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Gata4

Manufactured by Proteintech
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GATA4 is a monoclonal antibody that recognizes the GATA4 protein, a transcription factor involved in the regulation of gene expression. This antibody can be used for various applications, including Western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

Ring1b, by Cell Signaling Technology (2 mentions) Pierce crosslink magnetic ip co ip kit, by Thermo Fisher Scientific (1 mentions) Anti ubiquitin, by Cell Signaling Technology (1 mentions) His tag, by Cell Signaling Technology (1 mentions) Ha tag, by Cell Signaling Technology (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions) Ecl plus detection system, by GE Healthcare (1 mentions) Nf κb p65, by Cell Signaling Technology (1 mentions) Il 1β, by Santa Cruz Biotechnology (1 mentions) γh2ax, by Cell Signaling Technology (1 mentions) 8 ohdg, by Abcam (1 mentions) Nbp1 19532, by Novus Biologicals (1 mentions) Interleukin 6 (il 6), by Santa Cruz Biotechnology (1 mentions) Nf κb p65, by Santa Cruz Biotechnology (1 mentions) P ulk1 ser757, by Cell Signaling Technology (1 mentions) β actin, by Bioworld Technology (1 mentions) P p65 ser536, by Abcam (1 mentions) Hsc70, by Proteintech (1 mentions) Ang 2, by Novus Biologicals (1 mentions) Lamp2, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Anti-GATA4 (4 citations)

7 protocols using gata4

1

Crosslinking Protein Immunoprecipitation Workflow

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Total proteins were used for immunoprecipitation using Pierce™ Crosslink Magnetic IP/Co‐IP Kits (#88805, Thermo Scientific Pierce™ Crosslink Magnetic IP/Co‐IP Kit, Thermo Fisher Scientific, IL, USA) as recommended by the supplier. Primary antibodies against Bmi‐1 (#5856, Cell Signaling Technology, USA), RING1B (#5694, Cell Signaling Technology, USA), GATA4 (#19530, Proteintech, USA), p62 (#39749, Cell Signaling Technology, USA), anti‐ubiquitin (#91112, Cell Signaling Technology, USA), HSC70 (10654‐1‐AP, Proteintech, USA), DYKDDDDK Tag (Anti‐FLAG M2 antibody, #14793, Cell Signaling Technology, USA), His‐Tag (#12698, Cell Signaling Technology, USA), HA‐Tag (#3724, Cell Signaling Technology, USA) and Myc‐Tag (M1405‐5, Hangzhou HuaAn Biotechnology, China) were used.
Chen H., Zhou J., Chen H., Liang J., Xie C., Gu X., Wang R., Mao Z., Zhang Y., Li Q., Zuo G., Miao D, & Jin J. (2022). Bmi‐1‐RING1B prevents GATA4‐dependent senescence‐associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4. Clinical and Translational Medicine, 12(4), e574.
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2

Protein Expression Analysis by Western Blot

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Protein extracts (40 μg) were separated using 12% (wt./vol.) SDS-PAGE, transferred onto a polyvinylidene fluoride (PVDF) membrane, and probed with antibodies for atrial natriuretic peptide (ANP) and β-myosin heavy chain (β-MHC; Abcam), GATA4 (Proteintech, Chicago, IL, USA), p-NF-кB p65, NF-кB p65 (Cell Signaling Technology, Beverly, MA, USA) overnight at 4°C. Membranes were then washed extensively with TBS/T and incubated with a horseradish peroxidase-conjugated secondary antibody (Santa Cruz) for 1 h at room temperature. Protein was visualized using the ECL Plus detection system (GE Healthcare, WI, USA).
Li H., Xu J.D., Fang X.H., Zhu J.N., Yang J., Pan R., Yuan S.J., Zeng N., Yang Z.Z., Yang H., Wang X.P., Duan J.Z., Wang S., Luo J.F., Wu S.L, & Shan Z.X. (2019). Circular RNA circRNA_000203 aggravates cardiac hypertrophy via suppressing miR-26b-5p and miR-140-3p binding to Gata4. Cardiovascular Research, 116(7), 1323-1334.
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3

Comprehensive Cardiac Biomarker Analysis

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Primary antibodies were against ANP (#AB5490, Millipore, USA; sc‐515701, Santa Cruz Biotechnology Inc., USA), BNP (#DF6902, Affinity Biosciences, USA; ab19645, Abcam, USA), GATA4 (#19530, Proteintech, USA), 8‐OHdG (ab62623, Abcam, USA), γH2A.X (#9718, Cell Signaling Technology, USA), NF‐κB‐p65 (#8242, Cell Signaling Technology, USA), IL‐1β (sc‐52012, Santa Cruz Biotechnology Inc., USA), IL‐6 (sc‐1265, Santa Cruz Biotechnology Inc., USA) or TNF‐α (NBP1‐19532, Novus Biologicals, USA).
Chen H., Zhou J., Chen H., Liang J., Xie C., Gu X., Wang R., Mao Z., Zhang Y., Li Q., Zuo G., Miao D, & Jin J. (2022). Bmi‐1‐RING1B prevents GATA4‐dependent senescence‐associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4. Clinical and Translational Medicine, 12(4), e574.
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4

Comprehensive Protein Analysis in Cardiac Cells

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Primary antibodies were against p19 (NB200‐106, Novus Biologicals, CO, USA), p21 (#2947, Cell Signaling Technology, Beverly, MA, USA), p53 (#2424, Cell Signaling Technology, USA), p16 (ab211542, Abcam, Cambridge, MA, USA), renin (#5250, Cell Signaling Technology, USA), Ang II (NBP1‐31127, Novus Biologicals, USA), ANP (#AB5490, Millipore, MA, USA; sc‐515701, Santa Cruz Biotechnology Inc., Dallas, TX, USA, USA), BNP (#DF6902, Affinity Biosciences, OH, USA; ab19645, Abcam, USA), GATA4 (#19530, Proteintech, IL, USA; sc‐25310, Santa Cruz Biotechnology Inc., USA), LC3B (#NB600‐1384, Novus Biologicals, USA), p62 (#39749, Cell Signaling Technology, USA), Bmi‐1 (#5856, Cell Signaling Technology, USA; 66161‐1‐Ig, Proteintech, USA), RING1B (#5694, Cell Signaling Technology), NF‐κB‐p65 (sc‐8008, Santa Cruz Biotechnology Inc., USA; #8242, Cell Signaling Technology, USA), p‐p65 (Ser536) (ab76302, Abcam, USA), IκB‐α (AF1282, Beyotime Biotechnology, Shanghai, China), p‐IκB‐α (Ser32) (sc‐8404, Santa Cruz Biotechnology Inc., USA), p‐Chk2 (Thr68) (PA5‐104715, Invitrogen Inc. CA, USA), SOD‐2 (NB100‐1992, Novus Biologicals, USA), HSC70 (10654‐1‐AP, Proteintech, USA), p‐ULK1 (Ser757) (#14202, Cell Signaling Technology, USA) and ULK1 (sc‐390904, Santa Cruz Biotechnology, USA). β‐actin (AP0060, Bioworld Technology Inc., MN, USA) was loading control for total protein.
Chen H., Zhou J., Chen H., Liang J., Xie C., Gu X., Wang R., Mao Z., Zhang Y., Li Q., Zuo G., Miao D, & Jin J. (2022). Bmi‐1‐RING1B prevents GATA4‐dependent senescence‐associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4. Clinical and Translational Medicine, 12(4), e574.
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5

Immunodetection of Key Cellular Markers

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Primary antibodies were against GATA4 (#19530, Proteintech, USA), GATA4 (sc‐25310, Santa Cruz Biotechnology Inc., USA), LC3B (#NB600‐1384, Novus Biologicals, USA), p62 (#39749, Cell Signaling Technology, USA) or LAMP2 (sc‐20004, Santa Cruz Biotechnology Inc., USA).
Chen H., Zhou J., Chen H., Liang J., Xie C., Gu X., Wang R., Mao Z., Zhang Y., Li Q., Zuo G., Miao D, & Jin J. (2022). Bmi‐1‐RING1B prevents GATA4‐dependent senescence‐associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4. Clinical and Translational Medicine, 12(4), e574.
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6

Pathways and Markers in Stem Cell Differentiation

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The Wnt pathway inhibitors MSAB and Wnt agonist 1, the TGF-β agonist SRI-011381, and the TGF-β1 inhibitor pirfenidone (PFD) were obtained from Selleck (Shanghai, China). Antibodies against collagen I, CD9, and CD63 were obtained from Beyotime (Shanghai, China). Antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH), collagen II, GATA4, aggrecan (Acan), tumor susceptibility gene 101 (TSG101), and TGF-β1 were purchased from Proteintech (Wuhan, China). Collagenase II was purchased from Sangon Biotech (Shanghai, China). Antibodies against a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5), sirtuin 9 (SOX9), transcription factor 4, and β-catenin were purchased from Bioss (Beijing, China). Antibodies against HIF-1α were obtained from Santa Cruz Biotechnology (Dallas, TX, United States). PKH26 was obtained from Sigma (St. Louis, MO, United States). MSC osteogenic differentiation medium, chondrogenic differentiation medium, and adipogenic differentiation medium were provided by Cyagen (Guangzhou, China).
Luo L., Gong J., Zhang H., Qin J., Li C., Zhang J., Tang Y., Zhang Y., Chen J., Zhou Y., Tian Z., Liu Y, & Liu M. (2021). Cartilage Endplate Stem Cells Transdifferentiate Into Nucleus Pulposus Cells via Autocrine Exosomes. Frontiers in Cell and Developmental Biology, 9, 648201.
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7

Quantifying Cardiac Stem Cells in Myocardium

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Paraffin sections (~4 μm thickness) of the myocardial tissue including anatomical areas were immunostained against c-kit (Cell Signaling Technology, Danvers, MA), CD-133 (Life Technologies, Grand Island, NY), Nkx2.5 (Abcam, Boston, MA), cardiac troponin I (Abcam, Boston, MA) and GATA4 (Proteintech, Chicago, IL) and examined using Leica SP2 inverted confocal microscopy system (Leica Microsystems, Wetzlar, Germany) and LSM 710 confocal microscopy system (Carl Zeiss, Oberkochen, Germany). Images were measured by Image J (provided by NIH, http://imagej.nih.gov/ij/). The color threshold was adjusted manually and positive signal intensity was analyzed. The number of LV cardiomyocytes and CSCs per unit volume of myocardium was determined using Velocity 3D image analysis software (PerkinElmer, Waltham, MA).
Chung W.J., Cho A., Byun K., Moon J., Ge X., Seo H.S., Moon E., Dash R, & Yang P.C. (2016). Apelin-13 infusion salvages the peri-infarct region to preserve cardiac function after severe myocardial injury. International journal of cardiology, 222, 361-367.
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