cycle progression factors. Cell Death & Disease, 10(6), 423.

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Caspase Activity in Malaria Infection

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Proteins extracted from the lungs of non-infected or P. berghei ANKA-infected DBA/2 mice, and also from cultures of PMLECs from DBA/2 mice (PLMEC) [stimulated or non-stimulated with iRBC or red blood cells (RBC)] were used. The ApoTarget kit (Thermo Scientific) was employed to quantify the aminoacid sequences VDVAD (caspase 2), DEVD (caspase 3), VEID (caspase 6), IETD (caspase 8), and LEHD (caspase 9).

Sercundes M.K., Ortolan L.S., da Silva Julio V., Bella L.M., de Castro Quirino T., Debone D., Carneiro-Ramos M.S., Christoffolete M.A., Martins J.O., D’Império Lima M.R., Alvarez J.M., Amarante-Mendes G.P., Gonçalves L.A., Marinho C.R, & Epiphanio S. (2022). Blockade of caspase cascade overcomes malaria-associated acute respiratory distress syndrome in mice. Cell Death & Disease, 13(2), 144.

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