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Biacore evaluation software 3

Manufactured by GE Healthcare
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Biacore Evaluation software 3.0 is a data analysis tool designed for use with Biacore biosensor systems. The software provides functionality for processing and analyzing data generated from Biacore experiments, enabling users to interpret their results.

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Lab products found in correlation

Prism 8, by GraphPad (2 mentions) Amine coupling kit, by GE Healthcare (1 mentions) Biacore t200, by GE Healthcare (1 mentions)

Spelling variants of this product

Biacore Evaluation Software (80 citations) Biacore T200 Evaluation Software 3.1 (15 citations) Evaluation Software (2 citations) BIAcore T200 evaluation software version 3.1 (2 citations) Biacore Insight Evaluation software v.3.0 (2 citations)

3 protocols using biacore evaluation software 3

1

SPR Analysis of Protein Interactions

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Surface Plasmon Resonance (SPR) experiments were performed using a Biacore T200 (GE Healthcare) instrument by MEDICILON (Shanghai) co., Ltd. The proteins were immobilized on a CM5 (GE Healthcare) chip through amine coupling using the amine coupling kit (GE Healthcare) resulting in immobilization 13742.8 RU level. The samples flowed over the surface with 30 µL min–1 for 180 s binding time and 180 s dissociation time. The obtained data were analyzed by the Biacore Evaluation Software 3.0 (GE Healthcare).
Xu Y., Yu Q., Wang P., Wu Z., Zhang L., Wu S., Li M., Wu B., Li H., Zhuang H., Zhang X., Huang Y., Gan X, & Xu R. (2022). A Selective Small‐Molecule c‐Myc Degrader Potently Regresses Lethal c‐Myc Overexpressing Tumors. Advanced Science, 9(8), 2104344.
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2

Determining mAb Binding Kinetics

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The independent experiment replicates were indicated in the figure legends. Half-maximal inhibitory concentrations (IC50) of mAbs were calculated using GraphPad Prism 8.0 software by log (inhibitor) vs. normalized response - Variable slope (four parameters) model. The values of binding affinity (KD) of mAbs were calculated using Biacore Evaluation software 3.0 (GE Healthcare) by Multi-cycle kinetics/affinity model. We did not perform any statistical analysis in this study.
Li Y., Fan Q., Zhou B., Shen Y., Zhang Y., Cheng L., Qi F., Song S., Guo Y., Yan R., Ju B, & Zhang Z. (2022). Structural and functional analysis of an inter-Spike bivalent neutralizing antibody against SARS-CoV-2 variants. iScience, 25(6), 104431.
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3

Antibody Characterization Protocol

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The independent experiment replicates were indicated in the figure legends. Half-maximal inhibitory concentrations (IC50) of mAbs were calculated using GraphPad Prism 8.0 software by log (inhibitor) vs. normalized response - Variable slope (four parameters) model. Curves for ADCC activities were fitted after log transformation of antibody concentration using GraphPad Prism 8.0 software by non-linear regression analysis. The values of binding affinity (KD) of mAbs were calculated using Biacore Evaluation software 3.0 (GE Healthcare) by Multi-cycle kinetics/affinity model. We did not perform any statistical analysis in this study.
Wang M., Fan Q., Zhou B., Ye H., Shen S., Yu J., Cheng L., Ge X., Ju B, & Zhang Z. (2022). A key F27I substitution within HCDR1 facilitates the rapid maturation of P2C-1F11-like neutralizing antibodies in a SARS-CoV-2-infected donor. Cell Reports, 40(11), 111335.
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