Dh351 2

The cells were fixed with 4% paraformaldehyde (Servicebio) for 10 min, permeabilized with 0.2% Triton X-100 (DH351-2; Beijing Dingguochangsheng Biotechnology, Co., Ltd., Beijing, China) for 20 min, and blocked with 1% bovine serum albumin (FA016; Beijing Dingguochangsheng Biotechnology, Co., Ltd.) for 30 min. Then, the fixed cells were incubated with diluted primary antibodies overnight at 4 °C. The primary antibodies used in this study are listed in Supplementary Table 1. Then the cells were incubated with the appropriate secondary antibody (1:1000 dilution; A21202; Invitrogen) for 1 h at 37 °C and protected from light. Hoechst 33342 (H1399; Invitrogen) was used to stain the cell nuclei for 10 min, and fluorescence intensity was detected and analyzed using the Cellomics ArrayScanVTI HCS Reader (ThermoFisher Scientific, Rockford, IL, USA). Target protein expression levels are expressed as the mean average fluorescence intensity.