The PC cell lines BxPC-3 and MiaPaCa-2 were obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Ibaraki, Japan), cultured, and stored based on the suppliers’ instructions. The cells were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, 100 μg/mL streptomycin, 2 g/mL NaHCO3, 1 mM sodium pyruvate, and 0.1 mM NEAA. All procedures were performed as described previously [26 (link),27 (link),28 (link),29 (link)].
Bxpc 3
Manufactured by Japanese Collection of Research Bioresources Cell Bank
Sourced in Japan
BxPC-3 is a cell line derived from a human pancreatic adenocarcinoma. It is a commonly used tool in cancer research and drug discovery.
Automatically generated - may contain errors
Lab products found in correlation
Panc 1, by American Type Culture Collection (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Mia paca 2, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Time crl 4025, by American Type Culture Collection (1 mentions)
Kato 3, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Aspc 1, by American Type Culture Collection (1 mentions)
Hepg2, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Nci h460, by American Type Culture Collection (1 mentions)
Mcf 7, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Hep3b, by American Type Culture Collection (1 mentions)
Plc prf 5, by American Type Culture Collection (1 mentions)
Panc1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Smmc 7721, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Bel 7402, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Hepg2, by American Type Culture Collection (1 mentions)
Plc prf 5, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Hep3b, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Sk hep 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Tera 1, by American Type Culture Collection (1 mentions)
Bxpc 3, by American Type Culture Collection (1 mentions)
5 protocols using bxpc 3
1
Culturing and Maintaining PC Cell Lines
2
Comparative Analysis of Cancer Cell Lines
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The OVSAYO, OVISE, OVSAHO, MCAS, and MDA‐MB‐231 cell lines were as previously described.30 The AsPC‐1, PANC‐1, and TIME (CRL‐4025) cell lines were purchased from ATCC. The YMB‐1, KP2, KP3, BxPC‐3, and KATOIII cell lines were purchased from the Japanese Collection of Research Bioresources Cell Bank. Cell lines, except for TIME, were maintained in RPMI‐1640 medium with 10% FCS under normoxia and hypoxia, as previously described.31 TIME cells were cultured according to the manufacturer’s instructions.
3
Cell Line Culture Methodology
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HEK-293T, NCI-H1299, BxPC-3, PANC-1, Hep3B, PLC/PRF/5,HepG2, SK-HEP-1, MCF7, A549, NCI-H460, Tera-1 and Tera-2 were purchased from ATCC; HuH-7 was purchased from Japanese Collection of Research Bioresources (JCRB), SMMC-7721 and BEL-7402 were purchased from Typical culture preservation commission cell bank, Chinese academy of sciences (NCB); MHCC-97L and LM3 were gifts from Zhongshan Hospital, Fudan University (Shanghai, China); SMMC-7721, BEL-7402, MHCC-97L and LM3 used in this study have been described in previous publication [24] (link), [30] (link), [31] , [32] (link).
HEK-293T, NCI-H1299, BxPC3, PANC1, Hep3B, PLC/PRF/5, HepG2, HuH-7, HepG2, SK-HEP-1, SMMC-7721, 97L, LM3, BEL-7402 and MCF7 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum(FBS). A549 and NCI-H460 cells were cultured in RPMI1640 medium supplemented with 10% FBS. Tera1 and Tera2 cells were cultured in McCoy’s 5a medium supplemented with 15%FBS. All cell lines were cultured in the presence of antibiotics at 37°C with 5%CO2.
HEK-293T, NCI-H1299, BxPC3, PANC1, Hep3B, PLC/PRF/5, HepG2, HuH-7, HepG2, SK-HEP-1, SMMC-7721, 97L, LM3, BEL-7402 and MCF7 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum(FBS). A549 and NCI-H460 cells were cultured in RPMI1640 medium supplemented with 10% FBS. Tera1 and Tera2 cells were cultured in McCoy’s 5a medium supplemented with 15%FBS. All cell lines were cultured in the presence of antibiotics at 37°C with 5%CO2.
4
Establishing Primary CAFs from PC Tissues
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We established primary CAFs (CAF1 and CAF2) from surgically resected PC tissues at Gunma University using a previously described method.
16 (link) The resected PC samples were used in accordance with the Helsinki Declaration and Institutional Review Board of Gunma University (approval number: 2016‐118) after obtaining written informed consent. We used the CAFs between passage numbers 4 and 8. The human PC cell lines, Suit2 and BXPC3, were obtained from the JCRB Cell Bank and SW1990 was obtained from the ATCC. PANC1 and the human pancreatic stellate cell line hPSC5, derived from PC, was provided by RIKEN BRC. The human HSC line LX‐2 was purchased from Millipore. Cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin–streptomycin (Thermo Fisher Scientific) and maintained at 37°C in a humidified incubator with 5% CO2 atmosphere.
16 (link) The resected PC samples were used in accordance with the Helsinki Declaration and Institutional Review Board of Gunma University (approval number: 2016‐118) after obtaining written informed consent. We used the CAFs between passage numbers 4 and 8. The human PC cell lines, Suit2 and BXPC3, were obtained from the JCRB Cell Bank and SW1990 was obtained from the ATCC. PANC1 and the human pancreatic stellate cell line hPSC5, derived from PC, was provided by RIKEN BRC. The human HSC line LX‐2 was purchased from Millipore. Cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin–streptomycin (Thermo Fisher Scientific) and maintained at 37°C in a humidified incubator with 5% CO2 atmosphere.
5
Cultivation and Characterization of PDAC Cell Lines
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The human PDAC cell lines Capan-1, MIA PaCa-2, BxPC-3, and Panc-1 were obtained from the American Culture Type Collection. BxPC-3 cells stably transfected with the firefly Luc expression vector (BxPC-3-Luc) were obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan). Cells were cultured for no longer than 5 months following resuscitation. Authentication was not performed by the authors. MIA PaCa-2 and Panc-1 cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (FBS). BxPC-3 and BxPC-3-Luc cells were maintained in RPMI-1640 supplemented with 10% FBS. Capan-1 cells were maintained in Iscove’s modified Dulbecco’s medium supplemented with 20% FBS. All media were supplemented with 100 U/mL penicillin and 100 mg/mL streptomycin. Cells were maintained at 37°C in a humidified atmosphere with 5% CO2.
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