Proteins containing the V5 epitope were detected with a mouse monoclonal anti-V5 (Sigma, V8012) and HA-tagged proteins with a rabbit anti-HA (Sigma H6908). The rabbit polyclonals against eIF3i and eIF3h were sourced from Proteintech Group (11287-1-AP) and Sigma (AY50491), respectively. Other anti-eIF3 subunit antibodies used were; anti-eIF3a, Cell Signaling (#2538); anti-eIF3b, Santa Cruz (sc16377); anti-eIF3c, Sigma (E6408). A rabbit polyclonal against an N-terminal sequence of c-Myc was from Abcam (ab11917). Subunit-specific rabbit polyclonals against individual CCT subunits and an antibody to RPL7a have been characterized elsewhere (Roobol and Carden, 1999 (link)). α-tubulin was detected with the TAT mouse monoclonal (Woods et al., 1989 (link)) which was a kind gift from Prof. Keith Gull (University of Oxford, UK). β-actin was detected with the mouse monoclonal AC-15 (Sigma). For western blot detection of pulldown proteins a protein A-HRP conjugate (Millipore) was used, otherwise anti-rabbit, -mouse or -goat-HRP was used as secondary.
Anti eif3a
Manufactured by Cell Signaling Technology
Sourced in Canada
Anti-eIF3a is a primary antibody that specifically recognizes the eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3) complex. eIF3a is a crucial component of the eIF3 complex, which plays a central role in the initiation of protein synthesis.
Automatically generated - may contain errors
2 protocols using anti eif3a
1
Antibody Detection for Protein Analysis
2
Localization of IAV Proteins and Stress Granules
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For immunofluorescence microscopy, A549 cells were seeded on glass coverslips and cultured overnight prior to IAV-CA/07 infection at an MOI of 1 or mock infection. At the indicated times postinfection, cells were fixed with 4% paraformaldehyde and permeabilized with cold methanol as described previously (50 (link)). Cells were stained with goat polyclonal antibody to influenza A virus (catalog number ab20841; Abcam Inc., Toronto, ON, Canada), mouse anti-G3BP1 (catalog number 611126; BD Biosciences), mouse anti-PABP (catalog number 10E10; Santa Cruz), rabbit anti-TIAR (catalog number 8509; Santa Cruz), and anti-eIF3A (catalog number 3411; Cell Signaling), followed by Alexa-coupled donkey secondary antibodies (Thermo Fisher Scientific) at a 1:1,000 dilution, along with 5 ng/ml Hoechst dye (blue). Images were captured using a Zeiss AxioImager Z2 microscope. Bars represent 20 μm.
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