Immunohistochemistry kit

Manufactured by Abcam

Sourced in United States

The Immunohistochemistry Kit is a laboratory equipment designed for the detection and visualization of specific target proteins within tissue sections. It provides the necessary reagents and protocols for the immunohistochemical staining process, allowing for the identification and localization of proteins of interest in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

9 protocols using immunohistochemistry kit

Immunohistochemical Analysis of Liver Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Liver tissues were fixed and paraffin embedding. Cryosections were prepared and used for immunofluorescence and immunohistochemistry.
Nonspecific antibody binding was blocked by goat serum. High-mobility group box 1 (HMGB1) and F4/80 staining was performed with anti-HMGB1 and anti-F4/80 antibody (Cell Signaling Technology, Danvers, MA) at 1:100 dilution in a humidified chamber at 4 °C overnight. After incubation with Alexa-conjugated secondary antibodies (Invitrogen) at 1:1000 dilution for 1 h at room temperature, signals were visualized by confocal microscopy, and DAPI was used for nuclear counterstaining. Quantification was using Image J software.
Human liver Sirt6 staining was performed using anti-Sirt6 antibody (Cell Signaling Technology) and immunohistochemistry kit (Abcam, Cambridge, UK) according to the manufacturer’s instructions. Images were created using CaseViewer software (3D Histech, Budapest, Hungary).
Mice liver PAR staining was performed using anti-PAR antibody (Tulips Biolabs, Lansdale, PA) and immunohistochemistry kit (Abcam) according to the manufacturer’s instructions. Quantification was using Image J software.

Luo J., Liu H., Xu Y., Yu N., Steiner R.A., Wu X., Si S, & Jin Z.G. (2024). Hepatic Sirt6 activation abrogates acute liver failure. Cell Death & Disease, 15(4), 283.

+ Open protocol

+ Expand

Colorectal Cancer Pathogenesis Study

Check if the same lab product or an alternative is used in the 5 most similar protocols

The chemicals, reagents, and antibodies are the following: phloretin and ruthenium (III) chloride hydrate, DPPH, penicillin, TPTZ, ABTS, CT-DNA (calf thymus DNA), foetal bovine serum (FBS), sodium pyruvate, MTT, Annexin V, propidium iodide (PI) (procured from Sigma-Aldrich Chemical Co.), DMH (dimethylhydrazine), streptomycin, DSS (dextran sodium sulfate), insulin, L-glutamine, streptavidin peroxidase, 3,3′-diaminobenzidine (DAB), and proteinase K. Antibodies specific for p53, PCNA, pro- and active caspase-3, Akt1, phospho Akt, mTOR, phospho mTOR, and VEGF (627501) were acquired from BioLegend (San Diego, CA, USA). Anti-rat antibody for Bcl-2, Bax, beta catenin, NF-κB, and goat anti-rabbit IgG secondary antibody were bought from Genetex, Inc. (Irvine, CA, USA). An immunohistochemistry kit was acquired from Biovision, Inc. (Milpitas, CA, USA), and an apoptosis detection kit was purchased from Takara Bio Inc. (Shiga, Japan).

Jin G., Zhao Z., Chakraborty T., Mandal A., Roy A., Roy S, & Guo Z. (2020). Decrypting the Molecular Mechanistic Pathways Delineating the Chemotherapeutic Potential of Ruthenium-Phloretin Complex in Colon Carcinoma Correlated with the Oxidative Status and Increased Apoptotic Events. Oxidative Medicine and Cellular Longevity, 2020, 7690845.

+ Open protocol

+ Expand

Diosmetin Mitigates Oxidative Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols

All reagents used for the experiment were of analytical grade. Diosmetin (purity>98%) was purchased from PureOne Biotechnology (Shanghai, China). 2,4,6-trinitrobenzene sulfonic acid (TNBS), biotinylated horseradish peroxidase, and 3,3 0 -diaminobenzidine (DAB) were purchased from Sigma Chemical, Co. (St. Louis, MO,USA). The assay test kits for the measurement of oxidative stress namely, superoxide dismutase (SOD), MPO, MDA were supplied by Multisciences (Lianke) Biotech, Co., Ltd. (Hangzhou, China). Anti-rat antibody for IL-6, NF-κB, TNF-α and goat anti-rabbit IgG secondary antibody were bought from Genetex, Inc. (Irvine, CA, USA). An immunohistochemistry kit was procured from Biovision, Inc. (Milpitas, CA, USA), and an apoptosis detection kit was purchased from Takara Bio Inc. (Shiga, Japan). Other reagents were purchased from local firms in their purest forms.

Yu X., & Wang W. (2021). Diosmetin attenuate experimental ulcerative colitis in rats via suppression of NF-κB, TNF-α and IL-6 signalling pathways correlated with down-regulation of apoptotic events. European Journal of Inflammation, 19, 205873922110672-205873922110672.

+ Open protocol

+ Expand

Immunohistochemical Analysis of AT1 Receptors

Check if the same lab product or an alternative is used in the 5 most similar protocols

AT₁ receptor immunohistochemistry of the arteries was performed with a commercially available immunohistochemistry kit (Abcam, MA, USA). Coronal sections (5 μm) were made from the isolated arteries and incubated overnight at 4°C with an antibody specific for the AT₁ receptor (diluted 1 : 500; Abcam, Cambridge, MA, USA) protein. The arteries were then incubated with a secondary antibody (biotinylated goat anti-rabbit IgG) for one hour, followed by staining with DAB in accordance with the manufacturer's instructions. AT₁ receptor immunoreactivity was observed using light microscopy (DP70, Olympus, Tokyo, Japan) after covering the section slides with mounting media.

Zhang F., Xu Y., Pan Y., Sun S., Chen A., Li P., Bao C., Wang J., Tang H, & Han Y. (2019). Effects of Angiotensin-(1-7) and Angiotensin II on Acetylcholine-Induced Vascular Relaxation in Spontaneously Hypertensive Rats. Oxidative Medicine and Cellular Longevity, 2019, 6512485.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Cancer Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemistry (IHC) staining was routinely carried out using an immunohistochemistry kit (abcam, UK). The tissue sections were deparaffinized and rehydrated. The antigen was retrieved by sodium citrate buffer. Following endogenous peroxidase blocking and incubation in normal goat serum (Vector Lab, Burlingame, CA) for 20 mins, these sections were incubated with ERO1A (1:200, LSBio, USA), CEA (Use directly, zsbio, China), CA19-9 (Use directly, zsbio, China) overnight at 4°C and then with the peroxidase-conjugated secondary antibody (abcam, UK). Color development was performed with 3,3′-diaminobenzidine (DAB) and slides were counterstained with hematoxylin. Neutral resin sealing was performed. The results of immunohistochemical staining were observed under a microscope (Olympus Tokyo, Japan).

Yan W., Wang X., Liu T., Chen L., Han L., Xu J., Jin G., Harada K., Lin Z, & Ren X. (2019). Expression of endoplasmic reticulum oxidoreductase 1-α in cholangiocarcinoma tissues and its effects on the proliferation and migration of cholangiocarcinoma cells. Cancer Management and Research, 11, 6727-6739.

+ Open protocol

+ Expand

Evaluation of Halofuginone Hydrobromide Cytotoxicity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Halofuginone hydrobromide (HF) was provided by Shanxi Meixilin Pharmaceutical Co., Ltd. (Yuncheng, China). D-α-tocopherol polyethylene glycol 1000 succinate (TPGS) was purchased from Guangzhou Kafen Biological Technology Co., Ltd. (Guangzhou, China). Fetal bovine serum (FBS) was provided by Gibco Laboratories (Grand, Island). Dulbecco’s modified Eagle’s medium (DMEM), trypsin, penicillin, and streptomycin were obtained from Hyclone Laboratories (Logan, UT). Bcl-2, Bax, and Caspase-3 antibodies were bought from Abcam Biotechnology Co., Ltd. (London, England). TUNEL kit was purchased from Nanjing Kai-ji Biotech Co., Ltd. (Nanjing, China), while the immunohistochemistry kit was bought from Abcam Biotechnology Co., Ltd. (London, England). All other reagents and chemicals were of analytical grade without further purification.

Zuo R., Zhang Y., Chen X., Hu S., Song X., Gao X., Gong J., Ji H., Yang F., Peng L., Fang K., Lv Y., Zhang J., Jiang S, & Guo D. (2022). Orally Administered Halofuginone-Loaded TPGS Polymeric Micelles Against Triple-Negative Breast Cancer: Enhanced Absorption and Efficacy with Reduced Toxicity and Metastasis. International Journal of Nanomedicine, 17, 2475-2491.

+ Open protocol

+ Expand

Biotin-PNA and siRNA Isolation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Biotin-s-s-PNA (CACCACCAC) was purchased from PNA Bio, Inc. (Newbury Park, CA). The PCBP2 siRNA sense strand with sticky end (5’-GUCAGUGUG GCUCUCUUAUGUGGUGGUGdTdT-3’) and the siRNA PCBP2 antisense strand (5’-AUA AGAGAGCCACACUGACdTdT-3’) were purchased from GE Healthcare Dharmacon (Lafayette, CO). Neutravidin and CCl₄ were ordered from Fisher Scientific (Hampton, NH). Bioplex assay kit was purchased from Bio-Rad Laboratories (Hercules, CA). Type I Collagen monoclonal antibody and immunohistochemistry kit were obtained from Abcam (Cambridge, MA).

Jain A., Barve A., Zhao Z., Fetse J.P., Liu H., Li Y, & Cheng K. (2019). Targeted Delivery of an siRNA/PNA Hybrid Nanocomplex Reverses Carbon Tetrachloride‐Induced Liver Fibrosis. Advanced therapeutics, 2(8), 1900046.

+ Open protocol

+ Expand

Salusin-β Immunohistochemistry of Arteries

Check if the same lab product or an alternative is used in the 5 most similar protocols

Salusin-β immunohistochemistry of the arteries was performed with an immunohistochemistry kit (Abcam, MA, United States). Briefly, the arteries were processed into coronal sections (5 μm) and incubated with anti-salusin-β IgG (1:1000, Cloud-Clone Corp, United States) at 4°C overnight. After washing, sections were incubated with biotinylated goat anti-rabbit IgG for 1 h and then stained with DAB according to the manufacturer’s instructions. Sections were covered with mounting medium, and salusin-β immunoreactivity was observed under a light microscope (DP70, Olympus, Tokyo, Japan).

Pan Y., Sun S., Wang X., Chen A., Fei X., Wang W, & Han Y. (2021). Improvement of Vascular Function by Knockdown of Salusin-β in Hypertensive Rats via Nitric Oxide and Reactive Oxygen Species Signaling Pathway. Frontiers in Physiology, 12, 622954.

+ Open protocol

+ Expand

PBMC Immunohistochemistry for Stem Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Noninduced and induced PBMCs were subjected to immunohistochemistry analysis. Specifically, noninduced and induced PBMCs were smeared on a slide, dried, and fixed with 4% paraformaldehyde for 10 minutes. The cells were stained with an Abcam immunohistochemistry kit using primary antibodies against OCT4 and NANOG at 1:50 dilution. The procedures were performed according to the manufacturer’s instructions. DAB was used to develop the staining, and images were captured.

Ruan G.P., Yao X., Lin Q.K., Li Z.A., Cai X.M., Pang R.Q, & Pan X.H. (2020). Transplantation of chicken egg white extract-induced rabbit PBMCs as a treatment for renal ischemia-reperfusion injury in rabbits. PLoS ONE, 15(12), e0244160.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!