Aorta images were recorded with a stereomicroscope-dedicated camera (IC80 HD camera, MZ6 microscope, Leica Microsystems, Germany) and analysed using ImageJ image processing program (http://rsb.info.nih.gov/ij/ ). An operator blinded to dietary treatment quantified the atherosclerotic plaques.
Mz6 microscope
Manufactured by Leica
Sourced in Germany
The Leica MZ6 is a stereomicroscope designed for versatile laboratory applications. It features a binocular observation tube, providing a comfortable viewing experience. The MZ6 offers a magnification range from 6.3x to 40x, allowing users to examine a wide variety of specimens with precision. The microscope's optical system ensures high-quality imaging and clarity. The MZ6 is a reliable and durable instrument suitable for various laboratory tasks.
Automatically generated - may contain errors
Lab products found in correlation
Ic80 hd camera, by Leica (2 mentions)
Axio imager, by Zeiss (2 mentions)
Dfc425, by Leica (1 mentions)
Axiocam 506, by Zeiss (1 mentions)
Dfc420, by Leica (1 mentions)
Dpx mountant, by Merck Group (1 mentions)
Extravidin, by Merck Group (1 mentions)
Dmrb light microscope, by Leica (1 mentions)
E2886, by Merck Group (1 mentions)
Sudan 4, by Merck Group (1 mentions)
20 protocols using mz6 microscope
1
Quantifying Aortic Atherosclerosis via Microscopy
2
Aorta Atherosclerosis Quantification
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Aorta images were captured with a stereomicroscope-dedicated camera (IC80 HD camera, MZ6 microscope, Leica Microsystems, Germany), and analysed with ImageJ image processing program [17] (link). Atherosclerosis extent was quantified by two independent operators blinded to the dietary treatments.
3
Cardiac Morphometric Analysis in Mice
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The MFS and WT mice were sacrificed by means of CO2-inhalation using gradual fill (1.0 L/min) at the age of 1, 2, and 3 months. A thoracotomy was executed in order to isolate the heart, which was subsequently flushed with a cooled 30 mM KCl solution to induce cardioplegia. After KCl-flushing the total heart weight was measured and macroscopic pictures were taken using a Leica MZ6 microscope (Leica Microsystems, Wetzlar, Germany) with a DFC425 color camera (Leica Microsystems) in order to analyze the heart length and width. For further histological analysis, the hearts were flushed and fixed for 20 h at 4 °C with 4% paraformaldehyde. After paraformaldehyde fixation, the hearts were stored in a 70% ethanol solution until further processing. Furthermore, the left tibia was amputated, and soft tissue was removed in order to obtain the tibia length, which was used for normalization of the heart weights. Heart weight correlates with tibia length and is less prone to fluctuation as observed for body weight. Furthermore, due to bony overgrowth the body- and tibia-length of the MFS mice was higher compared to the WT mice. For protein analysis, the left ventricle of the heart was isolated and stored at –80 °C until further processing.
4
Barnacle Larval Settlement Assay
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The direct antilarval-settlement assay was conducted using cyprids of the barnacle B. amphitrite Darwin as described previously [30 (link),31 (link),32 (link),33 (link)]. In brief, adult B. amphitrite (Darwin) were collected from the intertidal zone in Pak Sha Wan, Hong Kong (22°19′ N, 114°16′ E) and raised to competence for experiments. Elasnin was dissolved in DMSO and diluted into four concentrations from 50 to 6.25 μg/mL with a twofold serial dilution. DMSO was used as a negative control. About 10 ± 2 B. amphitrite cyprids were inoculated into each well of a 24-well polystyrene culture plate that contained 2 mL of 0.22 μm FSW with different treatments. For all treatments and controls, three replicates were performed. The plates were then incubated at 25 °C in darkness. After 24 and 48 h, the number of settled and swimming larvae was counted using a Leica MZ6 microscope (Leica Microsystems, Wetzlar, Germany), and possible toxic effects were also noted.
5
Colony Formation Assay with Inhibitor
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Cells were plated in triplicates in 10% FBS complete RPMI medium with 1% methylcellulose containing the indicated concentration of inhibitor. Colonies were counted after 2 weeks using a Leica MZ 6 microscope.
6
Scoring Seed Development in Siliques
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Mature siliques were dissected with fine tweezers under a Leica MZ6 microscope, and the F2 seeds from 15 siliques (five from each of three individual plants) were scored for numbers of normally developing or aborted seeds (N, A) and for the total number of ovules whether fertilized or not (T). These were expressed as both proportions of the total ovule number (%N = N/T; %A = A/T) and proportions of the number of ovules which had been fertilized (N/(N + A), A/(N + A)) to distinguish between total plant fecundity and the post-fertilization fate of the F2 seeds specifically.
7
Heat Shock Impacts Nematode Fecundity
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Animals were heat‐shocked as L4 at 35 °C for 1 h and immediately transferred back to 20 °C onto RNAi plates. The adult animals were transferred onto fresh plates every day until day 7 of life, and the number of eggs was counted for each animal over this period of time using a Leica MZ6 microscope. Ten animals were analyzed in parallel with one nematode per plate.
8
Histological Analysis of Hif-p4h-2 Brains
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The Hif-p4h-2gt/gt brains were cut into four ~ 2 mm sections along the coronal plate, fixed in 0.2% glutaraldehyde (GA) for 2 h and stained with 2 mg/ml 5-bromo-4-chloro-3indolyl-b-d-galactopyranoside (X-gal). The stained tissues were viewed with a Leica MZ6 microscope and photographed with Leica DFC425 camera. To detect β-gal activity histologically, the brain sections were fixed in 0.2% GA 3 × 2 h, immersed overnight in 30% sucrose frozen in cryo blocks, 25 µm cryo-cut sections were floating stained in 2 mg/ml X-gal and viewed with Zeiss Axio Imager motorized histology microscope and photographed with Axiocam 506 color camera.
9
Quantitative Analysis of Muscle Fibers
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Serial transverse 10-μm-thick sections of muscle tissues were stained with H&E and intracytoplasmic lipids were stained with oil red O (oil O staining) according to previously published procedures [70 ]. Please note that the samples were selected from the same cohort for metabolome detection; the corresponding sample numbers were the same as samples used for metabolic tests (n = 50). A total of 200–400 fibers of white muscle per fish were studied using a Leica MZ 6 microscope for their cross-sectional area, and the diameter (d = 2r) of each fiber was calculated from the fiber area (A) (A = π·r2), thus, d = 2*√ (A·π−1)). A size limit for identifying fibers was set at fiber diameters ≥10 μm as the optical resolution below this limit did not allow for sufficient identification and accuracy in the analyses [71 ]. The circularity of each fiber was also determined. The free software Image J [72 ] was used for quantitative statistics and analyses.
10
Hydrocephalus in H-Tx Rat Model
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All experiments were sanctioned by the Home Office Animals (Scientific Procedures) Act Inspectorate (UK) and were carried out under project licence PPL70/8025. Colonies of SD (control group) and H-Tx rats (experimental group) were kept on a 12 h light 12 h dark cycle commencing at 8 am, at a constant temperature, humidity and filtered air, with free access to food and water and low light and sound levels. The H-Tx colony was maintained through brother-sister mating between unaffected animals, and the SD colony was maintained through random pair mating. The animals were fed the standard Beekay rat and mouse diet no. 2 (B and K Universal, Hull, UK). H-Tx foetuses were categorised as either affected or unaffected H-Tx based on the excessive CSF accumulation which showed as a gross dooming of the head of affected individuals under a Leica MZ6 microscope (Milton Keynes, UK). Sexually mature unaffected H-Tx and SD males were used for the study.
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