C9368

Manufactured by Merck Group

C9368 is a laboratory equipment product from Merck Group. It is designed for general use in scientific research and analysis applications. The core function of C9368 is to perform specific tasks required in a laboratory setting. No further details about its intended use or performance characteristics are provided.

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Lab products found in correlation

Lsm 880, by Zeiss (2 mentions) Alexa fluor 488 conjugated donkey antibody to chicken igg, by Jackson ImmunoResearch (1 mentions) Ht501128, by Merck Group (1 mentions) Vt1000, by Leica (1 mentions) D1306, by Thermo Fisher Scientific (1 mentions) Ab13970, by Abcam (1 mentions) Deconvolution microscope, by Zeiss (1 mentions) D5905, by Merck Group (1 mentions) L182 50, by Harvard Bioscience (1 mentions) E9884, by Merck Group (1 mentions) Dmrbe, by Leica (1 mentions) H 3401 500, by Vector Laboratories (1 mentions) C2404, by Merck Group (1 mentions) 3 3 diaminobenzidine (dab), by Merck Group (1 mentions)

3 protocols using c9368

Tissue Preparation and Immunostaining Protocol

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After each recording, probes were cleaned by soaking in undiluted trypsin solution (15400054, Gibco), rinsed in DI water and ethanol, and stored for possible reuse under ambient conditions. For histology, animals were perfused with ice cold phosphate buffered saline (PBS, pH 7.3, 10010023, Gibco) and formalin solution (neutral buffered 10 %, HT501128, Sigma) in sequence. After overnight fixation in formalin, the brain was sectioned into 100 μm sections on a vibratome (VT1000S, Leica). The section shown in figure 7B was stained with DAPI (D1306, Invitrogen). The sections shown in figures 8B, 8D, 9A were blocked using normal serum, then incubated overnight at 4 °C with chicken anti-GFP (ab13970, Abcam) as a primary antibody (1:1000 dilution). After washing three times with PBS, the sections were incubated at 4 °C with Alexa Fluor 488–conjugated donkey antibody to chicken IgG (703-545-155, Jackson ImmunoResearch) as a secondary antibody (1:200 dilution) for 4 hr. Finally, all sections were mounted using tissue mounting medium (C9368, Sigma), and imaged under laser confocal microscopy (LSM 880, Zeiss).

Yang L., Lee K., Villagracia J, & Masmanidis S.C. (2020). Open source silicon microprobes for high throughput neural recording. Journal of neural engineering, 17(1), 016036.

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Immunostaining of Fixed Tissue Samples

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Human frozen tissue arrays (FMC402d, FMC282d, FLV401a, BRF404b, FLU401a, FCO405a, AlenaBio) were fixed with precooled methanol for 5 min at − 20 °C. For immunostaining of H1299 and HepG2, cells were fixed in 4% PFA for 10 min. Fixed cells or cryostat sections were permeabilized in 0.2% Triton X-100 and blocked in 5% BSA for 1 h, and then incubated with DAPI, or the appropriate primary and secondary antibodies. Samples were mounted with mounting medium (C9368, Sigma). Images were acquired with a deconvolution microscope (Zeiss). Mitochondrial fragmentation count (MFC) was acquired as reported (Rehman et al., 2012 (link)).

Li T., Han J., Jia L., Hu X., Chen L, & Wang Y. (2019). PKM2 coordinates glycolysis with mitochondrial fusion and oxidative phosphorylation. Protein & Cell, 10(8), 583-594.

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Immunohistochemical Staining of Liver Tissue

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Liver tissue sections were dewaxed in xylene (9713.2; Carl Roth) and rehydrated in gradient ethanol (K928.4; Carl Roth). For antigen retrieval, slides incubated with 1 mM EDTA (E9884, pH 8.4; Sigma-Aldrich) or citrate acid buffer (C2404, pH 6.0; Sigma-Aldrich) were heated by microwave (15 s full power, 1000 W, 45 s shutdown, 10 cycles). Following cooling at room temperature for 30 min, the slides were incubated in blocking peroxide (S200389-2; Dako) for 30 min to reduce nonspecific staining. Subsequently, primary antibodies were added overnight at 4°C. The next day, following washing with PBS (L182-50; Biochrom) three times, sections were incubated with horseradish peroxidase-labeled secondary antibodies for 45 min. Visualization was achieved with 3,3-diaminobenzidine (D5905; Sigma-Aldrich). Counterstaining was performed with hematoxylin (H-3401-500; Vector Laboratories). Finally, all sections were dehydrated and mounted with malinol mounting medium (C9368; Sigma-Aldrich). Images were taken under a microscope (DMRBE; Leica).

Feng R., Kan K., Sticht C., Li Y., Wang S., Liu H., Shao C., Munker S., Niess H., Wang S., Meyer C., Liebe R., Ebert M.P., Dooley S., Ding H, & Weng H. (2022). A hierarchical regulatory network ensures stable albumin transcription under various pathophysiological conditions. Hepatology (Baltimore, Md.), 76(6).

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