The human pancreatic ductal adenocarcinoma cell lines BxPC-3 (EP-CL-0042, Elabscience, Houston, TX, USA) and PANC-1 (CRL-1469, ATCC, Manassas, VA, USA), and primary human normal dermal fibroblasts (hNDF) (C-12302, Promocell, Heidelberg, Germany), were cultured at 10,000 cells/cm2 for no more than 15 passages in DMEM (DMEM-HXA, Capricorn, Ebsdorfergrund, Germany) or RPMI (RPMI-XA, Capricorn) in the case of BxPC-3, supplemented with 10% Fetal Bovine Serum (FBS) (S1810; Biowest, Nuaillé, France), L-glutamine (X055, Biowest) and Penicillin/Streptomycin (P/S) (L0022, Biowest). Cultures were maintained at 37 °C and 5% CO2 in a humidified atmosphere.
Rpmi xa
Manufactured by Capricorn
Sourced in Germany
RPMI-XA is a cell culture medium designed to support the growth and maintenance of a variety of cell types. It provides the necessary nutrients, vitamins, and growth factors required for optimal cell proliferation and survival in laboratory settings.
Automatically generated - may contain errors
2 protocols using rpmi xa
1
Culturing Pancreatic and Dermal Cells
2
Cell Culture Protocols for Pancreatic and Breast Cancer Lines
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ANC-1 (CRL-1469, ATCC, Manassas, VA, USA), MiaPaCa-2 (85062806, ECACC, Porton Down, UK), BxPC-3 (EP-CL-0041, Elabscience, Houston, TX, USA), MCF-7 (EP-CL-0149, Elabscience) and hNDF (C-12302, Promocell, Heidelberg, Germany) were cultured at 10,000 cells/cm2 for no more than 15 passages in DMEM (DMEM-HXA, Capricorn, Ebsdorfergrund, Germany) or RPMI (RPMI-XA, Capricorn) (in the case of BxPC-3 cells) supplemented with 10% FBS (S1810, Biowest, Nuaillé, France), L-glutamine (X055, Biowest) and Penicillin/Streptomycin (L0022, Biowest). Cultures were maintained at 37 °C and 5% CO2 in a humidified atmosphere.
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