Galaxie chromatography data system 1

The moisture of minced flesh was determined in accordance with AOAC method [37 ]. The total lipids were obtained according to Folch et al. [38 (link)] method, then they were gravimetrically quantified. The fatty acids (FAs) were determined in the lipid extract after trans-esterification to methyl esters (FAME), using a base-catalyzed trans-esterification [39 (link)]. The FA composition was determined by gas chromatography (GC) using a Varian GC 430 gas chromatograph (Varian Inc., Palo Alto, CA, USA), equipped with a flame ionization detector (FID) and a Supelco Omegawax™ 320 m capillary column (Supelco, Bellefonte, PA, USA). The GC conditions were recovered from Secci et al. [40 (link)]. Chromatograms were recorded with the Galaxie Chromatography Data System 1.9.302.952 (Varian Inc., Palo Alto, CA, USA). FAs were identified by comparing the FAME retention time with those of the Supelco 37 component FAME mix standard (Supelco, Bellefonte, PA, USA) and quantified through calibration curves, using tricosanoic acid (C23:0) (Supelco, Bellefonte, PA, USA) as internal standard.

Total lipids content was determined using the method of Folch et al.^{53 (link)}; fatty acid profile of total lipids, using the modified technique of Morrison and Smith^{54 (link)}.Fatty acids (FAs) methyl esters were analyzed by gas chromatography using a Varian 430 apparatus (Varian Inc., Palo Alto, CA, USA) equipped with a flame ionisation detector. FAs separation occurred in a Supelco Omegawax TM 320 capillary column (30-mlength; 0.32 mm internal diameter; 0.25 lm film thick-ness; Supelco, Bellafonte, PA, USA). The chromato-graphic conditions were an initial temperature of 160 C, which was then increased by 2 C/min until the temperature reached 220 C. One microliter of sample in hexane was injected with the carrier gas (helium) at a constant flow of 1.5 mL min⁻¹ and at a split ratio of 1:20. The detector temperature was set at 260 C. The chromatograms were recorded using computing integrator software (Galaxie Chromatography Data System 1.9.302.952; Varian Inc.). The percentage of each fatty acid was calculated on the total of fatty acids detected and expressed as g/100 g of FAMEs. Fatty acid groups were obtained as sum of all saturated fatty acids (SFA) detected, sum of all monounsaturated fatty acids (MUFA) and sum of all polyunsaturated fatty acids (PUFA).