Cells were seeded in 96-well plates at a density of 2 × 103 cells/well. After 0–72 h, a mixture of CyQUANT NF Cell Proliferation dye reagent and deliverer (Invitrogen, Waltham, MA, USA) was added and incubated at 37 °C for 30 min. Fluorescence intensity was measured as the ratio of the fluorescence at 530 nm to that at 485 nm using an infinite M200 Pro microplate reader (TECAN, Männedorf, Switzerland).
Cyquant nf cell proliferation dye reagent
Manufactured by Thermo Fisher Scientific
Sourced in United States
The CyQUANT NF Cell Proliferation dye reagent is a fluorescent dye that binds to cellular nucleic acids, allowing for the quantitative measurement of cell proliferation. It provides a simple and sensitive method for determining the relative number of cells in a sample.
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3 protocols using cyquant nf cell proliferation dye reagent
1
Cell Proliferation Assay Protocol
2
Cell Proliferation Assay Protocol
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Suspensions of 1.0 to 2.0 × 103 cells were seeded into 96-well. After 0–144 h of incubation at 37 °C, a mixture of CyQUANT NF Cell Proliferation dye reagent and deliverer (Invitrogen) was added. After 4 h of incubation, the ratio of the fluorescence intensity of 530 to that at 485 nm was measured.
3
Quantifying Cell Proliferation with CyQUANT Dye
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Cells were transfected with siRhoBTB3, siCol1a1, and/or Col1a1 plasmid DNA for 24 h, trypsinized, and resuspended in medium. After 48 h, the cells were seeded in 96-well plates at a density of 4 × 103 cells/well. After 72 h of treatment, a mixture of CyQUANT NF Cell proliferation dye reagent and deliverer (Invitrogen) was added to the wells, and the plates were incubated at 37°C for 30 min. Fluorescence intensity was measured as the ratio of fluorescence at 530 nm to that at 485 nm.
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