Analytical gas chromatography was performed at a HP 6890 Series GC (Agilent, stationary phase: HP-5 column, poly-dimethyl/diphenyl-siloxane, 95/5) with a flame ionization detector using the following temperature profile: 60 °C (hold 3 min), then 15 °C/min to 250 °C (hold 5 min). The amounts of mesitylene and sodorifen were determined by peak area integration. Mass spectrometric analysis was performed with electron impact ionization (EI, 70 eV) on a Agilent HP 6890 Series GC–MS (Agilent, stationary phase: HP-5MS column, poly-dimethylsiloxane, 30 m, mass detection: Agilent 5973 Network Mass Selective Detector) using the same temperature profile as for analytical GC. NMR spectra of sodorifen and the E. coli-derived indole were directly recorded from head-space samples on a Bruker AVHD500 and a Bruker AV500-cryo spectrometer in CDCl3 (see Additional file 1 : Figure S6).
Hp 6890 series gc
Manufactured by Agilent Technologies
Sourced in United States, Germany
The HP 6890 series GC is a gas chromatograph designed for analytical separation and detection of chemical compounds. It features automated sample injection, temperature programming, and various detector options for specific analytical needs. The HP 6890 series GC provides reliable and precise separation and identification of complex mixtures in a variety of industries and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Agilent 5973 network mass selective detector, by Agilent Technologies (1 mentions)
Avhd500, by Bruker (1 mentions)
Hp ultra 2 capillary column, by Agilent Technologies (1 mentions)
Varian 9012 solvent delivery system, by Agilent Technologies (1 mentions)
Hp 5973 msd, by Agilent Technologies (1 mentions)
Nh2 semi prep column, by Waters Corporation (1 mentions)
Agilent 6890 series, by Agilent Technologies (1 mentions)
5973 mass selective detector, by Hewlett-Packard (1 mentions)
Zb 5ht capillary column, by Phenomenex (1 mentions)
Hp 5ms, by Agilent Technologies (1 mentions)
Agilent 5973 ms, by Agilent Technologies (1 mentions)
Dvb car pdms fiber, by Merck Group (1 mentions)
Spb 624 capillary column, by Merck Group (1 mentions)
8 protocols using hp 6890 series gc
1
Analytical GC-MS Characterization of Volatile Compounds
2
Quantifying Higher Alcohols and Esters in Beer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Higher alcohols and esters were quantified according to MEBAK WBBM 2.21.1 [27 ] as volatile fermentation byproducts with a gas chromatography flame ionization technique (GC-FID). The system comprised an HP 7694 Headspace Sampler and an HP 6890 Series GC from Agilent (Waldbronn, Germany). An HP-Ultra 2 capillary column (50 m × 0.32 mm × 0.52 µm) was used for chromatographic separation from Agilent. Samples were used directly without any treatment. Then, 5 mL beer and 0.1 mL of the internal standard (100 mg/L methyl caproate and 2 g/L butanol in 10 v/v% ethanol in water) were transferred in a 22 mL headspace vial and directly analyzed.
3
NPLC-UV and GC/MS Analysis of Compounds
4
Quantitative Analysis of Deuterated Citrate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Deuterated citrate (d4Citrate) was added to each sample to a final concentration of 0.1 mM as an internal standard. Metabolites were then extracted using cold methanol before being dried under vacuum desiccation. The samples were re-suspended in anhydrous pyridine containing the derivitisation agents methoxyamine hydrochloride followed by N-Methyl-N-trimethylsilyltrifluoroacetamide with 1% 2,2,2-Trifluoro-N-methyl-N-(trimethylsilyl)-acetamide, Chlorotrimethylsilane (MSTFA + 1%TMCS). GCMS was performed in pulsed splitless mode on a Hewlett Packard HP6890 series GC system with Agilent 6890 series injector and a 30 m long 250 µm diameter capillary column (Agilent, model number 19091s-433HP5MS) using a flow rate of 1 mL/minute, and a Hewlett Packard 5973 Mass selective detector. The acquisition was conducted in selective ion monitoring mode, the ion masses detected for citrate were: 273, 347, 375 and 465 and the corresponding heavy ions were 276, 350, 378 and 469. The dwell time for all of these ions was 50 ms.
5
Fatty Acid Methyl Ester Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fatty acid methyl ester analysis was implemented based on the method described by Moumen et al. [8 (link)]. Fatty acid methyl esters (FAMES) were obtained by transesterification of 10 mg of CPSO in boron trifluoride (10% in methanol) by heating at 70℃ for 90 min.
FAMES obtained were identified using a HP6890 Series–GC (Germany) coupled to a flame ionization detector (FID).
Prepared samples (0.5 µL) were injected in a 30 m × 250 µm × 0.25 µm Factor Four VF-Wax MS capillary column (Agilent P/N: CP9205) using an automatic injection system. The obtained signals were integrated using an Agilent ChemStation GC system. A comparison of obtained peaks was made with an authentic FAMES standard solution (10 mg/mL) that was run on the same column with the same conditions. The quantification of identified FAMES was computed automatically by a software signal integrator.
FAMES obtained were identified using a HP6890 Series–GC (Germany) coupled to a flame ionization detector (FID).
Prepared samples (0.5 µL) were injected in a 30 m × 250 µm × 0.25 µm Factor Four VF-Wax MS capillary column (Agilent P/N: CP9205) using an automatic injection system. The obtained signals were integrated using an Agilent ChemStation GC system. A comparison of obtained peaks was made with an authentic FAMES standard solution (10 mg/mL) that was run on the same column with the same conditions. The quantification of identified FAMES was computed automatically by a software signal integrator.
6
GC-MS Analysis of Analytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The analyses were performed using a Hewlett Packard gas chromatograph (HP 6890 series GC) with a 5973-mass detector (Agilent, Santa Clara, USA). The analytes were separated using ZB-5HT capillary column (5% diphenyl- and 95% dimethylpolysiloxane stationary phase, 30 m of length, an inner diameter of 0.32 mm, and a film thickness of 0.25 μm; Phenomenex Inc., Torrance, CA, USA). The carrier gas was helium with a constant flow of 2 ml/min. The oven was programmed as follows: initial 40 °C, kept for 5 min, then gradually increased by 3 °C/min to 180 °C, then changed by 15 °C min to 280 °C, and finally held for 1 min. The injector was set to 250 °C in spitless mode. After five minutes the injector was vented.
The GC–MS interface temperature was set to a temperature of 300 °C, the MS ion source temperature was 230 °C, and the scan range was 30–550 m/z.
The GC–MS interface temperature was set to a temperature of 300 °C, the MS ion source temperature was 230 °C, and the scan range was 30–550 m/z.
7
Cholesterol Content in Egg Yolk
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cholesterol content of egg yolk was measured using the method described by Vanderplanck et al. [28 (link)]. After saponification with KOH and extraction with diethyl ether, cholesterol was separated from the fat. The mixture was analyzed by gas chromatography using a Hewlett-Packard chromatograph (HP 6890 series GC) equipped with a capillary column (HP 5 MS (5 %-phenylmethylpolysiloxane, 30m × 0:25mm, 0.25 m film thickness), Agilent Technologies, Palo Alto, CA, USA) and a flame ionization detector. The injector was operated in splitless mode. The operating parameters were as follows: carrier gas: helium at 1 mL min−1; column temperature: 275°C; injector and detector temperatures: 250 and 300°C, respectively; and injection volume: 1 μL. The cholesterol concentration in egg yolk was calculated and expressed as mg per g of egg yolk.
8
Oxidation Products in Drinkable Yogurts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The secondary oxidation products of plain and functionalized drinkable yogurts (HFY0, HFY0.5, HFY1, LFY0, LFY0.5, and LFY1) that contain cod liver oil without α-tocopheryl acetate and tocopheryl extract were extracted by solid phase microextraction (SPME) and analyzed with gas chromatography coupled with mass spectrometry (GC-MS), using the same method described by Lehtonen et al. [24] . Three replicates consisting of approximately 2 g of sample that had been weighed in amber glass vials, hermetically sealed, and stored at 4 °C in the dark were withdrawn for analysis. Briefly, the volatile compounds were adsorbed onto a DVB/CAR/PDMS fiber (Supelco, USA), released in the GC-MS injector at 250 °C for 10 min, and analyzed using an HP 6890 series GC coupled with an Agilent 5973 MS (Agilent Technologies Inc., USA). They were separated using a SPB-624 capillary column (Supelco, USA) and a temperature program from 40 to 200 °C with helium at a flow rate of 0.7 mL/min with the MS running in full-scan mode. The compounds were identified based on the comparison of their retention times and mass spectra with those of the standards.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!