The largest database of trusted experimental protocols

3 protocols using rabbit anti myc tag 71d10

1

Single-cell Immunophenotyping and Glucose Uptake

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions were prepared and stained as previously described (Lin et al., 2016 (link)). Antibodies used include rabbit anti-myc tag (71D10, Cell Signaling Technology), mouse anti-myc tag (4A6, Millipore), TCF1 (C63D9, Cell Signaling Technology), Pax5 (1H9, eBioscience), IgM (Il/41, eBioscience), IgD (11-26c.2a, BioLegend), CD23 (B3B4, eBioscience), CD4 (RM405, BioLegend), CD8α (53-6.7, eBioscience), and Gr1 (RB6-8C5, BD PharMingen). Glucose uptake was determined by staining with the fluorescent glucose analog 2-NBDG (Cayman, 100 μM) in glucose-free RPMI.
+ Open protocol
+ Expand
2

Antibodies and Small Molecules for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rabbit anti-TULP1 (ab97281) and rabbit anti-TULP3 antibody (ab155317) were obtained from Abcam. HDAC Inhibitor XXIV (OSU-HDAC-44) was obtained from Calbiochem. Cycloheximide (2112S), MG-132 (2194S), rabbit antiacetylated lysine (#9441S), rabbit anti-beta actin (13E5, #4970S), rabbit anti-CUL3 (#2759S), rabbit anti-HA tag (C29F4, #3724T), mouse anti-HDAC1 (10E2, #5356S), antimouse IgG, HRP linked antibody (7076S), rabbit anti-Myc tag (71D10, #2278S), rabbit anti-P300 (D1M7C, #70088S), rabbit anti-Rad18 (D2B8, #9040S), rabbit anti-SIRT1 (C14H4, #2496S), anti-rabbit IgG, HRP linked antibody (7074S), rabbit antiubiquitin (#3933S), Trichostatin A (9950S) were obtained from Cell Signaling Technology, Inc. Rabbit anti-PP6R3 (16944-1-AP) was obtained from Proteintech. Anacardic Acid (A7236), Anti-FLAG (M2, #F1804), C646 (SML0002), EX-527 (E7034), MC1568 (M1824), mouse antiacetyl lysine (4G12, 05-515), mouse anti-TULP2 (2B5, WH0007288M3), MS-275 (EPS002), and nicotinamide (N3376) were obtained from Sigma Aldrich.
+ Open protocol
+ Expand
3

Single-cell Immunophenotyping and Glucose Uptake

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions were prepared and stained as previously described (Lin et al., 2016 (link)). Antibodies used include rabbit anti-myc tag (71D10, Cell Signaling Technology), mouse anti-myc tag (4A6, Millipore), TCF1 (C63D9, Cell Signaling Technology), Pax5 (1H9, eBioscience), IgM (Il/41, eBioscience), IgD (11-26c.2a, BioLegend), CD23 (B3B4, eBioscience), CD4 (RM405, BioLegend), CD8α (53-6.7, eBioscience), and Gr1 (RB6-8C5, BD PharMingen). Glucose uptake was determined by staining with the fluorescent glucose analog 2-NBDG (Cayman, 100 μM) in glucose-free RPMI.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!