Equal amounts of protein samples were subjected to SDS-PAGE on a 12.5% polyacrylamide gel (FUJIFILM Wako Chemicals, Osaka, Japan). After electrophoresis, the proteins were transferred onto PVDF membranes (Merck Millipore, Burlington, MA, USA) and blocked with 5% skim milk in TBST (20 mM Tris, 150 mM NaCl, 50 mM KCl, and 0.05% Tween 20). Anti-TTR (1:500, Cat. No. ab215202, Abcam, Cambridge, UK), anti-LCN2 (1:1000, Cat. No. 26991-1-AP, Proteintech Group Inc., Rosemont, IL, USA), anti-ICAM1 (1:1000, Cat. No. ab179707, Abcam), anti-VCAM1 (1:1000, Cat. No. ab134047, Abcam), and anti-ACTB (1:2000, Cat. No. MA5-15739, Sigma-Aldrich, St. Louis, MO, USA) antibodies in blocking buffer were used as the secondary antibodies. Protein signals were detected using a Western Blot Hyper HRP Substrate (TaKaRa).
Anti actb
Manufactured by Abcam
Sourced in United States
Anti-ACTB is an antibody that targets the ACTB (actin beta) protein. ACTB is a cytoskeletal protein that is ubiquitously expressed in eukaryotic cells and plays a crucial role in various cellular processes such as cell motility, structure, and division. The Anti-ACTB antibody can be used to detect and quantify ACTB in a variety of applications, including Western blotting, immunohistochemistry, and flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Western blot hyper hrp substrate, by Takara Bio (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Aqp 005, by Abcam (1 mentions)
Fluorchem 8900 imaging system, by Bio-Techne (1 mentions)
Anti lamin a c sc 20681, by Santa Cruz Biotechnology (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Anti hdac2, by Cell Signaling Technology (1 mentions)
Ecl western blotting protocol, by GE Healthcare (1 mentions)
Anti dicer, by Merck Group (1 mentions)
Anti ago2, by Merck Group (1 mentions)
Las 4000mini image reader, by GE Healthcare (1 mentions)
Anti phospho histone h3, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Anti irf9, by Santa Cruz Biotechnology (1 mentions)
Anti isg15, by Cell Signaling Technology (1 mentions)
Ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
Pvdf membrane, by Bio-Rad (1 mentions)
Anti gpx4, by Abcam (1 mentions)
5 protocols using anti actb
1
Quantitative Protein Profiling via Western Blot
2
Protein Analysis via Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blots were performed as previously described [8 (link)]. Primary antibodies (all rabbit) were anti-AQP5 (Alomone Labs AQP-005), anti-CAV1 (Abcam ab2910), anti-pro-SFTPC (Millipore AB3786), anti-ACTB (Abcam AB8226), anti-PDPN (Developmental Studies Hybridoma Bank #8.1.1), and anti-LAMIN A/C (sc-20,681, Santa Cruz Biotechnology). Blots were analyzed by chemiluminescence and visualized by West Fempto Super Sensitivity Kit (Thermo Scientific) with a FluorChem 8900 Imaging System (Alpha Innotech).
3
Western Blotting Technique for Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For western blots, samples were separated on SDS-polyacrylamide gel electrophoresis gels and then transferred to Polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA). Membranes were processed following the ECL western blotting protocol (GE Healthcare). These antibodies were used in western blots: anti-CENPC1 (Abcam, 1:1 000 dilution, cat no. ab50974), anti-Phospho-Histone H3 (Cell Signaling Technology, 1:1 000 dilution, cat no. 9706), anti-ACTB (Abcam, 1:1 000 dilution, cat no. ab8227), anti-Dicer (Sigma, 1:2 000 dilution, cat no. SAB4200087), anti-GAPDH (Cell Signaling Technology, Danvers, MA, USA, 1:2 000 dilution, cat no. 3683), anti-AGO2 (Sigma, 1:2 000 dilution, cat no. SAB4200085) and anti-HDAC2 (Cell Signaling Technology, 1:1 000 dilution, cat no. 5113). Images were taken with LAS-4000mini Image Reader (GE Healthcare).
4
Protein Expression Analysis by Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein samples were denatured, separated by SDS/PAGE and transferred onto PVDF membrane (Biorad). Membranes were blocked followed by incubation with primary antibodies anti-ZNF677 (1:100, Abcam), anti-IRF9 (1:50, Santa Cruz Biotechnology), anti-ISG15 (1:1000, Cell Signaling), anti-CSH1 (1:375, Thermo Scientific), anti-ACTB (1:200, Abcam) and anti-GAPDH (0.05mg/ml, Sigma Aldrich). Appropriate secondary HRP antibodies were used and membranes were visualized using ECL Western blotting substrate (Thermo Scientific).
5
Quantifying Intestinal Protein Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentration of protein extracted from mouse intestinal tissue samples was determined by Western blotting. Briefly, the gut tissues were lysed by prechilled RIPA lysis buffer containing with protease and phosphatase inhibitor cocktail and PMSF for 10 minutes. The protein was quantified using BCA method, and equal amount of them were separated on 15% SDS PAGE followed by transferred to NC membranes. The membranes were blocked in 5% non-fat milk for 1.5 hours and then incubated with primary antibodies overnight: rabbit anti-Ferritin Heavy Chain (Cat. ab183781, Abcam, dilution 1:1000), anti-Gpx4 (Cat. ab125066, Abcam, dilution 1:1000), anti-ACTB (Cat. AP0060, Bioworld, 1:5000). Then the membrane was washed by TBST (0.1% Tween 20) and incubated with secondary antibody (Cat.RM3002, Beijing Ray Antibody Biotech, 1:10000). The ECL detection reagent was used to visualize the protein bands in the membrane. The quantity of the bands was analyzed with ImageJ.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!