Neurons were seeded at a density of 5 × 103 cells/well in a 96-well plate. After the neurons were incubated with drugs following the above procedures, cell viability was detected using Cell counting kit-8 (CCK-8) reagent (Dalian Meilun Biotechnology Co. Ltd., #MA0218, Dalian, China). Absorbance was obtained on a microplate reader at 450 nm. Cell viability was calculated according to the formula provided by the kit.
Cell counting kit 8 cck 8 reagent
Manufactured by Meilun
Sourced in China
Cell Counting Kit-8 (CCK-8) is a reagent used for quantifying the number of viable cells in a sample. It utilizes a water-soluble tetrazolium salt to detect the metabolic activity of cells, which is proportional to the number of viable cells present.
Automatically generated - may contain errors
Lab products found in correlation
El800, by Agilent Technologies (1 mentions)
Multimode microplate reader, by Agilent Technologies (1 mentions)
Apc conjugated anti mouse f4 80, by Thermo Fisher Scientific (1 mentions)
Anti mouse cd16 32, by Thermo Fisher Scientific (1 mentions)
Fitc conjugated anti mouse cd86, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti mouse cd206, by Thermo Fisher Scientific (1 mentions)
Lipopolysaccharide lps, by Santa Cruz Biotechnology (1 mentions)
4 protocols using cell counting kit 8 cck 8 reagent
1
Cell Viability Assay for Neurons
2
Evaluating H9c2 Cell Viability
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H9c2 cardiomyocytes (5×103 cells/well) were seeded in 96-well plates, and each well was supplemented with 100 µL medium. After the cells adhered to the wall, they were treated according to the experimental requirements. After 24 hours, fresh medium was replaced, and 10 µL Cell Counting Kit-8 (CCK8) reagent (Meilunbio, Dalian, China) was added to each well. At the same time, blank control wells (100 µL cell medium, 10 µL CCK8 solution, no cells) were set up and incubated at 37 °C for 2 hours. The absorbance value of each well was measured by a microplate reader (El × 800, BioTek, USA) at OD450 nm. Cell viability % was calculated as (OD of cells in experimental wells -OD of blank wells)/(OD of control wells -OD of blank wells) × 100%. Results were repeated six times for each experiment.
3
Cell Proliferation Assay Using CCK-8 Reagent
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A total of 3 × 103 transfected cells were harvested and plated in 100 μl of medium containing 10% FBS per well in 96-well plates. Then, 100 μl of medium containing 10% Cell Counting Kit-8 (CCK-8) reagent (Meilunbio, Dalian, China) was added to each well at different time points (0, 24, 48, 72 or 96 h). Then, the cells were incubated at 37°C for 1 h. The absorbance at 450 nm was measured by a multimode microplate reader (BioTek, Bedfordshire, UK).
4
Multifunctional Nutrient Combination Effects
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Ginseng extract (Ginsenosides ≥80%, designated as R) and EGCG (designated as E) with purity higher that 94% were purchased from Shanghai Novanat Co., Ltd. (Shanghai, China). PDX (designated as D) with purity higher that 90% was purchased from Tate & Lyle Trading Co., Ltd. (Shanghai, China). The complex of selected nutrients was proposed based on the experimental design and designated as the combination of the abbreviation of the corresponding nutrient (ERD). Lipopolysaccharide (LPS) was purchased from Santa Cruz Biotechnology (California, United States). Cell Counting Kit-8 (CCK-8) reagent was purchased from Meilunbio (Dalian, China). Anti-mouse CD16/32, APC-conjugated anti-mouse F4/80, FITC-conjugated anti-mouse CD86, and PE-conjugated anti-mouse CD206 antibodies were purchased from Thermo Fisher Scientific (Waltham, MA, United States) for flow cytometry analysis. Recombinant mouse IFN-γ and IL-4 were provided by Thermo Fisher Scientific. Enzyme-linked immunosorbent assay (ELISA) kits for TNF-α and IL-1β analysis were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Neutral red was from sourced from Aladdin (Shanghai, China). An apoptosis kit was purchased from Thermo Fisher Scientific. All reagents used in this study were of analytical grade unless otherwise indicated.
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