All studies involving animals were performed under a protocol (A 6-13-13) approved by the Institutional Animal Care and Use Committee of Wayne State University. Sprague-Dawley rats (200–250 g, N = 3) were anesthetized with 3% isoflurane in oxygen and maintained at 2% isoflurane in oxygen throughout the imaging studies. The body temperature was maintained using electronically-controlled heating pad (M2M Imaging, Cleveland, OH) set at 37°C. Anesthetized rats were placed in the microPET R4 scanner (Siemens, Knoxville, TN) in the supine position with the long axis of the animal parallel to the long axis of the scanner with the brain positioned in the center of the field of view. Each radiotracer (300–500 μCi/animal) was administered in saline via the tail-vein injection in a total volume ≤1.25 ml. Dynamic PET images were obtained over 60 minutes, followed by 2 overlapping frames (5 min each) acquired to obtain a whole body images of radiotracer biodistribution in other organs and tissues. After PET imaging, the positioning bed with the affixed anesthetized animal was transferred to the Inveon SPECT/CT scanner (Siemens, Knoxville, TN) and CT images and 4 overlapping frames (2 min each) were acquired covering the whole body using X-ray tube settings of 80 kV and 500 uA.
Inveon spect ct scanner
Manufactured by Siemens
Sourced in United States
The Inveon SPECT/CT scanner is a preclinical imaging system designed for small animal research. It combines single-photon emission computed tomography (SPECT) and computed tomography (CT) technologies to provide high-resolution, multimodal imaging capabilities. The system is designed to acquire anatomical and functional data simultaneously, enabling researchers to study disease progression, evaluate therapeutic interventions, and conduct a wide range of preclinical studies.
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6 protocols using inveon spect ct scanner
1
Multimodal Imaging of Rat Biodistribution
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Multimodal Imaging of Sprague-Dawley Rats
3
Radiosynthesis and Administration of [18F]TFAHA
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The radiosynthesis and formulation of [18F]TFAHA for intravenous (i.v.) injection was performed as previously described42 (link). Under inhalation anesthesia (as described above), the rat head fixed in a stereotactic holder made of polycarbonate plastic (Kopf-Tujunga, Germany), attached to the bed of microPET R4 scanner (Siemens, Knoxville, TN), and the head was positioned in the center of the field of view (FOV). Then, the rat was injected via the tail vein with [18F]TFAHA (350–500 µCi in 1 ml), as a steady injection over 1 min; PET images were obtained in a dynamic mode over 30 minutes post radiotracer administration. Thereafter, the detachable bed with the affixed animal was transferred into the Inveon SPECT/CT scanner (Siemens, Knoxville, TN) and CT images of the head were acquired using 80 kV and 500 uA current settings with exposure time of 300–350 milliseconds at each of the 360 rotational steps.
4
Multimodal Imaging of Brain PET/CT
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Baseline of 2-[18F]BzAHA PET/CT studies was performed a day after the MRI studies. The radiosynthesis and formulation of 2-[18F]BzAHA for intravenous (i.v.) injection was performed as previously described45 (link); under inhalation anesthesia (as described above). Anesthetized rats were placed in a stereotactic head holder made of polycarbonate plastic (Kopf-Tujunga) and attached to the bed the microPET R4 scanner (Siemens) in the supine position with the long axis of the animal parallel to the long axis of the scanner and the brain positioned in the center of the FOV. The radiotracer (300–500 µCi/animal) was administered in saline via the tail vein in a total volume of ≤1.25 mL, as a slow bolus injection over the period of 1 min. Dynamic PET images were obtained over 60 min. After PET imaging, the positioning bed with the affixed anesthetized animal was transferred to the Inveon SPECT/CT scanner (Siemens) and CT images were acquired in 4 overlapping frames (2 min each) covering the whole body using X-ray tube settings of 80 kV and 500 µA with exposure time of 300–350 ms of each of the 360 rotational steps.
5
In Vivo PET/CT Imaging of Radiolabeled Peptides
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For imaging studies, [64Cu]1 and [64Cu]2 (7.77–8.88 MBq) in PBS (100 μL, pH 7.2) were injected i.v. via a catheter into the tail vein of mice (n = 3/radiolabeled peptide) anesthetized with 2–3% isoflurane in medical grade oxygen. Animals were imaged in a prone position two at a time side by side. PET/CT scans were acquired using Inveon scanners (Inveon DPET scanner and Inveon SPECT/CT scanner, Siemens Medical Solutions, Knoxville, TN, USA; PET scans: a static 15 min scan at 4 h p.i., static 30 min scans at 24 and 48 h p.i., and a static 1 h scan at 72 h p.i.) and analyzed as previously described using the Inveon Research Workplace software (Siemens) [42 (link),44 (link)].
6
Radiotracer-Based Tumor Imaging Protocol
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These studies were performed 15 d after inoculation. Under isoflurane/air anesthesia, saline containing 123 I-IIMU (667 kBq/0.1 mL) was injected through the tail vein. At 30 min after injection, tumor and control tissues were collected and weighed, and their radioactivity was measured using a single-channel g-counter (Ohyo Koken Kogyo). Radioactivity was expressed as a percentage injected dose per gram of tissue.
SPECT/CT SPECT/CT imaging was performed using an Inveon SPECT/CT scanner (Siemens Medical Solutions) with a double-head detector. Each head contained a 68 • 68 pixelated scintillator array. Each pinhole collimator had an aperture of 2.0 mm. The radius of rotation was 35 mm. Studies were performed 12 d after inoculation. A saline solution of 123 I-IIMU (25 MBq/0.1 mL) was injected through the tail vein under isoflurane anesthesia. At 45 min after administration, data were acquired for 30 min.
SPECT/CT SPECT/CT imaging was performed using an Inveon SPECT/CT scanner (Siemens Medical Solutions) with a double-head detector. Each head contained a 68 • 68 pixelated scintillator array. Each pinhole collimator had an aperture of 2.0 mm. The radius of rotation was 35 mm. Studies were performed 12 d after inoculation. A saline solution of 123 I-IIMU (25 MBq/0.1 mL) was injected through the tail vein under isoflurane anesthesia. At 45 min after administration, data were acquired for 30 min.
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