Dissected ear and tassel primordia were fixed overnight in FAA (4% formalin, 50% ethanol, 5% acetic acid, and 0.01% Triton X-100), dehydrated through an ethanol series, and transitioned to 100% acetone before drying using a 931.GL Supercritical Autosamdri critical point dryer (Tousimis, Maryland) according to the manufacturer’s protocol. Dried samples were mounted on stubs and sputter-coated with gold:palladium (80:20) with Quorum Q150TES (Quorum Technologies, East Sussex, England), before imaging on a XL30 FEI scanning electron microscope (TSS Microscopy, Hillsboro, OR, USA) with an acceleration voltage of 5 to 30 kV under high vacuum mode (<20 mbar) with a working distance of 10 to 20 mm.
Quorum q150t es
Manufactured by Quorum Technologies
Sourced in United Kingdom, Germany
The Quorum Q150T ES is a compact, tabletop sputter coater designed for coating small samples. It features a motorized stage and a high-vacuum pumping system to ensure consistent and reliable coating results.
Automatically generated - may contain errors
Lab products found in correlation
Sigma 300, by Zeiss (2 mentions)
Merlin, by Zeiss (2 mentions)
Jsm 6010la, by JEOL (2 mentions)
Fei quanta 200, by Thermo Fisher Scientific (2 mentions)
Spectrum two, by PerkinElmer (1 mentions)
Ultra 55 field emission scanning electron microscope, by Zeiss (1 mentions)
Em cpd030, by Leica (1 mentions)
Quanta 250 feg, by Thermo Fisher Scientific (1 mentions)
Polaron e5100, by Quorum Technologies (1 mentions)
Jsm 7500f, by JEOL (1 mentions)
Quantax 200, by Bruker (1 mentions)
Quanta 200 feg esem, by Thermo Fisher Scientific (1 mentions)
Szx16, by Olympus (1 mentions)
Su8230, by Hitachi (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
23 protocols using quorum q150t es
1
Scanning Electron Microscopy of Plant Primordia
2
Nanoparticle Morphology Analysis by FE-SEM
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Nanoparticle morphology was determined by a field emission-scanning electron microscope (FE -SEM Zeiss Sigma 300, Zeiss, Germany). SEM sample stage was prepared by placing a double-sided adhesive carbon tape on an aluminum stub. One small drop of 1 mg/ml nanoparticle sample suspended in ultrapure water was placed on the sample stage and then dried at 37°C overnight. Subsequently, the dried sample was sputtered under vacuum with a chromium layer of approximately 100 Å thickness (Quorum Q150T ES, Quorum Technologies, UK) prior to analysis.
3
Fiber Optic-SPR Probe Preparation
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A benchtop FO-SPR biosensor, introduced by our group and commercialized by FOx Biosystems (Diepenbeek, Belgium), was used to perform FO-SPR bioassays for EV detection. FO-SPR probes were prepared manually for each experiment as previously described [40 (link),41 (link)]. In summary, a final length of 4.3 cm of FO-SPR probes was cut from a multimode optical fiber (TEQS, Thorlabs, Munich, Germany) with a core diameter of 400 μm and endings were stripped and cleaned, leaving 0.6 cm on the sensor side. Later, a thin layer (~50 nm) of gold was sputtered using a sputter coater (Quorum Q150T ES, Quorum Technologies, East Sussex, UK). Gold-coated FO-SPR probes were functionalized at 4 °C in a 0.1 mM ethanol/COOH SAM solution (volume ratio of 9:1) for 2 days. Finally, just before the experiment, the probes were washed with ethanol to remove any unbound material and used immediately.
4
Platelet Ultrastructure Imaging via SEM
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Gel-filtered platelets were fixed in 2% glutaraldehyde in 50 mM sodium cacodylate buffer (pH 7.5), containing 150 mM NaCl, for 90 min at room temperature. The fixed platelets were layered on a carbon filter (0.1 or 0.4 μm pore size) and centrifuged at 150× g for 5 min. The samples were rinsed three times with the cacodylate buffer for 5 min, dehydrated in ascending concentrations of ethanol, immersed in hexamethyldisilazane, and dried overnight. A thin film of gold palladium was layered on the samples using a sputter coater Quorum Q 150T ES (Quorum, Lewes, UK). Micrographs were taken with a scanning electron microscope (Merlin, Carl Zeiss, Jena, Germany).
5
Silver Nanoparticle Deposition on Titanium Nitride
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For the deposition of AgNPs a sputter coater (Quorum Q150T ES, Quorum Technologies, Laughton, UK) equipped with a silver target (70-AG5710 Silver Target 99.97% pure, Micro to Nano V.O.F., Haarlem, Netherlands) were used to prepare a set of samples with different AgNPs loadings, which depends on the sputtering time in the range of 10–60 s at 50 mA. The samples will be hereinafter referred to as x target_AgNPs/TNT, where x stands for the time of sputtering.
Table 1 presents the summary of the naming of the performed electrodes.
6
SEM Imaging of Tensile and Tear Specimens
7
Optimized Electron Microscopy Leaf Analysis
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An optimized and adapted method [68 ] was used for electron microscopy analyses. Shortly after harvest, the leaves were placed in 2.7% glutaraldehyde (GT) solution in 0.1 M PBS (phosphate buffer saline) at pH 7.2. After 1 h, GT was changed and left for an additional hour, after which the samples were thoroughly washed four times with PBS. For SEM, the leaves were cut in half and further dehydrated using increasing concentrations of acetone as follows: 30 min at 30%, 30 min at 50%, 30 min at 70%, 1 h at 80%, 1 h at 90%, 4 h at 100%, changing the solution every 1 h. Subsequently, the samples were placed in hexamethyldisilazane:acetone solution (1:2, 1:1, 2:1, 1:0 for 1 h each). All steps were conducted at 4 °C and the reagents were acquired from Sigma Aldrich (Merck, Bucharest, Romania). For examination, SEM HITACHI SU8230 (Hitachi, Tokyo, Japan) was used at an acceleration voltage of 30 kV after the samples were covered with a 9-nm- thick layer of gold, using the Quorum Q150T ES turbomolecular pumped coater (Quorum Technologies, London, UK). Each parameter was measured three times and form one leaf. At least six independent measurements were taken in six different spots on the leaf.
8
Structural Analysis of Polyelectrolyte Films
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Fourier transform
infrared (FT-IR) spectroscopy (Spectrum Two, PerkinElmer) was used
to examine pure PEs (PAA, PAA with ammonia, PEI) and the mixed films.
Freestanding pieces of PE films were prepared on a hydrophobic Teflon
plate, followed by drying in the fume hood. To remove water, all samples
were dried under vacuum at 30 °C for 24 h. The measurements were
conducted in the reflectance mode at a spectral resolution of 4 cm–1 from wavenumber 400 to 4000 cm–1. For each measurement, a minimum of 16 scans was conducted.
Film morphology and defects were examined by a scanning electron
microscope (SEM, JSM-6010LA, JEOL, Japan). All samples were stored
under vacuum at 30 °C for 24 h before imaging. To induce conductivity,
a Pt/Pd 5 nm coating was sputtered on the samples (Quorum Q150T ES,
Quorum Technologies, Ltd., U.K.).
To show the film responses
to water, dried freestanding pieces
of PE films were put into deionized water and stirred for 5 min with
a stirring bar. A 3:1 PEI/PAA oxygen permeation test sample was put
into water and then ammonia to demonstrate the possibility of recycling.
infrared (FT-IR) spectroscopy (Spectrum Two, PerkinElmer) was used
to examine pure PEs (PAA, PAA with ammonia, PEI) and the mixed films.
Freestanding pieces of PE films were prepared on a hydrophobic Teflon
plate, followed by drying in the fume hood. To remove water, all samples
were dried under vacuum at 30 °C for 24 h. The measurements were
conducted in the reflectance mode at a spectral resolution of 4 cm–1 from wavenumber 400 to 4000 cm–1. For each measurement, a minimum of 16 scans was conducted.
Film morphology and defects were examined by a scanning electron
microscope (SEM, JSM-6010LA, JEOL, Japan). All samples were stored
under vacuum at 30 °C for 24 h before imaging. To induce conductivity,
a Pt/Pd 5 nm coating was sputtered on the samples (Quorum Q150T ES,
Quorum Technologies, Ltd., U.K.).
To show the film responses
to water, dried freestanding pieces
of PE films were put into deionized water and stirred for 5 min with
a stirring bar. A 3:1 PEI/PAA oxygen permeation test sample was put
into water and then ammonia to demonstrate the possibility of recycling.
9
Bacterial Colony Preparation for SEM
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Prior to imaging, bacterial colonies were overlayered with 2.5% glutaraldehyde and 1% paraformaldehyde in 0.1 M phosphate buffer, pH = 7.4. After fixation, the bacterial colonies were cut out and the bulk of the supporting agar was carefully trimmed away prior to washing in ddH2O and stepwise ethanol dehydration. The colonies were finally subjected to critical‐point‐drying (Leica EM CPD 030, Wetzlar, Germany), mounted on specimen stubs using carbon adhesive tabs and sputter coated with platinum (Quorum Q150T ES). SEM images were acquired using an Ultra 55 field emission scanning electron microscope (Zeiss, Oberkochen, Germany) at 5kV and the SE2 detector.
10
Scanning Electron Microscopy Preparation
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