Animals were given a ketamine/xylazine overdose (2×100 mg/kg ketamine+ xylazine 10 mg/kg, intraperitoneal) and were transcardially perfused with phosphate buffered saline. Cerebella were dissected and stored at −80°C before use. Protein extracts and western blots were performed as previously described45 (link). For these experiments, 15 μg of total protein extracts were run in the gel. A monoclonal anti-Tau (Tau46; 1:1,000; Cell Signaling Technology) or mouse monoclonal anti-β-actin antibody (clone AC74; 1:5,000; Sigma-Aldrich), diluted in blocking solution (5% non-fat milk in TBS-T), were used. An alkaline phosphatase-linked antibody specific to mouse IgG (1:20,000, Amersam Biosciences, GE Healthcare, UK) was used as secondary antibody. GraphPad Prism v.8.0.1 was used to analyze animal data.
Mouse monoclonal anti β actin antibody clone ac74
Manufactured by Merck Group
Sourced in United Kingdom
The Mouse monoclonal anti-β-actin antibody (clone AC74) is a laboratory reagent used for the detection and quantification of β-actin, a ubiquitous cytoskeletal protein, in various sample types. This antibody is suitable for applications such as Western blotting, immunohistochemistry, and immunocytochemistry.
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Lab products found in correlation
3 protocols using mouse monoclonal anti β actin antibody clone ac74
1
Cerebellum Protein Extraction and Western Blot Analysis
2
Cerebral Protein Extraction and Western Blotting
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Animals were given a ketamine/xylazine overdose (2 × 100 mg/kg ketamine + xylazine 10 mg/kg, intraperitoneal) and were transcardially perfused with phosphate buffered saline. Cerebella were dissected and stored at −80°C before use. Protein extracts and western blots were performed as previously described [45 (link)]. For these experiments, 15 µg of total protein extracts were run in the gel. A monoclonal anti‐tau (Tau46; 1:1000; Cell Signaling Technology) or mouse monoclonal anti‐β‐actin antibody (clone AC74; 1:5000; Sigma‐Aldrich), diluted in blocking solution (5% non‐fat milk in TBS‐T), was used. An alkaline phosphatase‐linked antibody specific to mouse immunoglobulin G (1:20,000, Amersham Biosciences, GE Healthcare, UK) was used as secondary antibody. GraphPad Prism v.8.0.1 was used to analyse animal data.
3
Immunodetection of Mitochondrial Proteins
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The anti-Mieap antibody was prepared as described previously.1 (link) The other primary antibodies used in this study were mouse monoclonal anti-TOM20 antibody (sc-17764, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit polyclonal anti-LAMP1 antibody (sc-5570, Santa Cruz), mouse monoclonal anti-BNIP3 antibody (ANa40, Abcam, Cambridge, UK) and mouse monoclonal anti-β-actin antibody (clone AC-74, Sigma-Aldrich, St Louis, MO, USA).
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