Quantitative rt pcr primers and probes

Recombinant human BMP2 was purchased from R&D Systems (Minneapolis, MN). E2 was purchased from Steraloids, Inc. (Newport, RI). Estradiol 17β cypionate was purchased from Pfizer Company (Kalamazoo, MI). Phenol red-free DMEM, linolenic acid, bovine serum albumin (BSA), and fine chemicals were purchased from Sigma Chemical Company (St. Louis, MO). LDN193,189 was purchased from Sigma Chemical Co. (St. Louis, MO), ICI182,780 was purchased from Tocris Bioscience (Bristol, UK), and human holo-transferrin was obtained from BD pharmaceuticals (San Jose, CA). Plastic embedding medium and supplies were obtained from Electron Microscopy Sciences (Hatfield, Pa). Quantitative RT-PCR primers and probes were synthesized by Sigma Chemical Company. RNeasy mini kit and Taq DNA polymerase were from Qiagen, Inc. (Valencia, CA). All other molecular biology grade chemicals were obtained from Sigma Chemical Company or Fisher Scientific (Pittsburgh, PA).

Antibodies to BMP2, SYCP3, MVH, γHis2A.X and MCM2 were obtained from Abcam (Cambridge, MA). Alexa-conjugated secondary antibodies were obtained from Invitrogen (Carlsbad, CA). Plastic embedding medium was obtained from Electron Microscopy Sciences (Hatfield, Pa). Tissue-Tek Optimal Cutting Temperature (OCT) compound was obtained from Sacura Finetek USA Inc. (Torrance, CA). Phenol red-free DMEM, linolenic acid, BSA, and fine chemicals were purchased from Sigma Chemical Company (St. Louis, MO). LDN 193,189 was purchased from Sigma Chemical Co. (St. Louis, MO). DMH1 was purchased from Tocris Bioscience (Bristol, UK). Human holo-transferrin was obtained from BD pharmaceuticals (San Jose, CA). Quantitative RT-PCR primers and probes were synthesized by Sigma Chemical Company. RNeasy mini kit and Taq DNA polymerase were from Qiagen, Inc. (Valencia, CA). The terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) kit was from Roche pharmaceuticals (Mannheim, Germany). All other molecular biology grade chemicals were obtained from Sigma Chemical Company or Fisher Scientific (Pittsburgh, PA).