Antibodies directed against the following human surface proteins were used: HLA-DR (L243), CD11c (B-ly6), DC-SIGN (DCN46),3CXCR1 (5A12), β7 (FIB504), α4 (9F10), cytokeratin (CAM5.2), CD45 (2D1, all from Becton Dickinson, San Jose, CA); BDCA-1/CD1c (L161), SIRP-α (SE5A5, both from Biolegend, San Diego, CA); CCL25 (rabbit polyclonal, from AbD Serotec, Raleigh, NC); and CD103 (B-Ly7, eBioscience, San Diego, CA). Our lineage cocktail contained antibodies to CD3, CD14, CD19, and CD20 (all from Becton Dickinson). A LIVE/DEAD® yellow dye (Life Technologies, Carlsbad, CA) or propidiumiodide were used to exclude dead cell populations. The capacity of live cells to convert retinaldehyde to RA was measured using the flow cytometry-based Aldefluor™ assay according to the manufacturer’s protocol (Stemcell Technologies, Vancouver, BC, Canada). The Aldefluor™ assay employs a fluorescent non-toxic aminoacetaldehyde, which freely diffuses into intact and viable cells and is converted by ALDH into an aminoacetate that is retained inside the cells. An LSRII flow cytometer (Becton Dickinson) and FlowJo 7.6.5 software (TreeStar, Ashland, OR) were used for analysis. Immunofluorescence staining of frozen gastric and intestinal sections was performed as described previously (26 (link)).
Cd103 b ly7
Manufactured by Thermo Fisher Scientific
The CD103 (B-Ly7) is a laboratory instrument for the detection and analysis of specific cell surface markers. It is designed to provide reliable and accurate measurements of the expression levels of the CD103 antigen on various cell types. The core function of the CD103 (B-Ly7) is to facilitate the characterization and identification of cells expressing the CD103 marker, which is commonly found on certain lymphocyte subsets.
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Lab products found in correlation
2 protocols using cd103 b ly7
1
Multifaceted Analysis of Immune Cells
2
Multifaceted Analysis of Immune Cells
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