Log In Sign up
The largest database of trusted experimental protocols

Anti p70s6k 9202

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

Anti-p70S6K #9202 is a primary antibody that recognizes the p70 S6 kinase (p70S6K) protein. p70S6K is a serine/threonine protein kinase that plays a key role in the regulation of cell growth and proliferation.

Automatically generated - may contain errors

Lab products found in correlation

Anti erk sc94, by Santa Cruz Biotechnology (3 mentions) Anti p70s6k thr389 9206, by Cell Signaling Technology (3 mentions) Anti erk tyr204 sc7383, by Santa Cruz Biotechnology (3 mentions) Anti kras sc30, by Santa Cruz Biotechnology (2 mentions) Anti s6 ser235 236 ribosomal protein 2211, by Cell Signaling Technology (2 mentions) Anti s6 ribosomal protein 2217, by Cell Signaling Technology (2 mentions) Anti 4e bp1 thr37 46 2855, by Cell Signaling Technology (2 mentions) Anti 4e bp1 9644, by Cell Signaling Technology (2 mentions) Anti 4e bp1 thr37 46, by Cell Signaling Technology (1 mentions) Nuclear extraction kit, by Abcam (1 mentions) Anti akt 9272, by Cell Signaling Technology (1 mentions) Anti actin sc 47778, by Santa Cruz Biotechnology (1 mentions) Anti lamin b sc 374015, by Santa Cruz Biotechnology (1 mentions) Anti pakt ser473 4060, by Cell Signaling Technology (1 mentions) Anti fatty acid synthase fasn, by Cell Signaling Technology (1 mentions) Anti 4e bp1, by Cell Signaling Technology (1 mentions) Anti β actin a5316, by Merck Group (1 mentions) Anti phospho ulk1 ser757, by Cell Signaling Technology (1 mentions) Anti ulk1, by Cell Signaling Technology (1 mentions) Anti α tubulin t6199, by Merck Group (1 mentions)

4 protocols using anti p70s6k 9202

1

Immunoblotting Analysis of Signaling Pathways

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were extracted and visualized as reported in14 (link). Immunoblotting was carried out with the following antibodies: anti-p70S6K(Thr389) #9206, anti-p70S6K #9202, anti-S6(Ser235/236) ribosomal protein #2211, anti-S6 ribosomal protein 2217#, anti-4E-BP1(Thr37/46) #2855, anti-4E-BP1 #9644 provided by Cell Signalling Technology. Anti-Erk #sc94, anti-Erk(Tyr204) #sc7383, anti-KRAS #sc30 were obtained from Santa Cruz Biotechnology.
Caiola E., Frapolli R., Tomanelli M., Valerio R., Iezzi A., Garassino M.C., Broggini M, & Marabese M. (2018). Wee1 inhibitor MK1775 sensitizes KRAS mutated NSCLC cells to sorafenib. Scientific Reports, 8, 948.
+ Open protocol
+ Expand
2

Protein Extraction and Immunoblotting Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were extracted and visualized as reported in [34 (link)]. Separation of nuclear protein extracts from cytoplasmic proteins was performed by using a nuclear extraction kit (AbCam, Cambridge, UK) following manufacturer’s instructions. Immunoblotting was carried out with the following antibodies: anti-p70S6K(Thr389) #9206, anti-p70S6K #9202, anti-pAkt (Ser473) #4060, anti-Akt #9272, anti-S6(Ser235/236) ribosomal protein #2211, anti-S6 ribosomal protein 2217#, anti-4E-BP1(Thr37/46) #2855, anti-4E-BP1 #9644, anti-Acetyl-CoA carboxylase (ACC) #3662, anti- Stearoyl-CoA desaturase 1 (SCD1) #2794, and anti-Fatty Acid Synthase (FASN) #3189, provided by Cell Signalling Technology (Danvers, MA, USA). Anti-Erk #sc94, anti-Erk (Tyr204) #sc7383, anti-SREBP1 #sc-365513, anti-actin #sc-47778, and anti-lamin B #sc-374015 were obtained from Santa Cruz Biotechnology (Dallas, TX, USA).
Colombo M., Passarelli F., Corsetto P.A., Rizzo A.M., Marabese M., De Simone G., Pastorelli R., Broggini M., Brunelli L, & Caiola E. (2022). NSCLC Cells Resistance to PI3K/mTOR Inhibitors Is Mediated by Delta-6 Fatty Acid Desaturase (FADS2). Cells, 11(23), 3719.
+ Open protocol
+ Expand
3

Protein Extraction and Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were extracted and visualized as reported in [30 (link)]. Immunoblotting was carried out with the following antibodies: anti-p70S6K(Thr389) #9206, anti-p70S6K #9202, anti-S6(Ser235/236) ribosomal protein #2211, anti-S6 ribosomal protein 2217#, anti-4E-BP1(Thr37/46) #2855, anti-4E-BP1 #9644 provided by Cell Signalling Technology. Anti-Erk #sc94, anti-Erk(Tyr204) #sc7383, anti-KRAS #sc30 and anti-tubulin#9104 were obtained from Santa Cruz Biotechnology. Anti-LC3 #PM036 was obtained from MBL.
Caiola E., Brunelli L., Marabese M., Broggini M., Lupi M, & Pastorelli R. (2016). Different metabolic responses to PI3K inhibition in NSCLC cells harboring wild-type and G12C mutant KRAS. Oncotarget, 7(32), 51462-51472.
+ Open protocol
+ Expand
4

Antibody Verification for Autophagy

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA): anti-LC3B #4108, anti-p70S6K #9202, anti-phospho-p70S6K (Thr389), #9205, anti-phospho-ULK1 (Ser 757) (#14202) and anti-ULK1 (#8054). From Sigma-Aldrich, anti-β-actin (A5316) and anti-α-tubulin (T6199) were used.
Palomino O., García-Aguilar A., González A., Guillén C., Benito M, & Goya L. (2021). Biological Actions and Molecular Mechanisms of Sambucus nigra L. in Neurodegeneration: A Cell Culture Approach. Molecules, 26(16), 4829.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!