RAW 264.7 murine macrophage cells (ATCC®TIB-71™) and Nrf2-luciferase reporter MCF7 stable cells (SL-0010) were purchased from American Type Culture Collection (ATCC, Mannasas, VA, USA) and Signosis, Inc. (Santa Clara, CA, USA), respectively. Solid-phase extraction (SPE) was carried out using pre-packed Strata® SPE-C18 (1 g/6 mL, 55 µm) cartridges (Phenomenex, Torrance, CA, USA). Reversed-phase high performance liquid chromatography (RP-HPLC) was performed using Shimadzu Prominence LC-20AT equipped with an SPD-M20A photo-diode array (PDA) detector and Phenomenex Synergi Hydro-RP column (4 µm, 80 Å, 250 × 10 mm). Low resolution mass analysis was carried out on a Shimadzu LCMS-8040 triple quadrupole mass spectrometer equipped with electrospray ionization (ESI) ion source detector using a linear gradient 5–100% aq. CH3CN with 0.1% (v/v) formic acid. High resolution mass analysis was carried out on Waters Xevo® G2-XS Quadrupole Time-of-Flight (QToF) mass spectrometer equipped with ESI ion source detector using a linear gradient 5–100% aq. CH3CN with 0.1% (v/v) formic acid. The 1D- and 2D-NMR spectra were recorded on a Varian 500 MHz NMR equipped with a 3 mm probe. Statistical analyses were carried using GraphPad Prism version 9.3.1 for Windows, GraphPad Prism Software (San Diego, CA, USA).
500 mhz nmr
Manufactured by Agilent Technologies
Sourced in United States
The 500 MHz NMR is a nuclear magnetic resonance spectrometer that operates at a frequency of 500 MHz. It is designed to analyze the molecular structure of chemical compounds by detecting the magnetic properties of their nuclei.
Automatically generated - may contain errors
Lab products found in correlation
6510 q tof lc ms, by Agilent Technologies (2 mentions)
Xevo g2 xs quadrupole time of flight q tof mass spectrometer, by Waters Corporation (1 mentions)
Synergi hydro rp, by Phenomenex (1 mentions)
Lcms 8040 triple quadrupole mass spectrometer, by Shimadzu (1 mentions)
Atcc tib 71, by American Type Culture Collection (1 mentions)
Dsc823e, by Mettler Toledo (1 mentions)
1260 infinity quaternary lc apparatus, by Agilent Technologies (1 mentions)
Unity inova spectrometer, by Agilent Technologies (1 mentions)
P 200 polarimeter, by Jasco (1 mentions)
J 715 spectrophotometer, by Jasco (1 mentions)
Triple tof 4600 mass spectrometer, by AB Sciex (1 mentions)
Du800 spectrophotometer, by Beckman Coulter (1 mentions)
Ultimate 3000 hplc system, by Thermo Fisher Scientific (1 mentions)
Acquity uplc h class, by Waters Corporation (1 mentions)
Alanine, by Merck Group (1 mentions)
Phenylalanine, by Merck Group (1 mentions)
9 protocols using 500 mhz nmr
1
Analytical Characterization of Bioactive Compounds
2
Quantifying Residual PEG in Fiber Mats
3
NMR Analysis of Organophosphorus Compounds
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The NMR samples were
prepared under a purified N2 atmosphere with dried, oxygen-free
THF.90 For 31P NMR, nondeuterated
THF90 was used while disabling the lock
function. The 13C and 31P NMR measurements were
performed with routine pulse programs and proton decoupling. The samples
were measured on a Varian 500 MHz NMR with the external standard ClPPh2 (neat liquid, δ(31P) = +81.92 ppm) for 31P and the solvent signals for 13C. The 31P NMR spectra were typically obtained with satisfactory S/N ratio
with 128 or 256 scans. Line broadening factors of up to 10 Hz were
applied as needed.
prepared under a purified N2 atmosphere with dried, oxygen-free
THF.90 For 31P NMR, nondeuterated
THF90 was used while disabling the lock
function. The 13C and 31P NMR measurements were
performed with routine pulse programs and proton decoupling. The samples
were measured on a Varian 500 MHz NMR with the external standard ClPPh2 (neat liquid, δ(31P) = +81.92 ppm) for 31P and the solvent signals for 13C. The 31P NMR spectra were typically obtained with satisfactory S/N ratio
with 128 or 256 scans. Line broadening factors of up to 10 Hz were
applied as needed.
4
Synthesis of Metal Salt Solutions
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Chemicals or metal salts were purchased from Sigma Aldrich, Acros organics, Alfa Aeser or LPS solution and used without further purification unless described. 1H-NMR spectra were collected with Varian 500 MHz NMR using CDCl3 or CD3OD solvents. All metal salts were prepared by dissolving metal chloride (MnCl2, CoCl2·6H2O, NiCl2·6H2O, CuCl2·2H2O, ZnCl2, NaCl, LiCl) in ddH2O. All aqueous solutions were prepared with ddH2O and filtered with 0.22 μm syringe-filters before usage.
5
Characterization of Conulothiazoles via NMR
6
NMR Characterization of TNT-APTES Complexes
7
Ammonium Bicarbonate Buffers for Reverse Phase Chromatography
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Ammonium bicarbonate buffers used for analytical and preparative reverse phase chromatography were prepared and adjusted to pH 7 upon addition of the appropriate amount of acetic acid. All compounds purified by reversed phase chromatography were lyophilized to remove buffer and acetonitrile. Compounds were lyophilized for no fewer than two days. Reactions were monitored with a Waters Acquity H Class UPLC equipped with PDA detector and SQ mass detector using a Waters UPLC BEH C18 1.7 μm, 2.1 X 50 mm column. Reverse phase preparative chromatography was performed on a Biotage Isolera One utilizing SNAP Ultra C18 columns. 1H NMR were obtained on an Agilent 500 MHz NMR. All experiments were conducted at 25°C.
8
Synthesis and Characterization of Ester-Protected Amino Acids
9
Synthesis and Characterization of Monounsaturated Fatty Acids
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(Z)-hexadec-13-enoic acid and (Z)-octadec-15-enoic acid were prepared by our previously reported procedures 39, 40 . (E)-octadec-9-enoic acid was purchased from Chemsupply (Gillman, SA, Australia). (Z)-hexadec-9-enoic acid, (Z)-octadec-11-enoic acid, (E)-octadec-11-enoic acid, (Z)-octadec-6-enoic acid and all other reagents and anhydrous solvents were purchased from Sigma Aldrich (Castle Hill, NSW, Australia).
Non-commercially available MUFAs were synthesised as described in the supporting information. The purity of all test compounds (3 -20) was confirmed by elemental analysis carried out in the Campbell Microanalytical Laboratory at the Department of Chemistry, University of Otago. All values were within ±0.4% of the calculated values.
Dry Column Vacuum Chromatography (DCVC) was used to purify reaction products on silica gel with gradient elutions. TLC was performed on silica gel 60 F254 plates. 1 H and 13 C NMR spectra were recorded on an Agilent 500 MHz NMR. Spectra were referenced internally to residual solvent (CDCl3; 1 H 7.26, 13 C 77.10. d6-DMSO; 1 H 2.49, 13 C 39.52). High resolution mass spectra were recorded on Agilent 6510 Q-TOF LC/MS.
Non-commercially available MUFAs were synthesised as described in the supporting information. The purity of all test compounds (3 -20) was confirmed by elemental analysis carried out in the Campbell Microanalytical Laboratory at the Department of Chemistry, University of Otago. All values were within ±0.4% of the calculated values.
Dry Column Vacuum Chromatography (DCVC) was used to purify reaction products on silica gel with gradient elutions. TLC was performed on silica gel 60 F254 plates. 1 H and 13 C NMR spectra were recorded on an Agilent 500 MHz NMR. Spectra were referenced internally to residual solvent (CDCl3; 1 H 7.26, 13 C 77.10. d6-DMSO; 1 H 2.49, 13 C 39.52). High resolution mass spectra were recorded on Agilent 6510 Q-TOF LC/MS.
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