Recombinant ige

Compound 48/80, phorbol 12-myristate 13-acetate (PMA), calcium Ionophore (CI) A23187, avidin peroxidase, and DNCB were purchased from Sigma-Aldrich Chemical Corp., (St. Louis, MO, USA). IMDM was from GE Healthcare Life Science (Little Chalfont, UK) and fetal bovine serum (FBS) was obtained from JR Scientific, Inc., (Woodland, CA, USA). Anti-human IL-4/IL-10, recombinant IL-4/IL-10, biotinylated IL-4/IL-10, anti-mouse IgE, recombinant IgE, and biotinylated IgE were purchased from Pharmingen (San Diego, CA, USA). Human TNF-α, IL-1β, IL-6, IL-8, mouse TNF-α, and IL-6 ELISA kits were purchased from BD Biosciences (San Diego, CA, USA) and the histamine assay kit was obtained from Abnova Corp., (Taipei, Taiwan). Antibodies (Abs) were purchased from Santa Cruz Biotechnology, Inc., (Santa Cruz, CA, USA).

2, 4-dinitrofluorobenzene (DNFB), compound 48/80, 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), PMA, and A23187 were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Avidin peroxidase (AP), fetal bovine serum (FBS), Roswell Park Memorial Institute (RPMI) 1640, and Iscove's Modified Dulbecco's Medium (IMDM) were purchased from Gibco BRL (Grand Island, NY, USA). Anti-human TNF-α/IL-6/IL-8, recombinant TNF-α/IL-6/IL-8, biotinylated TNF-α/IL-6/IL-8, anti-mouse IgE/IL-1β, recombinant IgE, IL-1β, biotinylated IgE, and IL-1β were purchased from BD Pharmingen (San Diego, CA, USA). Anti-phospho p38 antibody was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Anti-phospho ERK, JNK, IκBα, anti-p38, JNK, NF-κB, histone, and GAPDH antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Anti-ERK, and α-tubulin antibodies were purchased from Bioworld Technology (Minneapolis, MN, USA).