Thiol-modified DNA oligonucleotides (Integrated DNA Technologies) were treated with tris(2-carboxyethyl)phosphine (Sigma-Aldrich) for 30 minutes (50 nmole DNA per reaction) and immediately reacted with 1 µmole of 18:1 MPB PE (Avanti Polar Lipids) in aqueous solutions containing ~2% Octyl β-D-glucopyranoside (OG, purchased from EMD Millipore) at room temperature for 30 minutes. Such a reaction mixture was then diluted ~3-fold with OG concentration brought up to 4%, added to 2.5 µmole of pre-dried DOPC, agitated, and dialyzed overnight. The conjugation products were separated from unconjugated DNA via isopycnic centrifugation in iodixanol gradients (Cosmo Bio USA) and later analyzed by SDS-polyacrylamide gel electrophoresis.
Octyl β d glucopyranoside og
Manufactured by Merck Group
Sourced in United States
Octyl β-D-glucopyranoside (OG) is a non-ionic surfactant used in biochemical applications. It is a mild detergent derived from the sugar glucose and an octyl alcohol chain. OG is commonly used in the solubilization and purification of membrane proteins and in the preparation of liposomes and other lipid-based systems.
Automatically generated - may contain errors
Lab products found in correlation
His60 ni superflow resin, by Takara Bio (2 mentions)
Tris 2 carboxyethyl phosphine tcep, by Thermo Fisher Scientific (2 mentions)
Isopropyl β d thiogalactoside iptg, by Americanbio (2 mentions)
1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions)
1 2 dioleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (2 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (2 mentions)
Dioleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (2 mentions)
Dioleoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (2 mentions)
L α phosphatidylinositol 4 5 bisphosphate, by Avanti Polar Lipids (2 mentions)
N dodecyl β d maltopyranoside ddm, by Avanti Polar Lipids (2 mentions)
Tris 2 carboxyethyl phosphine, by Merck Group (1 mentions)
Thiol modified dna oligonucleotides, by Integrated DNA Technologies (1 mentions)
Mpb pe, by Avanti Polar Lipids (1 mentions)
Iodixanol gradients, by Cosmo Bio (1 mentions)
3 protocols using octyl β d glucopyranoside og
1
Lipid-Oligonucleotide Conjugation Protocol
2
Purification and Reconstitution of Membrane Proteins
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Chemicals were from the following sources: Isopropyl-β-D-thiogalactoside (IPTG) (AmericanBio), His60 Ni Superflow Resin (Takara), tris (2-carboxyethyl) phosphine (TCEP) (Thermo), Iodixanol solution (60%, w/v) (Cosmo Bio USA), Octyl β-D-glucopyranoside (OG) (EMD Millipore), n-dodecyl-β-D-maltopyranoside (DDM) (Avanti Polar Lipids). All DNA oligonucleotides were purchased from Integrated DNA Technologies. All lipids were obtained from Avanti Polar Lipids: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC); 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS); L-α-phosphatidylinositol- 4,5-bisphosphate [PI(4,5)P2]; 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2–1,3-benzoxadiazol-4-yl) (NBD-PE); 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2–1,3-benzoxadiazol-4-yl) (NBD-PS); 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod-PE); 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid) succinyl] [DGS-NTA(Ni)]; 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[4-(p-maleimidophenyl)butyramide] (MPB-PE).
3
Purification and Reconstitution of Membrane Proteins
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